Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bio-active constituents in the crude extract

Waterlily Mango (Mangifera indica L.) is thought to be antioxidant-rich, conferred by its functional hytochemicals. Methods: The potential anticancer effects of the ethanolic kernel extract on breast cancer cells (MDAMB-231 and MCF-7) using MTT, anti-proliferation, neutral red (NR) uptake and lac...

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Main Authors:	Hussah Abdullah, Al-Shwyeh, Sabo Muhammed, Abdulkarim, Abdullah, Rasadee, Mirghani, Mohamed Elwathig Saeed, Al-Qubaisi, Mothanna
Format:	Article
Language:	English English
Published:	BioMed Central 2014
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/37324/ http://irep.iium.edu.my/37324/ http://irep.iium.edu.my/37324/ http://irep.iium.edu.my/37324/1/BMC_published_article_2004_%28Hussah%29.pdf http://irep.iium.edu.my/37324/4/37324_Cytotoxic%20effects%20of%20Mangifera_SCOPUS.pdf

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spelling	iium-373242017-09-19T07:22:14Z http://irep.iium.edu.my/37324/ Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bio-active constituents in the crude extract Hussah Abdullah, Al-Shwyeh Sabo Muhammed, Abdulkarim Abdullah, Rasadee Mirghani, Mohamed Elwathig Saeed Al-Qubaisi, Mothanna TP248.13 Biotechnology Waterlily Mango (Mangifera indica L.) is thought to be antioxidant-rich, conferred by its functional hytochemicals. Methods: The potential anticancer effects of the ethanolic kernel extract on breast cancer cells (MDAMB-231 and MCF-7) using MTT, anti-proliferation, neutral red (NR) uptake and lactate dehydrogenase (LDH) release assays were evaluated. Cytological studies on the breast cancer cells were also conducted, and phytochemical analyses of the extract were carried out to determine the likely bioactive compounds responsible for such effects. Results: Results showed the extract induced cytotoxicity in MDA-MB-231 cells and MCF-7 cells with IC50 values of 30 and 15 μg/mL, respectively. The extract showed significant toxicity towards both cell lines, with low toxicity to normal breast cells (MCF-10A). The cytotoxic effects on the cells were further confirmed by the NR uptake, antiproliferative and LDH release assays. Bioactive analyses revealed that many bioactives were present in the extract although butylated hydroxytoluene, a potent antioxidant, was the most abundant with 44.65%. Conclusions: M. indica extract appears to be more cytoxic to both estrogen positive and negative breast cancer cell lines than to normal breast cells. Synergistic effects of its ntioxidant bioactives could have contributed to the cytotoxic effects of the extract. The extract of M. indica, therefore, has potential anticancer activity against breast cancer cells. This potential is worth studying further, and could have implications on future studies and eventually management of human breast cancers. BioMed Central 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/37324/1/BMC_published_article_2004_%28Hussah%29.pdf application/pdf en http://irep.iium.edu.my/37324/4/37324_Cytotoxic%20effects%20of%20Mangifera_SCOPUS.pdf Hussah Abdullah, Al-Shwyeh and Sabo Muhammed, Abdulkarim and Abdullah, Rasadee and Mirghani, Mohamed Elwathig Saeed and Al-Qubaisi, Mothanna (2014) Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bio-active constituents in the crude extract. BMC Complementary and Alternative Medicine, 14 (199). pp. 1-15. ISSN 1472-6882 (In Press) http://www.biomedcentral.com/1472-6882/14/199 10.1186/1472-6882-14-199
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Hussah Abdullah, Al-Shwyeh Sabo Muhammed, Abdulkarim Abdullah, Rasadee Mirghani, Mohamed Elwathig Saeed Al-Qubaisi, Mothanna Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bio-active constituents in the crude extract
description	Waterlily Mango (Mangifera indica L.) is thought to be antioxidant-rich, conferred by its functional hytochemicals. Methods: The potential anticancer effects of the ethanolic kernel extract on breast cancer cells (MDAMB-231 and MCF-7) using MTT, anti-proliferation, neutral red (NR) uptake and lactate dehydrogenase (LDH) release assays were evaluated. Cytological studies on the breast cancer cells were also conducted, and phytochemical analyses of the extract were carried out to determine the likely bioactive compounds responsible for such effects. Results: Results showed the extract induced cytotoxicity in MDA-MB-231 cells and MCF-7 cells with IC50 values of 30 and 15 μg/mL, respectively. The extract showed significant toxicity towards both cell lines, with low toxicity to normal breast cells (MCF-10A). The cytotoxic effects on the cells were further confirmed by the NR uptake, antiproliferative and LDH release assays. Bioactive analyses revealed that many bioactives were present in the extract although butylated hydroxytoluene, a potent antioxidant, was the most abundant with 44.65%. Conclusions: M. indica extract appears to be more cytoxic to both estrogen positive and negative breast cancer cell lines than to normal breast cells. Synergistic effects of its ntioxidant bioactives could have contributed to the cytotoxic effects of the extract. The extract of M. indica, therefore, has potential anticancer activity against breast cancer cells. This potential is worth studying further, and could have implications on future studies and eventually management of human breast cancers.
format	Article
author	Hussah Abdullah, Al-Shwyeh Sabo Muhammed, Abdulkarim Abdullah, Rasadee Mirghani, Mohamed Elwathig Saeed Al-Qubaisi, Mothanna
author_facet	Hussah Abdullah, Al-Shwyeh Sabo Muhammed, Abdulkarim Abdullah, Rasadee Mirghani, Mohamed Elwathig Saeed Al-Qubaisi, Mothanna
author_sort	Hussah Abdullah, Al-Shwyeh
title	Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bio-active constituents in the crude extract
title_short	Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bio-active constituents in the crude extract
title_full	Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bio-active constituents in the crude extract
title_fullStr	Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bio-active constituents in the crude extract
title_full_unstemmed	Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bio-active constituents in the crude extract
title_sort	cytotoxic effects of mangifera indica l. kernel extract on human breast cancer (mcf-7 and mda-mb-231 cell lines) and bio-active constituents in the crude extract
publisher	BioMed Central
publishDate	2014
url	http://irep.iium.edu.my/37324/ http://irep.iium.edu.my/37324/ http://irep.iium.edu.my/37324/ http://irep.iium.edu.my/37324/1/BMC_published_article_2004_%28Hussah%29.pdf http://irep.iium.edu.my/37324/4/37324_Cytotoxic%20effects%20of%20Mangifera_SCOPUS.pdf
first_indexed	2023-09-18T20:53:33Z
last_indexed	2023-09-18T20:53:33Z
_version_	1777410154042490880

Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA-MB-231 cell lines) and bio-active constituents in the crude extract

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