Suitability of using palm oil mill effluent as a medium for lipase production

Suitability of using palm oil mill effluent as a medium for lipase production

Lipases are enzymes that can be secreted by several microorganisms using agro-industrial residues as potential substrates. This work screened ten microorganisms for their potential to produce lipase in palm oil mill effluent (POME)-based medium. Among the 10 organisms, the most promising strain wa...

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Main Authors: Salihu, Aliyu, Alam, Md Zahangir, AbdulKarim, M. Ismail, Mohd. Salleh, Hamzah
Format: Article
Language:English
Published: Academic Journals 2011
Subjects:
QD Chemistry
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/3741/
http://irep.iium.edu.my/3741/
http://irep.iium.edu.my/3741/1/Salihu_et_al_2011-AJB.pdf
id iium-3741
recordtype eprints
spelling iium-37412017-08-10T03:24:48Z http://irep.iium.edu.my/3741/ Suitability of using palm oil mill effluent as a medium for lipase production Salihu, Aliyu Alam, Md Zahangir AbdulKarim, M. Ismail Mohd. Salleh, Hamzah QD Chemistry TP248.13 Biotechnology Lipases are enzymes that can be secreted by several microorganisms using agro-industrial residues as potential substrates. This work screened ten microorganisms for their potential to produce lipase in palm oil mill effluent (POME)-based medium. Among the 10 organisms, the most promising strain was Candida cylindracea (ATCC 14830) which showed appreciable activity both on agar plates and liquid cultures. Medium supplementation by NH4Cl and olive oil led to an enzyme activity of 2.07 U/ml. However, supplementation with organic nitrogen sources resulted in better enzyme activity. Addition of malt extract, peptone and olive oil into the medium greatly influenced the lipase production. Among the oils that were tested, olive oil was found to be the best for the expression of extracellular lipase at 0.5% (v/v) with an activity of 4.02 U/ml in an optimized POME supplemented medium. Academic Journals 2011-03-14 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3741/1/Salihu_et_al_2011-AJB.pdf Salihu, Aliyu and Alam, Md Zahangir and AbdulKarim, M. Ismail and Mohd. Salleh, Hamzah (2011) Suitability of using palm oil mill effluent as a medium for lipase production. African Journal of Biotechnology, 10 (11). pp. 2044-2052. ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/14Mar/Salihu%20et%20al.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
TP248.13 Biotechnology
spellingShingle QD Chemistry
TP248.13 Biotechnology
Salihu, Aliyu
Alam, Md Zahangir
AbdulKarim, M. Ismail
Mohd. Salleh, Hamzah
Suitability of using palm oil mill effluent as a medium for lipase production
description Lipases are enzymes that can be secreted by several microorganisms using agro-industrial residues as potential substrates. This work screened ten microorganisms for their potential to produce lipase in palm oil mill effluent (POME)-based medium. Among the 10 organisms, the most promising strain was Candida cylindracea (ATCC 14830) which showed appreciable activity both on agar plates and liquid cultures. Medium supplementation by NH4Cl and olive oil led to an enzyme activity of 2.07 U/ml. However, supplementation with organic nitrogen sources resulted in better enzyme activity. Addition of malt extract, peptone and olive oil into the medium greatly influenced the lipase production. Among the oils that were tested, olive oil was found to be the best for the expression of extracellular lipase at 0.5% (v/v) with an activity of 4.02 U/ml in an optimized POME supplemented medium.
format Article
author Salihu, Aliyu
Alam, Md Zahangir
AbdulKarim, M. Ismail
Mohd. Salleh, Hamzah
author_facet Salihu, Aliyu
Alam, Md Zahangir
AbdulKarim, M. Ismail
Mohd. Salleh, Hamzah
author_sort Salihu, Aliyu
title Suitability of using palm oil mill effluent as a medium for lipase production
title_short Suitability of using palm oil mill effluent as a medium for lipase production
title_full Suitability of using palm oil mill effluent as a medium for lipase production
title_fullStr Suitability of using palm oil mill effluent as a medium for lipase production
title_full_unstemmed Suitability of using palm oil mill effluent as a medium for lipase production
title_sort suitability of using palm oil mill effluent as a medium for lipase production
publisher Academic Journals
publishDate 2011
url http://irep.iium.edu.my/3741/
http://irep.iium.edu.my/3741/
http://irep.iium.edu.my/3741/1/Salihu_et_al_2011-AJB.pdf
first_indexed 2023-09-18T20:11:42Z
last_indexed 2023-09-18T20:11:42Z
_version_ 1777407520533381120

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