HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger

The study was carried out to develop a rapid and accurate HPLC method to isolate the major bioactive phytochemical asiaticoside in Centella asiatica and to transform this metabolite using microorganism. Asiaticoside was separated using an optimized HPLC method using Phenomenex, Luna, 5 μm (C18) (150...

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Main Authors:	Alfarra, Helmi Yousif, Omar, Muhammad Nor
Format:	Article
Language:	English
Published:	Engineering and Technology Publishing 2014
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/37448/ http://irep.iium.edu.my/37448/ http://irep.iium.edu.my/37448/1/IJPMBS_DR_nO.pdf

id	iium-37448
recordtype	eprints
spelling	iium-374482018-06-19T07:34:05Z http://irep.iium.edu.my/37448/ HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger Alfarra, Helmi Yousif Omar, Muhammad Nor QD Chemistry The study was carried out to develop a rapid and accurate HPLC method to isolate the major bioactive phytochemical asiaticoside in Centella asiatica and to transform this metabolite using microorganism. Asiaticoside was separated using an optimized HPLC method using Phenomenex, Luna, 5 μm (C18) (150 mm x 4.6 mm) eluted with Water PH3: Acetonitrile (50:50 V/V). The reported method is easy, fast and accurate, and produced highly pure, resolved isolate with high concentration. Asiaticoside was biologically transformed by Aspergillus niger, and the prescreening results on TLC and 1D and 2D IR showed that the transformed produced has at least two byproducts of the ancestor compound. In addition to that the preliminary screeing showed that the transformed product showed very high cytotoxicity agains HaCat Cells in comprison to the original compound. Engineering and Technology Publishing 2014-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/37448/1/IJPMBS_DR_nO.pdf Alfarra, Helmi Yousif and Omar, Muhammad Nor (2014) HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger. International Journal of Pharma Medicine and Biological Sciences, 3 (3). pp. 1-8. ISSN 2278-5221 http://www.ijpmbs.com/ijpmbsadmin/upload/ijpmbs_53ba6734e5a0f.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Alfarra, Helmi Yousif Omar, Muhammad Nor HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger
description	The study was carried out to develop a rapid and accurate HPLC method to isolate the major bioactive phytochemical asiaticoside in Centella asiatica and to transform this metabolite using microorganism. Asiaticoside was separated using an optimized HPLC method using Phenomenex, Luna, 5 μm (C18) (150 mm x 4.6 mm) eluted with Water PH3: Acetonitrile (50:50 V/V). The reported method is easy, fast and accurate, and produced highly pure, resolved isolate with high concentration. Asiaticoside was biologically transformed by Aspergillus niger, and the prescreening results on TLC and 1D and 2D IR showed that the transformed produced has at least two byproducts of the ancestor compound. In addition to that the preliminary screeing showed that the transformed product showed very high cytotoxicity agains HaCat Cells in comprison to the original compound.
format	Article
author	Alfarra, Helmi Yousif Omar, Muhammad Nor
author_facet	Alfarra, Helmi Yousif Omar, Muhammad Nor
author_sort	Alfarra, Helmi Yousif
title	HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger
title_short	HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger
title_full	HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger
title_fullStr	HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger
title_full_unstemmed	HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger
title_sort	hplc separation and isolation of asiaticoside from centella asiatica and its biotransformation by a. niger
publisher	Engineering and Technology Publishing
publishDate	2014
url	http://irep.iium.edu.my/37448/ http://irep.iium.edu.my/37448/ http://irep.iium.edu.my/37448/1/IJPMBS_DR_nO.pdf
first_indexed	2023-09-18T20:53:44Z
last_indexed	2023-09-18T20:53:44Z
_version_	1777410165059878912

HPLC separation and isolation of asiaticoside from Centella asiatica and its biotransformation by A. niger

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