Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo

The decreased cancer risk associated with consumption of olive oilmay be due to the presence of phenolics which can modulate pathways including apoptosis and invasion that are relevant to carcinogenesis. We have previously shown that a virgin olive oil phenolics extract (OVP) inhibited invasion of...

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Main Authors:	Hashim, Yumi Zuhanis Has-Yun, Worthington, Jenny, Allsopp, Philip, Ternan, Nigel G., Brown, Emma M., McCann, Mark J., Rowland, Ian R., Esposto, Sonia, Servili, Maurizio, Gill, Chris I. R.
Format:	Article
Language:	English English
Published:	The Royal Society of Chemist 2014
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/38022/ http://irep.iium.edu.my/38022/ http://irep.iium.edu.my/38022/ http://irep.iium.edu.my/38022/1/Paper_-_Hashim_et_al_2014_%28Food%26Func%29.pdf http://irep.iium.edu.my/38022/4/38022_Virgin%20olive%20oil_scopus.pdf

id	iium-38022
recordtype	eprints
spelling	iium-380222017-09-19T02:52:10Z http://irep.iium.edu.my/38022/ Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo Hashim, Yumi Zuhanis Has-Yun Worthington, Jenny Allsopp, Philip Ternan, Nigel G. Brown, Emma M. McCann, Mark J. Rowland, Ian R. Esposto, Sonia Servili, Maurizio Gill, Chris I. R. Q Science (General) The decreased cancer risk associated with consumption of olive oilmay be due to the presence of phenolics which can modulate pathways including apoptosis and invasion that are relevant to carcinogenesis. We have previously shown that a virgin olive oil phenolics extract (OVP) inhibited invasion of HT115 colon cancer cells in vitro. In the current study we assessed the in vitro effects of OVP (25 mg mL-1) on HT115 cell migration, spreading and integrin expression. Furthermore, the anti-metastatic activity of OVP – at a dose equivalent to 25 mg per kg per day for 2, 8 or 10 weeks – was assessed in a Severe Combined ImmunoDeficiency (SCID) Balb-c mouse model. After 24 h OVP did not inhibit cell migration but significantly reduced cell spreading on fibronectin (65% of control; p < 0.05) and expression of a range of a and b integrins was modulated. In vivo, OVP by gavage significantly (p < 0.05) decreased not only tumour volume but also the number of metastases in SCID Balb-c mice. Collectively, the data suggest that – possibly through modulation of integrin expression – OVP decreases invasion in vitro and also inhibits metastasis in vivo. The Royal Society of Chemist 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38022/1/Paper_-_Hashim_et_al_2014_%28Food%26Func%29.pdf application/pdf en http://irep.iium.edu.my/38022/4/38022_Virgin%20olive%20oil_scopus.pdf Hashim, Yumi Zuhanis Has-Yun and Worthington, Jenny and Allsopp, Philip and Ternan, Nigel G. and Brown, Emma M. and McCann, Mark J. and Rowland, Ian R. and Esposto, Sonia and Servili, Maurizio and Gill, Chris I. R. (2014) Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo. Food and Function, 5 (7). pp. 1513-1519. ISSN 2042-6496 http://pubs.rsc.org/en/Content/ArticleLanding/2014/FO/c4fo00090k#!divAbstract 10.1039/c4fo00090k
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Hashim, Yumi Zuhanis Has-Yun Worthington, Jenny Allsopp, Philip Ternan, Nigel G. Brown, Emma M. McCann, Mark J. Rowland, Ian R. Esposto, Sonia Servili, Maurizio Gill, Chris I. R. Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo
description	The decreased cancer risk associated with consumption of olive oilmay be due to the presence of phenolics which can modulate pathways including apoptosis and invasion that are relevant to carcinogenesis. We have previously shown that a virgin olive oil phenolics extract (OVP) inhibited invasion of HT115 colon cancer cells in vitro. In the current study we assessed the in vitro effects of OVP (25 mg mL-1) on HT115 cell migration, spreading and integrin expression. Furthermore, the anti-metastatic activity of OVP – at a dose equivalent to 25 mg per kg per day for 2, 8 or 10 weeks – was assessed in a Severe Combined ImmunoDeficiency (SCID) Balb-c mouse model. After 24 h OVP did not inhibit cell migration but significantly reduced cell spreading on fibronectin (65% of control; p < 0.05) and expression of a range of a and b integrins was modulated. In vivo, OVP by gavage significantly (p < 0.05) decreased not only tumour volume but also the number of metastases in SCID Balb-c mice. Collectively, the data suggest that – possibly through modulation of integrin expression – OVP decreases invasion in vitro and also inhibits metastasis in vivo.
format	Article
author	Hashim, Yumi Zuhanis Has-Yun Worthington, Jenny Allsopp, Philip Ternan, Nigel G. Brown, Emma M. McCann, Mark J. Rowland, Ian R. Esposto, Sonia Servili, Maurizio Gill, Chris I. R.
author_facet	Hashim, Yumi Zuhanis Has-Yun Worthington, Jenny Allsopp, Philip Ternan, Nigel G. Brown, Emma M. McCann, Mark J. Rowland, Ian R. Esposto, Sonia Servili, Maurizio Gill, Chris I. R.
author_sort	Hashim, Yumi Zuhanis Has-Yun
title	Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo
title_short	Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo
title_full	Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo
title_fullStr	Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo
title_full_unstemmed	Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo
title_sort	virgin olive oil phenolics extract inhibit invasion of ht115 human colon cancer cells in vitro and in vivo
publisher	The Royal Society of Chemist
publishDate	2014
url	http://irep.iium.edu.my/38022/ http://irep.iium.edu.my/38022/ http://irep.iium.edu.my/38022/ http://irep.iium.edu.my/38022/1/Paper_-_Hashim_et_al_2014_%28Food%26Func%29.pdf http://irep.iium.edu.my/38022/4/38022_Virgin%20olive%20oil_scopus.pdf
first_indexed	2023-09-18T20:54:33Z
last_indexed	2023-09-18T20:54:33Z
_version_	1777410216985362432

Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo

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