Alteration of Peripheral Blood Lymphocytes (PBLs) profile with HBsAg level in patients with chronic hepatitis B infection

Chronic hepatitis B infection is associated with dysfunction of cell- mediated immunity. Little is known about the changes of immune response during chronic hepatitis B infection, particularly in correlation between sequential alterations in peripheral blood immune cells population and hepatitis B s...

Full description

Bibliographic Details
Main Authors:	Hadi, Qabas Naemah, Mustafa Mahmoud, Mohammed Imad Al-Deen, Hui, Yee Chee, Jaafar, Khairul Azhar, Momtaz, Yadollah Abolfathi
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	QR355 Virology
Online Access:	http://irep.iium.edu.my/37876/ http://irep.iium.edu.my/37876/ http://irep.iium.edu.my/37876/1/C614057.pdf

id	iium-37876
recordtype	eprints
spelling	iium-378762015-03-26T02:24:43Z http://irep.iium.edu.my/37876/ Alteration of Peripheral Blood Lymphocytes (PBLs) profile with HBsAg level in patients with chronic hepatitis B infection Hadi, Qabas Naemah Mustafa Mahmoud, Mohammed Imad Al-Deen Hui, Yee Chee Jaafar, Khairul Azhar Momtaz, Yadollah Abolfathi QR355 Virology Chronic hepatitis B infection is associated with dysfunction of cell- mediated immunity. Little is known about the changes of immune response during chronic hepatitis B infection, particularly in correlation between sequential alterations in peripheral blood immune cells population and hepatitis B surface antigen (HBsAg). AIM: to examine the dynamic changes in the population of peripheral blood lymphocyte (PBL) subsets (T cells subsets, B lymphocytes, and NK cells) in healthy donors and patients with CHBI and their correlation with the level of HBsAg (qHBsAg). METHODS: the immunophenotype profiles of PBL of 50(HCV, HDV and HIV negative) chronic hepatitis B patients and 25 healthy controls were analyzed by Flowcytometry (FCM). In addition the serum HBeAg status was determined by ELISA and the HBsAg level was quantified by Elecsys assay (Roche Diagnostics, Germany). Results: significant reduction in both the percentages of CD4+(p≤0.05), CD8+ T lymphocyte subsets and the CD4+/ CD8+ ratio (p<0.01) was found in chronic hepatitis B patients as compared with the healthy donors. Meanwhile, there were no significant differences between patients and healthy controls with regard to other PBL parameters (total T, B, and NK cells), but a significant correlation was observed between HBsAg level and the percentages of T and NK cells (r=0.366; p<0.01, r=-0.462; p<0.05) respectively. Conclusion: our findings confirmed that CHB patients may have adversely affected cell-mediated immunity which is significantly correlated with a higher HBsAg level that leading to progress of the disease these patients. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/37876/1/C614057.pdf Hadi, Qabas Naemah and Mustafa Mahmoud, Mohammed Imad Al-Deen and Hui, Yee Chee and Jaafar, Khairul Azhar and Momtaz, Yadollah Abolfathi (2014) Alteration of Peripheral Blood Lymphocytes (PBLs) profile with HBsAg level in patients with chronic hepatitis B infection. In: International Conference on Biological, Chemical and Environmental Sciences (BCES-2014), 14-15 Jun 2014, Penang, Malaysia. http://iicbe.org/siteadmin/upload/7008C614057.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR355 Virology
spellingShingle	QR355 Virology Hadi, Qabas Naemah Mustafa Mahmoud, Mohammed Imad Al-Deen Hui, Yee Chee Jaafar, Khairul Azhar Momtaz, Yadollah Abolfathi Alteration of Peripheral Blood Lymphocytes (PBLs) profile with HBsAg level in patients with chronic hepatitis B infection
description	Chronic hepatitis B infection is associated with dysfunction of cell- mediated immunity. Little is known about the changes of immune response during chronic hepatitis B infection, particularly in correlation between sequential alterations in peripheral blood immune cells population and hepatitis B surface antigen (HBsAg). AIM: to examine the dynamic changes in the population of peripheral blood lymphocyte (PBL) subsets (T cells subsets, B lymphocytes, and NK cells) in healthy donors and patients with CHBI and their correlation with the level of HBsAg (qHBsAg). METHODS: the immunophenotype profiles of PBL of 50(HCV, HDV and HIV negative) chronic hepatitis B patients and 25 healthy controls were analyzed by Flowcytometry (FCM). In addition the serum HBeAg status was determined by ELISA and the HBsAg level was quantified by Elecsys assay (Roche Diagnostics, Germany). Results: significant reduction in both the percentages of CD4+(p≤0.05), CD8+ T lymphocyte subsets and the CD4+/ CD8+ ratio (p<0.01) was found in chronic hepatitis B patients as compared with the healthy donors. Meanwhile, there were no significant differences between patients and healthy controls with regard to other PBL parameters (total T, B, and NK cells), but a significant correlation was observed between HBsAg level and the percentages of T and NK cells (r=0.366; p<0.01, r=-0.462; p<0.05) respectively. Conclusion: our findings confirmed that CHB patients may have adversely affected cell-mediated immunity which is significantly correlated with a higher HBsAg level that leading to progress of the disease these patients.
format	Conference or Workshop Item
author	Hadi, Qabas Naemah Mustafa Mahmoud, Mohammed Imad Al-Deen Hui, Yee Chee Jaafar, Khairul Azhar Momtaz, Yadollah Abolfathi
author_facet	Hadi, Qabas Naemah Mustafa Mahmoud, Mohammed Imad Al-Deen Hui, Yee Chee Jaafar, Khairul Azhar Momtaz, Yadollah Abolfathi
author_sort	Hadi, Qabas Naemah
title	Alteration of Peripheral Blood Lymphocytes (PBLs) profile with HBsAg level in patients with chronic hepatitis B infection
title_short	Alteration of Peripheral Blood Lymphocytes (PBLs) profile with HBsAg level in patients with chronic hepatitis B infection
title_full	Alteration of Peripheral Blood Lymphocytes (PBLs) profile with HBsAg level in patients with chronic hepatitis B infection
title_fullStr	Alteration of Peripheral Blood Lymphocytes (PBLs) profile with HBsAg level in patients with chronic hepatitis B infection
title_full_unstemmed	Alteration of Peripheral Blood Lymphocytes (PBLs) profile with HBsAg level in patients with chronic hepatitis B infection
title_sort	alteration of peripheral blood lymphocytes (pbls) profile with hbsag level in patients with chronic hepatitis b infection
publishDate	2014
url	http://irep.iium.edu.my/37876/ http://irep.iium.edu.my/37876/ http://irep.iium.edu.my/37876/1/C614057.pdf
first_indexed	2023-09-18T20:54:18Z
last_indexed	2023-09-18T20:54:18Z
_version_	1777410201405620224

Alteration of Peripheral Blood Lymphocytes (PBLs) profile with HBsAg level in patients with chronic hepatitis B infection

Similar Items