Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials
A pilot-scale production of lipase using palm oil mill effluent (POME) as a fermentation basal medium was carried out, and parameters for immobilization of the produced lipase were optimized. Lipase production in a 300-L bioreactor was performed using two proposed strategies, constant power per volu...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Japan Oil Chemists' Society
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/38191/ http://irep.iium.edu.my/38191/ http://irep.iium.edu.my/38191/ http://irep.iium.edu.my/38191/1/Paper-JOS-devi.pdf http://irep.iium.edu.my/38191/4/38191-Pilot-scale%20production%20of%20lipase%20using%20palm%20oil%20mill%20effluent%20as%20a%20basal%20medium_SCOPUS.pdf |
| Summary: | A pilot-scale production of lipase using palm oil mill effluent (POME) as a fermentation basal medium was carried out, and parameters for immobilization of the produced lipase were optimized. Lipase production in a 300-L bioreactor was performed using two proposed strategies, constant power per volume (P/V) and constant tip speed. Moreover, lipase immobilization on different materials was also investigated. Lipase production was performed using liquid-state bioconversion of POME as the medium and Candida cylindracea as the inoculum. The fermentation medium was composed of 1% total suspended solids (TSS) of POME, 0.5% (w/v) peptone, 0.7% (v/v) Tween-80, and 2.2% inoculum. The medium composition was decided on the basis of the medium optimization results of a previous study. The fermentation was carried out for 48 h at 30°C and pH 6. The maximum lipase production was 5.72U/mL and 21.34 U/mL, obtained from the scale-up strategies of constant tip speed and P/V, respectively. Four accessible support materials were screened for their potential use in immobilization. The most suitable support material was found to be activated carbon, with a maximum immobilization of 94%. |
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