Effects of trigonella foenum-graecum L.(Fenugreek) seeds aqueous extract on reproductive system of female rats

The purpose of this study was to evaluate the effects of fenugreek seeds aqueous (FSA) extract on fertility of female rats by assessing estrous cycles, determining serum reproductive hormone concentrations and observing their ovarian tissue histology as well as determining the reversibility of these...

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Main Authors:	Mohamed Karim, Norbaiyah, Allow, Ahmed Kaid Naji, Al-Ani, Imad Matloub Dally, Mohd Hassan, Norazian
Format:	Conference or Workshop Item
Language:	English English English
Published:	2014
Subjects:	QM Human anatomy
Online Access:	http://irep.iium.edu.my/38060/ http://irep.iium.edu.my/38060/ http://irep.iium.edu.my/38060/2/Fenugreek_Poster_PPSIC_2014.pdf http://irep.iium.edu.my/38060/5/Prograam_book.jpg http://irep.iium.edu.my/38060/6/Fenugreek.jpg

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recordtype	eprints
spelling	iium-380602018-06-11T05:32:08Z http://irep.iium.edu.my/38060/ Effects of trigonella foenum-graecum L.(Fenugreek) seeds aqueous extract on reproductive system of female rats Mohamed Karim, Norbaiyah Allow, Ahmed Kaid Naji Al-Ani, Imad Matloub Dally Mohd Hassan, Norazian QM Human anatomy The purpose of this study was to evaluate the effects of fenugreek seeds aqueous (FSA) extract on fertility of female rats by assessing estrous cycles, determining serum reproductive hormone concentrations and observing their ovarian tissue histology as well as determining the reversibility of these effects once the treatment was withdrawn. Twenty four 8-week-old Sprague Dawley female rats with normal estrous cycle were divided into three groups (A, B and C ). Group A (control group) were treated with distilled water as vehicle. Groups B and C (treatment group) were administered orally with 500 mg/kg body weight of FSA extract for 15 days. Daily vaginal smear cytology was examined. Blood samples were taken on day 16 for evaluation of the reproductive hormones assay. Ovaries of the animals were removed for histological study. Group C (reversible group) were observed further for 15 days for reversibility. The duration of days of proestrus in treatment group were significantly prolonged (P<0.001)and the diestrus phase was significantly shortened (P<0.001). Administration of 500 mg/kg FSA extract led to a decrease in the serum concentration of estrogen (P<0.001), progesterone (P=0.021), FSH (P=0.416) and LH (P=0.381) while serum prolactin concentration was significantly higher (P<0.001). More degenerative follicles and congested blood vessel were observed in the treated group versus normal controls. After 15 days of treatment withdrawal, serum estrogen, progesterone, FSH and LH concentration were not significantly different (P>0.192) in compare to the control group. The estrous cycles slowly returned to normal cycle after 10-15 days of withdrawal and no histopathological findings were noted in the ovarian tissues. FSA extract showed reversible anti fertility effects by causing abnormalities in the estrous cycles regulation, alterations in concentration of reproductive hormones and histopathological changes in the ovarian tissues. 2014-08 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/38060/2/Fenugreek_Poster_PPSIC_2014.pdf application/pdf en http://irep.iium.edu.my/38060/5/Prograam_book.jpg application/pdf en http://irep.iium.edu.my/38060/6/Fenugreek.jpg Mohamed Karim, Norbaiyah and Allow, Ahmed Kaid Naji and Al-Ani, Imad Matloub Dally and Mohd Hassan, Norazian (2014) Effects of trigonella foenum-graecum L.(Fenugreek) seeds aqueous extract on reproductive system of female rats. In: Pharmacology & Physiology International Scientific Congress (2014), 22-24 August 2014, Kuala Lumpur. (Unpublished) http://www.msppscientificmeetings.com/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QM Human anatomy
spellingShingle	QM Human anatomy Mohamed Karim, Norbaiyah Allow, Ahmed Kaid Naji Al-Ani, Imad Matloub Dally Mohd Hassan, Norazian Effects of trigonella foenum-graecum L.(Fenugreek) seeds aqueous extract on reproductive system of female rats
description	The purpose of this study was to evaluate the effects of fenugreek seeds aqueous (FSA) extract on fertility of female rats by assessing estrous cycles, determining serum reproductive hormone concentrations and observing their ovarian tissue histology as well as determining the reversibility of these effects once the treatment was withdrawn. Twenty four 8-week-old Sprague Dawley female rats with normal estrous cycle were divided into three groups (A, B and C ). Group A (control group) were treated with distilled water as vehicle. Groups B and C (treatment group) were administered orally with 500 mg/kg body weight of FSA extract for 15 days. Daily vaginal smear cytology was examined. Blood samples were taken on day 16 for evaluation of the reproductive hormones assay. Ovaries of the animals were removed for histological study. Group C (reversible group) were observed further for 15 days for reversibility. The duration of days of proestrus in treatment group were significantly prolonged (P<0.001)and the diestrus phase was significantly shortened (P<0.001). Administration of 500 mg/kg FSA extract led to a decrease in the serum concentration of estrogen (P<0.001), progesterone (P=0.021), FSH (P=0.416) and LH (P=0.381) while serum prolactin concentration was significantly higher (P<0.001). More degenerative follicles and congested blood vessel were observed in the treated group versus normal controls. After 15 days of treatment withdrawal, serum estrogen, progesterone, FSH and LH concentration were not significantly different (P>0.192) in compare to the control group. The estrous cycles slowly returned to normal cycle after 10-15 days of withdrawal and no histopathological findings were noted in the ovarian tissues. FSA extract showed reversible anti fertility effects by causing abnormalities in the estrous cycles regulation, alterations in concentration of reproductive hormones and histopathological changes in the ovarian tissues.
format	Conference or Workshop Item
author	Mohamed Karim, Norbaiyah Allow, Ahmed Kaid Naji Al-Ani, Imad Matloub Dally Mohd Hassan, Norazian
author_facet	Mohamed Karim, Norbaiyah Allow, Ahmed Kaid Naji Al-Ani, Imad Matloub Dally Mohd Hassan, Norazian
author_sort	Mohamed Karim, Norbaiyah
title	Effects of trigonella foenum-graecum L.(Fenugreek) seeds aqueous extract on reproductive system of female rats
title_short	Effects of trigonella foenum-graecum L.(Fenugreek) seeds aqueous extract on reproductive system of female rats
title_full	Effects of trigonella foenum-graecum L.(Fenugreek) seeds aqueous extract on reproductive system of female rats
title_fullStr	Effects of trigonella foenum-graecum L.(Fenugreek) seeds aqueous extract on reproductive system of female rats
title_full_unstemmed	Effects of trigonella foenum-graecum L.(Fenugreek) seeds aqueous extract on reproductive system of female rats
title_sort	effects of trigonella foenum-graecum l.(fenugreek) seeds aqueous extract on reproductive system of female rats
publishDate	2014
url	http://irep.iium.edu.my/38060/ http://irep.iium.edu.my/38060/ http://irep.iium.edu.my/38060/2/Fenugreek_Poster_PPSIC_2014.pdf http://irep.iium.edu.my/38060/5/Prograam_book.jpg http://irep.iium.edu.my/38060/6/Fenugreek.jpg
first_indexed	2023-09-18T20:54:37Z
last_indexed	2023-09-18T20:54:37Z
_version_	1777410221084246016

Effects of trigonella foenum-graecum L.(Fenugreek) seeds aqueous extract on reproductive system of female rats

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