Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium

Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium

A two-level full factorial design (FFD) was employed to determine the effects of process parameters on lipase production by Candida cylindracea ATCC 14830 in palm oil mill effluent (POME)-based medium. Ten experimental runs based on three parameters (temperature, agitation and aeration) as indicate...

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Main Authors: Salihu, Aliyu, Alam, Md Zahangir, AbdulKarim, M. Ismail, Mohd. Salleh, Hamzah
Format: Article
Language:English
Published: Elsevier B.V. 2011
Subjects:
QD Chemistry
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/3736/
http://irep.iium.edu.my/3736/
http://irep.iium.edu.my/3736/
http://irep.iium.edu.my/3736/1/MOLCAB_process_optimization_article.pdf
id iium-3736
recordtype eprints
spelling iium-37362017-08-10T02:44:58Z http://irep.iium.edu.my/3736/ Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium Salihu, Aliyu Alam, Md Zahangir AbdulKarim, M. Ismail Mohd. Salleh, Hamzah QD Chemistry TP248.13 Biotechnology A two-level full factorial design (FFD) was employed to determine the effects of process parameters on lipase production by Candida cylindracea ATCC 14830 in palm oil mill effluent (POME)-based medium. Ten experimental runs based on three parameters (temperature, agitation and aeration) as indicated by the FFD were carried out in a stirred-tank bioreactor. On statistical analysis of the results, the optimum temperature, aeration and agitation rates were found to be 30 ◦C, 1.0 vvm and 400 rpm respectively, with a maximum activity of 41.46 U/ml after 36 h of fermentation. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) value of 0.999, indicating a satisfactory fit of the model with the experimental data. All the three parameters were statistically significant at p < 0.05. The validation experiment also confirmed that apart from lipase production, there was an increase in chemical oxygen demand (COD) removal throughout the fermentation period. Elsevier B.V. 2011-06-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3736/1/MOLCAB_process_optimization_article.pdf Salihu, Aliyu and Alam, Md Zahangir and AbdulKarim, M. Ismail and Mohd. Salleh, Hamzah (2011) Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium. Journal of Molecular Catalysis B: Enzymatic, 72 ( ). pp. 187-192. ISSN 1381-1177 http://dx.doi.org/10.1016/j.molcatb.2011.06.004 doi:10.1016/j.molcatb.2011.06.004
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
TP248.13 Biotechnology
spellingShingle QD Chemistry
TP248.13 Biotechnology
Salihu, Aliyu
Alam, Md Zahangir
AbdulKarim, M. Ismail
Mohd. Salleh, Hamzah
Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium
description A two-level full factorial design (FFD) was employed to determine the effects of process parameters on lipase production by Candida cylindracea ATCC 14830 in palm oil mill effluent (POME)-based medium. Ten experimental runs based on three parameters (temperature, agitation and aeration) as indicated by the FFD were carried out in a stirred-tank bioreactor. On statistical analysis of the results, the optimum temperature, aeration and agitation rates were found to be 30 ◦C, 1.0 vvm and 400 rpm respectively, with a maximum activity of 41.46 U/ml after 36 h of fermentation. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) value of 0.999, indicating a satisfactory fit of the model with the experimental data. All the three parameters were statistically significant at p < 0.05. The validation experiment also confirmed that apart from lipase production, there was an increase in chemical oxygen demand (COD) removal throughout the fermentation period.
format Article
author Salihu, Aliyu
Alam, Md Zahangir
AbdulKarim, M. Ismail
Mohd. Salleh, Hamzah
author_facet Salihu, Aliyu
Alam, Md Zahangir
AbdulKarim, M. Ismail
Mohd. Salleh, Hamzah
author_sort Salihu, Aliyu
title Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium
title_short Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium
title_full Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium
title_fullStr Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium
title_full_unstemmed Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium
title_sort effect of process parameters on lipase production by candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium
publisher Elsevier B.V.
publishDate 2011
url http://irep.iium.edu.my/3736/
http://irep.iium.edu.my/3736/
http://irep.iium.edu.my/3736/
http://irep.iium.edu.my/3736/1/MOLCAB_process_optimization_article.pdf
first_indexed 2023-09-18T20:11:41Z
last_indexed 2023-09-18T20:11:41Z
_version_ 1777407519825592320

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