Virgin olive oil phenolics extract inhibit invasion of HT115 human colon cancer cells in vitro and in vivo
The decreased cancer risk associated with consumption of olive oilmay be due to the presence of phenolics which can modulate pathways including apoptosis and invasion that are relevant to carcinogenesis. We have previously shown that a virgin olive oil phenolics extract (OVP) inhibited invasion of...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
The Royal Society of Chemist
2014
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/38022/ http://irep.iium.edu.my/38022/ http://irep.iium.edu.my/38022/ http://irep.iium.edu.my/38022/1/Paper_-_Hashim_et_al_2014_%28Food%26Func%29.pdf http://irep.iium.edu.my/38022/4/38022_Virgin%20olive%20oil_scopus.pdf |
| Summary: | The decreased cancer risk associated with consumption of olive oilmay be due to the presence of phenolics
which can modulate pathways including apoptosis and invasion that are relevant to carcinogenesis. We have
previously shown that a virgin olive oil phenolics extract (OVP) inhibited invasion of HT115 colon cancer cells
in vitro. In the current study we assessed the in vitro effects of OVP (25 mg mL-1) on HT115 cell migration,
spreading and integrin expression. Furthermore, the anti-metastatic activity of OVP – at a dose equivalent
to 25 mg per kg per day for 2, 8 or 10 weeks – was assessed in a Severe Combined ImmunoDeficiency
(SCID) Balb-c mouse model. After 24 h OVP did not inhibit cell migration but significantly reduced cell
spreading on fibronectin (65% of control; p < 0.05) and expression of a range of a and b integrins was
modulated. In vivo, OVP by gavage significantly (p < 0.05) decreased not only tumour volume but also the
number of metastases in SCID Balb-c mice. Collectively, the data suggest that – possibly through
modulation of integrin expression – OVP decreases invasion in vitro and also inhibits metastasis in vivo. |
|---|