Cytotoxic activity of luvunga scandens plant on human cancer cell lines

Luvunga scandens is a plant species belongs to the family of Rutaceae which come from the same family as citrus. Only a few studies on antifungal and insecticidal activities of L. scandens have been reported [1]. Yet, no study have reported the cytotoxic activity of this plant. Based on the traditi...

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Main Authors:	Al Zikri, Putri Nurhidayah, Bakhtiar, M. Taher, Darnis, Deny Susanti, Arief Ichwan, Solachuddin Jauhari
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/37837/ http://irep.iium.edu.my/37837/ http://irep.iium.edu.my/37837/1/ICIP_%281%29.pdf

id	iium-37837
recordtype	eprints
spelling	iium-378372018-06-11T05:56:07Z http://irep.iium.edu.my/37837/ Cytotoxic activity of luvunga scandens plant on human cancer cell lines Al Zikri, Putri Nurhidayah Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari RS Pharmacy and materia medica Luvunga scandens is a plant species belongs to the family of Rutaceae which come from the same family as citrus. Only a few studies on antifungal and insecticidal activities of L. scandens have been reported [1]. Yet, no study have reported the cytotoxic activity of this plant. Based on the traditional usage, stem of L. scandens via decoction was taken orally to treat malarial fever and tiredness [2;3]. Hence, this study is the first report on cytotoxic activity and phytochemical screening of the species of L. scandens. The aim of this study was to investigate the phytochemical and cytotoxic activity of the leaf and stem extract of L. scandens plant. Extracts of the leaf and stem were obtained from sequential extraction procedures by various organic solvents. All extracts were subjected to phytochemical screening to identify the chemical constituents and cytotoxic activities by 3-(4, 5-dimethylthaizol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay to determine the viability of cells. The result shows that the stem and leaf extracts of L. scandens were found to be responsible for the cytotoxic effects based on the dose-response inhibition. The IC50 value of extracts from leaf of L. scandens; LSC-LD and LSC-LM against MCF-7 cell line were 62.5 μg/mL and 80.0 μg/mL respectively. Whereas the IC50 value of extracts from stem of L. scandens; LSC-SD and LSC-SM were 75.0 μg/mL and 77.0 μg/mL respectively. For A549 cell line, all extracts exhibit dose-response inhibition but not able to reduce the percentage of cell viability to 50 %. So, the IC50 cannot be extrapolated. In conclusion, this present study demonstrates that all extracts of L. scandens exhibit cytotoxic activities with dose-response inhibition against MCF-7 and A549 cell lines. It therefore revealed that L. scandens is a potential source of cytotoxic agent. 2014-08-16 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/37837/1/ICIP_%281%29.pdf Al Zikri, Putri Nurhidayah and Bakhtiar, M. Taher and Darnis, Deny Susanti and Arief Ichwan, Solachuddin Jauhari (2014) Cytotoxic activity of luvunga scandens plant on human cancer cell lines. In: 1st International Conference on Industrial Pharmacy, 16-17 August 2014, Kuantan, Pahang. http://www.iium.edu.my/icip2014/download/proceeding.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Al Zikri, Putri Nurhidayah Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Cytotoxic activity of luvunga scandens plant on human cancer cell lines
description	Luvunga scandens is a plant species belongs to the family of Rutaceae which come from the same family as citrus. Only a few studies on antifungal and insecticidal activities of L. scandens have been reported [1]. Yet, no study have reported the cytotoxic activity of this plant. Based on the traditional usage, stem of L. scandens via decoction was taken orally to treat malarial fever and tiredness [2;3]. Hence, this study is the first report on cytotoxic activity and phytochemical screening of the species of L. scandens. The aim of this study was to investigate the phytochemical and cytotoxic activity of the leaf and stem extract of L. scandens plant. Extracts of the leaf and stem were obtained from sequential extraction procedures by various organic solvents. All extracts were subjected to phytochemical screening to identify the chemical constituents and cytotoxic activities by 3-(4, 5-dimethylthaizol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay to determine the viability of cells. The result shows that the stem and leaf extracts of L. scandens were found to be responsible for the cytotoxic effects based on the dose-response inhibition. The IC50 value of extracts from leaf of L. scandens; LSC-LD and LSC-LM against MCF-7 cell line were 62.5 μg/mL and 80.0 μg/mL respectively. Whereas the IC50 value of extracts from stem of L. scandens; LSC-SD and LSC-SM were 75.0 μg/mL and 77.0 μg/mL respectively. For A549 cell line, all extracts exhibit dose-response inhibition but not able to reduce the percentage of cell viability to 50 %. So, the IC50 cannot be extrapolated. In conclusion, this present study demonstrates that all extracts of L. scandens exhibit cytotoxic activities with dose-response inhibition against MCF-7 and A549 cell lines. It therefore revealed that L. scandens is a potential source of cytotoxic agent.
format	Conference or Workshop Item
author	Al Zikri, Putri Nurhidayah Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari
author_facet	Al Zikri, Putri Nurhidayah Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari
author_sort	Al Zikri, Putri Nurhidayah
title	Cytotoxic activity of luvunga scandens plant on human cancer cell lines
title_short	Cytotoxic activity of luvunga scandens plant on human cancer cell lines
title_full	Cytotoxic activity of luvunga scandens plant on human cancer cell lines
title_fullStr	Cytotoxic activity of luvunga scandens plant on human cancer cell lines
title_full_unstemmed	Cytotoxic activity of luvunga scandens plant on human cancer cell lines
title_sort	cytotoxic activity of luvunga scandens plant on human cancer cell lines
publishDate	2014
url	http://irep.iium.edu.my/37837/ http://irep.iium.edu.my/37837/ http://irep.iium.edu.my/37837/1/ICIP_%281%29.pdf
first_indexed	2023-09-18T20:54:15Z
last_indexed	2023-09-18T20:54:15Z
_version_	1777410197581463552

Cytotoxic activity of luvunga scandens plant on human cancer cell lines

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