The growth study of vero cells in different type of microcarrier

The fact of microcarrier (MC) culture introduces new possibilities and makes possible the practical high-yield culture of anchorage-dependent cells has generated a considerable focus in this study. The objective of this research was to study the comparison of Vero cell growth on different types of...

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Main Authors:	Ahmad Nor, Yusilawati, Sulong, Nurul Hafizah, Mel, Maizirwan, Mohd. Salleh, Hamzah, Sopyan, Iis
Format:	Article
Language:	English
Published:	Scientific Research 2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/3721/ http://irep.iium.edu.my/3721/ http://irep.iium.edu.my/3721/ http://irep.iium.edu.my/3721/1/MSA-Microcarrier_2010.pdf

id	iium-3721
recordtype	eprints
spelling	iium-37212011-11-16T02:07:26Z http://irep.iium.edu.my/3721/ The growth study of vero cells in different type of microcarrier Ahmad Nor, Yusilawati Sulong, Nurul Hafizah Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis TP248.13 Biotechnology The fact of microcarrier (MC) culture introduces new possibilities and makes possible the practical high-yield culture of anchorage-dependent cells has generated a considerable focus in this study. The objective of this research was to study the comparison of Vero cell growth on different types of commercial microcarriers; Cytodex-1, Cytodex-3, Hillex® II and Plastic Plus in spinner vessel and two liters bioreactor cultured for 96 hours. Biological performance of the microcarrier in RPMI media showed the preference of Vero cell grew on Cytodex 3 microcarriers with highest maximum viable cell number (2.4 × 105 cells/ml) followed by Cytodex 1, Hillex and Plustic Plus. Vero cell on Cytodex- 3 data in spinner flask was compared in bioreactor and result showed higher viable cell number in biorector. Thus, this dextran-crosslink gelatin microcarrier (Cytodex 3) provided the best surface for cell attachment and fast proliferation. At the end of this cell growth improvement will be used for virus transfection producing a vaccine in bioreactor. Scientific Research 2010-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3721/1/MSA-Microcarrier_2010.pdf Ahmad Nor, Yusilawati and Sulong, Nurul Hafizah and Mel, Maizirwan and Mohd. Salleh, Hamzah and Sopyan, Iis (2010) The growth study of vero cells in different type of microcarrier. Materials Sciences and Applications, 1 (5). pp. 261-266. ISSN 2153-1188 (O), 2153-117X (P) http://www.scirp.org/journal/PaperInformation.aspx?paperID=3286 doi:10.4236/msa.2010.15038
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ahmad Nor, Yusilawati Sulong, Nurul Hafizah Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis The growth study of vero cells in different type of microcarrier
description	The fact of microcarrier (MC) culture introduces new possibilities and makes possible the practical high-yield culture of anchorage-dependent cells has generated a considerable focus in this study. The objective of this research was to study the comparison of Vero cell growth on different types of commercial microcarriers; Cytodex-1, Cytodex-3, Hillex® II and Plastic Plus in spinner vessel and two liters bioreactor cultured for 96 hours. Biological performance of the microcarrier in RPMI media showed the preference of Vero cell grew on Cytodex 3 microcarriers with highest maximum viable cell number (2.4 × 105 cells/ml) followed by Cytodex 1, Hillex and Plustic Plus. Vero cell on Cytodex- 3 data in spinner flask was compared in bioreactor and result showed higher viable cell number in biorector. Thus, this dextran-crosslink gelatin microcarrier (Cytodex 3) provided the best surface for cell attachment and fast proliferation. At the end of this cell growth improvement will be used for virus transfection producing a vaccine in bioreactor.
format	Article
author	Ahmad Nor, Yusilawati Sulong, Nurul Hafizah Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis
author_facet	Ahmad Nor, Yusilawati Sulong, Nurul Hafizah Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis
author_sort	Ahmad Nor, Yusilawati
title	The growth study of vero cells in different type of microcarrier
title_short	The growth study of vero cells in different type of microcarrier
title_full	The growth study of vero cells in different type of microcarrier
title_fullStr	The growth study of vero cells in different type of microcarrier
title_full_unstemmed	The growth study of vero cells in different type of microcarrier
title_sort	growth study of vero cells in different type of microcarrier
publisher	Scientific Research
publishDate	2010
url	http://irep.iium.edu.my/3721/ http://irep.iium.edu.my/3721/ http://irep.iium.edu.my/3721/ http://irep.iium.edu.my/3721/1/MSA-Microcarrier_2010.pdf
first_indexed	2023-09-18T20:11:40Z
last_indexed	2023-09-18T20:11:40Z
_version_	1777407518502289408

The growth study of vero cells in different type of microcarrier

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