Identification of putative Cof-like hydrolase associated with dehalogenase in Enterobacter cloacae MN1 isolated from the contaminated sea-side area of the Philippines

Aims: The present study aimed at molecular identification of putative Cof-like hydrolase associated with dehalogenase gene from a bacterium that was isolated from a contaminated sea-side area in the Philippines. The bacterium was subjected to 16S rRNA gene sequence analysis for identity of the genus...

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Main Authors:	Abdul Hamid, Azzmer Azzar, Nemati, Mahdieh, Abdulghader, Mohamed Faraj, Gicana, Ronie G, Ibtrahim, Nurhafizah, Huyop, Fahrul Zaman
Format:	Article
Language:	English
Published:	Malaysian Society for Microbiology 2013
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/37515/ http://irep.iium.edu.my/37515/ http://irep.iium.edu.my/37515/1/Nemati_et_al._%282013%29.pdf

id	iium-37515
recordtype	eprints
spelling	iium-375152014-08-13T02:07:40Z http://irep.iium.edu.my/37515/ Identification of putative Cof-like hydrolase associated with dehalogenase in Enterobacter cloacae MN1 isolated from the contaminated sea-side area of the Philippines Abdul Hamid, Azzmer Azzar Nemati, Mahdieh Abdulghader, Mohamed Faraj Gicana, Ronie G Ibtrahim, Nurhafizah Huyop, Fahrul Zaman TP248.13 Biotechnology Aims: The present study aimed at molecular identification of putative Cof-like hydrolase associated with dehalogenase gene from a bacterium that was isolated from a contaminated sea-side area in the Philippines. The bacterium was subjected to 16S rRNA gene sequence analysis for identity of the genus and species. Methodology and results: Based on basic microbiological analysis and 16S rRNA sequence determination, strain MN1 showed high sequence identity to Enterobacter cloacae. This is the first reported study that Enterobacter could degrade 2,2-dichloropropionate (2,2-DCP). A putative dehalogenase gene like was identified by direct sequencing and analysis of the PCR-amplified genomic DNA of the bacterium. A comparative analysis of the sequence data revealed that the amino acid sequence is closely related to several Cof-like hydrolase associated with L-specific dehalogenases. Conclusion, significance and impact of study: Current study may suggest that the hydrolase may have similar function to dehalogenase. However, further analysis like enzyme assay need to be carried out to confirm this. Putative dehalogenase gene can be amplified using PCR technique provided that the specific primers designed were used. Malaysian Society for Microbiology 2013-04-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/37515/1/Nemati_et_al._%282013%29.pdf Abdul Hamid, Azzmer Azzar and Nemati, Mahdieh and Abdulghader, Mohamed Faraj and Gicana, Ronie G and Ibtrahim, Nurhafizah and Huyop, Fahrul Zaman (2013) Identification of putative Cof-like hydrolase associated with dehalogenase in Enterobacter cloacae MN1 isolated from the contaminated sea-side area of the Philippines. Malaysian Journal of Microbiology, 9 (3). pp. 253-259. ISSN 1823-8262 http://web.usm.my/mjm/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Abdul Hamid, Azzmer Azzar Nemati, Mahdieh Abdulghader, Mohamed Faraj Gicana, Ronie G Ibtrahim, Nurhafizah Huyop, Fahrul Zaman Identification of putative Cof-like hydrolase associated with dehalogenase in Enterobacter cloacae MN1 isolated from the contaminated sea-side area of the Philippines
description	Aims: The present study aimed at molecular identification of putative Cof-like hydrolase associated with dehalogenase gene from a bacterium that was isolated from a contaminated sea-side area in the Philippines. The bacterium was subjected to 16S rRNA gene sequence analysis for identity of the genus and species. Methodology and results: Based on basic microbiological analysis and 16S rRNA sequence determination, strain MN1 showed high sequence identity to Enterobacter cloacae. This is the first reported study that Enterobacter could degrade 2,2-dichloropropionate (2,2-DCP). A putative dehalogenase gene like was identified by direct sequencing and analysis of the PCR-amplified genomic DNA of the bacterium. A comparative analysis of the sequence data revealed that the amino acid sequence is closely related to several Cof-like hydrolase associated with L-specific dehalogenases. Conclusion, significance and impact of study: Current study may suggest that the hydrolase may have similar function to dehalogenase. However, further analysis like enzyme assay need to be carried out to confirm this. Putative dehalogenase gene can be amplified using PCR technique provided that the specific primers designed were used.
format	Article
author	Abdul Hamid, Azzmer Azzar Nemati, Mahdieh Abdulghader, Mohamed Faraj Gicana, Ronie G Ibtrahim, Nurhafizah Huyop, Fahrul Zaman
author_facet	Abdul Hamid, Azzmer Azzar Nemati, Mahdieh Abdulghader, Mohamed Faraj Gicana, Ronie G Ibtrahim, Nurhafizah Huyop, Fahrul Zaman
author_sort	Abdul Hamid, Azzmer Azzar
title	Identification of putative Cof-like hydrolase associated with dehalogenase in Enterobacter cloacae MN1 isolated from the contaminated sea-side area of the Philippines
title_short	Identification of putative Cof-like hydrolase associated with dehalogenase in Enterobacter cloacae MN1 isolated from the contaminated sea-side area of the Philippines
title_full	Identification of putative Cof-like hydrolase associated with dehalogenase in Enterobacter cloacae MN1 isolated from the contaminated sea-side area of the Philippines
title_fullStr	Identification of putative Cof-like hydrolase associated with dehalogenase in Enterobacter cloacae MN1 isolated from the contaminated sea-side area of the Philippines
title_full_unstemmed	Identification of putative Cof-like hydrolase associated with dehalogenase in Enterobacter cloacae MN1 isolated from the contaminated sea-side area of the Philippines
title_sort	identification of putative cof-like hydrolase associated with dehalogenase in enterobacter cloacae mn1 isolated from the contaminated sea-side area of the philippines
publisher	Malaysian Society for Microbiology
publishDate	2013
url	http://irep.iium.edu.my/37515/ http://irep.iium.edu.my/37515/ http://irep.iium.edu.my/37515/1/Nemati_et_al._%282013%29.pdf
first_indexed	2023-09-18T20:53:49Z
last_indexed	2023-09-18T20:53:49Z
_version_	1777410170927710208

Identification of putative Cof-like hydrolase associated with dehalogenase in Enterobacter cloacae MN1 isolated from the contaminated sea-side area of the Philippines

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