Molecular characterization of monochloroacetate-degrading arthrobacter sp. Strain d2 isolated from Universiti Teknologi Malaysia agricultural area

Arthrobacter sp. strains D2 and D3 and Labrys sp. strain D1 capable of degrading 20 mM monochloroacetic acid (MCA) were isolated from soil contaminated with herbicides and pesticides. All three isolates were able to grow onMCAas the sole source of carbon and energy with concomitant chloride ion rel...

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Main Authors:	Alomar, Duha, Abdul Hamid, Azzmer Azzar, Khosrowabadi, Elham, Gicana, Ronnie G., Lamis, Robert J., Huyop, Fahrul Zaman, Tengku Abdul Hamid, Tengku Haziyamin
Format:	Article
Language:	English English English
Published:	Taylor & Francis 2014
Subjects:	QR Microbiology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/37213/ http://irep.iium.edu.my/37213/ http://irep.iium.edu.my/37213/ http://irep.iium.edu.my/37213/1/Alomar_et_al._%282014%29.pdf http://irep.iium.edu.my/37213/4/37213_Molecular%20characterization%20of%20monochloroacetate-degrading_SCOPUS.pdf http://irep.iium.edu.my/37213/5/37213_Molecular%20characterization%20of%20monochloroacetate-degrading_WoS.pdf

