Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms

Kraits (Bungarus spp.) are highly venomous elapids that are only found in Asia. In the current study, 103 and 86 different proteins were identified from Bungarus candidus and Bungarus fasciatus venoms, respectively. These proteins were classified into 18 different venom protein families. Both venoms...

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Main Authors:	Ahmad Rusmili, Muhamad Rusdi, Ting, Tee Yee, Mustafa, Mohd Rais, Hodgson, Wayne C., Othman, Iekhsan
Format:	Article
Language:	English English English
Published:	Elsevier Ltd. 2014
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/38142/ http://irep.iium.edu.my/38142/ http://irep.iium.edu.my/38142/ http://irep.iium.edu.my/38142/1/38142_Proteomic%20characterization%20and%20comparison.pdf http://irep.iium.edu.my/38142/2/38142_Proteomic%20characterization%20and%20comparison_SCOPUS.pdf http://irep.iium.edu.my/38142/3/38142_Proteomic%20characterization%20and%20comparison_WOS.pdf

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spelling	iium-381422017-09-20T08:08:10Z http://irep.iium.edu.my/38142/ Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms Ahmad Rusmili, Muhamad Rusdi Ting, Tee Yee Mustafa, Mohd Rais Hodgson, Wayne C. Othman, Iekhsan QD Chemistry Kraits (Bungarus spp.) are highly venomous elapids that are only found in Asia. In the current study, 103 and 86 different proteins were identified from Bungarus candidus and Bungarus fasciatus venoms, respectively. These proteins were classified into 18 different venom protein families. Both venoms were found to contain a high percentage of three finger toxins, phospholipase A2 enzymes and Kunitz-type inhibitors. Smaller number of high molecular weight enzymes such as L-amino acid oxidase, hyaluronidases, and acetylcholinesterase were also detected in the venoms. We also detected some unique proteins that were not known to be present in these venoms. The presence of a natriuretic peptide, vespryn, and serine protease families was detected in B. candidus venom. We also detected the presence of subunit A and B of β-bungarotoxin and α-bungarotoxin which had not been previously found in B. fasciatus venom. Understanding the proteome composition of Malaysian krait species will provide useful information on unique toxins and proteins which are present in the venoms. This knowledge will assist in the management of krait envenoming. In addition, these proteins may have potential use as research tools or as drug-design templates. Elsevier Ltd. 2014-10-14 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38142/1/38142_Proteomic%20characterization%20and%20comparison.pdf application/pdf en http://irep.iium.edu.my/38142/2/38142_Proteomic%20characterization%20and%20comparison_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/38142/3/38142_Proteomic%20characterization%20and%20comparison_WOS.pdf Ahmad Rusmili, Muhamad Rusdi and Ting, Tee Yee and Mustafa, Mohd Rais and Hodgson, Wayne C. and Othman, Iekhsan (2014) Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms. Journal of Proteomics, 110. pp. 129-144. ISSN 1874-3919 http://www.sciencedirect.com/science/article/pii/S1874391914003996 10.1016/j.jprot.2014.08.001
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QD Chemistry
spellingShingle	QD Chemistry Ahmad Rusmili, Muhamad Rusdi Ting, Tee Yee Mustafa, Mohd Rais Hodgson, Wayne C. Othman, Iekhsan Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms
description	Kraits (Bungarus spp.) are highly venomous elapids that are only found in Asia. In the current study, 103 and 86 different proteins were identified from Bungarus candidus and Bungarus fasciatus venoms, respectively. These proteins were classified into 18 different venom protein families. Both venoms were found to contain a high percentage of three finger toxins, phospholipase A2 enzymes and Kunitz-type inhibitors. Smaller number of high molecular weight enzymes such as L-amino acid oxidase, hyaluronidases, and acetylcholinesterase were also detected in the venoms. We also detected some unique proteins that were not known to be present in these venoms. The presence of a natriuretic peptide, vespryn, and serine protease families was detected in B. candidus venom. We also detected the presence of subunit A and B of β-bungarotoxin and α-bungarotoxin which had not been previously found in B. fasciatus venom. Understanding the proteome composition of Malaysian krait species will provide useful information on unique toxins and proteins which are present in the venoms. This knowledge will assist in the management of krait envenoming. In addition, these proteins may have potential use as research tools or as drug-design templates.
format	Article
author	Ahmad Rusmili, Muhamad Rusdi Ting, Tee Yee Mustafa, Mohd Rais Hodgson, Wayne C. Othman, Iekhsan
author_facet	Ahmad Rusmili, Muhamad Rusdi Ting, Tee Yee Mustafa, Mohd Rais Hodgson, Wayne C. Othman, Iekhsan
author_sort	Ahmad Rusmili, Muhamad Rusdi
title	Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms
title_short	Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms
title_full	Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms
title_fullStr	Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms
title_full_unstemmed	Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms
title_sort	proteomic characterization and comparison of malaysian bungarus candidus and bungarus fasciatus venoms
publisher	Elsevier Ltd.
publishDate	2014
url	http://irep.iium.edu.my/38142/ http://irep.iium.edu.my/38142/ http://irep.iium.edu.my/38142/ http://irep.iium.edu.my/38142/1/38142_Proteomic%20characterization%20and%20comparison.pdf http://irep.iium.edu.my/38142/2/38142_Proteomic%20characterization%20and%20comparison_SCOPUS.pdf http://irep.iium.edu.my/38142/3/38142_Proteomic%20characterization%20and%20comparison_WOS.pdf
first_indexed	2023-09-18T20:54:45Z
last_indexed	2023-09-18T20:54:45Z
_version_	1777410229477048320

Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms

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