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spelling	iium-372132017-09-25T09:58:26Z http://irep.iium.edu.my/37213/ Molecular characterization of monochloroacetate-degrading arthrobacter sp. Strain d2 isolated from Universiti Teknologi Malaysia agricultural area Alomar, Duha Abdul Hamid, Azzmer Azzar Khosrowabadi, Elham Gicana, Ronnie G. Lamis, Robert J. Huyop, Fahrul Zaman Tengku Abdul Hamid, Tengku Haziyamin QR Microbiology TP248.13 Biotechnology Arthrobacter sp. strains D2 and D3 and Labrys sp. strain D1 capable of degrading 20 mM monochloroacetic acid (MCA) were isolated from soil contaminated with herbicides and pesticides. All three isolates were able to grow onMCAas the sole source of carbon and energy with concomitant chloride ion release in the growth medium (19 mM). Strains D2 and D3 (cells doubling time 7±0.3 h) grew four times faster than D1 (26±0.1 h). Strain D2 was then further investigated and could also grow in 10 mM of monobromoacetic acid (MBA),2,2-dichloropropionic acid (2,2DCP), D,L-2-chloropropionic acid (D,L2CP), L- 2-chloropropionic acid (L-2CP), D-2-chloropropionic acid (D-2CP), and glycolate as the sole sources of carbon and energy. Dehalogenase gene amplification using group I primers revealed a 410-bp polymerase chain reaction (PCR) product, but there was none using group II primers. The partial amino acid sequence analysis of group I DehD2 dehalogenase showed at least 32% identity to the corresponding regions of DehE, DhlIV, DehI, and D,L-DEX, with key amino acid residues Ser188, Ala187, and Asp189. These amino acid residues were involved in substrate binding and catalysis and were conserved in the partial amino acid sequence. Taylor & Francis 2014-01-26 Article PeerReviewed application/pdf en http://irep.iium.edu.my/37213/1/Alomar_et_al._%282014%29.pdf application/pdf en http://irep.iium.edu.my/37213/4/37213_Molecular%20characterization%20of%20monochloroacetate-degrading_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/37213/5/37213_Molecular%20characterization%20of%20monochloroacetate-degrading_WoS.pdf Alomar, Duha and Abdul Hamid, Azzmer Azzar and Khosrowabadi, Elham and Gicana, Ronnie G. and Lamis, Robert J. and Huyop, Fahrul Zaman and Tengku Abdul Hamid, Tengku Haziyamin (2014) Molecular characterization of monochloroacetate-degrading arthrobacter sp. Strain d2 isolated from Universiti Teknologi Malaysia agricultural area. Bioremediation Journal, 18 (1). pp. 12-19. ISSN 1088-9868 http://www.tandfonline.com/ 10.1080/10889868.2013.834867
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QR Microbiology TP248.13 Biotechnology
spellingShingle	QR Microbiology TP248.13 Biotechnology Alomar, Duha Abdul Hamid, Azzmer Azzar Khosrowabadi, Elham Gicana, Ronnie G. Lamis, Robert J. Huyop, Fahrul Zaman Tengku Abdul Hamid, Tengku Haziyamin Molecular characterization of monochloroacetate-degrading arthrobacter sp. Strain d2 isolated from Universiti Teknologi Malaysia agricultural area
description	Arthrobacter sp. strains D2 and D3 and Labrys sp. strain D1 capable of degrading 20 mM monochloroacetic acid (MCA) were isolated from soil contaminated with herbicides and pesticides. All three isolates were able to grow onMCAas the sole source of carbon and energy with concomitant chloride ion release in the growth medium (19 mM). Strains D2 and D3 (cells doubling time 7±0.3 h) grew four times faster than D1 (26±0.1 h). Strain D2 was then further investigated and could also grow in 10 mM of monobromoacetic acid (MBA),2,2-dichloropropionic acid (2,2DCP), D,L-2-chloropropionic acid (D,L2CP), L- 2-chloropropionic acid (L-2CP), D-2-chloropropionic acid (D-2CP), and glycolate as the sole sources of carbon and energy. Dehalogenase gene amplification using group I primers revealed a 410-bp polymerase chain reaction (PCR) product, but there was none using group II primers. The partial amino acid sequence analysis of group I DehD2 dehalogenase showed at least 32% identity to the corresponding regions of DehE, DhlIV, DehI, and D,L-DEX, with key amino acid residues Ser188, Ala187, and Asp189. These amino acid residues were involved in substrate binding and catalysis and were conserved in the partial amino acid sequence.
format	Article
author	Alomar, Duha Abdul Hamid, Azzmer Azzar Khosrowabadi, Elham Gicana, Ronnie G. Lamis, Robert J. Huyop, Fahrul Zaman Tengku Abdul Hamid, Tengku Haziyamin
author_facet	Alomar, Duha Abdul Hamid, Azzmer Azzar Khosrowabadi, Elham Gicana, Ronnie G. Lamis, Robert J. Huyop, Fahrul Zaman Tengku Abdul Hamid, Tengku Haziyamin
author_sort	Alomar, Duha
title	Molecular characterization of monochloroacetate-degrading arthrobacter sp. Strain d2 isolated from Universiti Teknologi Malaysia agricultural area
title_short	Molecular characterization of monochloroacetate-degrading arthrobacter sp. Strain d2 isolated from Universiti Teknologi Malaysia agricultural area
title_full	Molecular characterization of monochloroacetate-degrading arthrobacter sp. Strain d2 isolated from Universiti Teknologi Malaysia agricultural area
title_fullStr	Molecular characterization of monochloroacetate-degrading arthrobacter sp. Strain d2 isolated from Universiti Teknologi Malaysia agricultural area
title_full_unstemmed	Molecular characterization of monochloroacetate-degrading arthrobacter sp. Strain d2 isolated from Universiti Teknologi Malaysia agricultural area
title_sort	molecular characterization of monochloroacetate-degrading arthrobacter sp. strain d2 isolated from universiti teknologi malaysia agricultural area
publisher	Taylor & Francis
publishDate	2014
url	http://irep.iium.edu.my/37213/ http://irep.iium.edu.my/37213/ http://irep.iium.edu.my/37213/ http://irep.iium.edu.my/37213/1/Alomar_et_al._%282014%29.pdf http://irep.iium.edu.my/37213/4/37213_Molecular%20characterization%20of%20monochloroacetate-degrading_SCOPUS.pdf http://irep.iium.edu.my/37213/5/37213_Molecular%20characterization%20of%20monochloroacetate-degrading_WoS.pdf
first_indexed	2023-09-18T20:53:22Z
last_indexed	2023-09-18T20:53:22Z
_version_	1777410142665441280

Molecular characterization of monochloroacetate-degrading arthrobacter sp. Strain d2 isolated from Universiti Teknologi Malaysia agricultural area

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