Proteomic analysis and pharmacological activities of the venom of the Moroccan cobra Naja haje legionis

Background: In Morocco, envenomation by snake bites poses a serious problem to public health. The cobra Naja haje legionis is endemic of the country and one of the most dangerous species known. In this work, we report the proteomic and pharmacological characterizations of biologically active pro...

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Main Authors:	Malih, Ibitissam, Ahmad Rusmili, Muhamad Rusdi, Ting, Yee Tee, Saile, Rachid, Ghalim, Noreddine, Othman, Iekhsan
Format:	Article
Language:	English
Published:	Elsevier 2012
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/37205/ http://irep.iium.edu.my/37205/ http://irep.iium.edu.my/37205/ http://irep.iium.edu.my/37205/1/rusdi_proteomic.pdf

id	iium-37205
recordtype	eprints
spelling	iium-372052014-07-09T05:07:55Z http://irep.iium.edu.my/37205/ Proteomic analysis and pharmacological activities of the venom of the Moroccan cobra Naja haje legionis Malih, Ibitissam Ahmad Rusmili, Muhamad Rusdi Ting, Yee Tee Saile, Rachid Ghalim, Noreddine Othman, Iekhsan RM Therapeutics. Pharmacology Background: In Morocco, envenomation by snake bites poses a serious problem to public health. The cobra Naja haje legionis is endemic of the country and one of the most dangerous species known. In this work, we report the proteomic and pharmacological characterizations of biologically active proteins from the venom of this species. Methods: The various proteins in the crude venom were fractionated and analyzed by a combination of chromatographic separations and proteomic analysis techniques such as gel filtration, RP-HPLC, 1D electrophoresis, in-gel digestion, tandem mass spectrometry and protein database search. The pharmacological properties of Naja haje legionis venom were assessed using in vitro preparations using rodents and chicks. Results: Our venomic strategy allowed the identification of 64 proteins and peptides from known database which can classified into 17 families according to their biological activities. We were able to identify cobra venom factor, L-aminoacid oxidases, acetylcholinesterase, metalloproteinase, disintegrin, cysteine-rich secretory proteins, nerve growth factor, phospholipases A2, vespryns, kunitz-type inhibitor, short neurotoxins, long neurotoxins, weak neurotoxins, neurotoxin like proteins, muscarinic toxins, cytotoxins and cardiotoxins. Pharmacological tests showed that Naja haje legionis venom contained neurotoxic activities inducing irreversible blockage of neuromuscular transmission in both rodent and chick nerve-muscle preparations. This venom also exhibit myotoxic and cardiotoxic activities. Conclusions: These results showed the potency and the complexity of the various proteins present in the venom of Naja haje legionis,which present a very similar pattern to other cobra venoms. The contribution of the different components in venom toxicity deserves further investigations. Elsevier 2012 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/37205/1/rusdi_proteomic.pdf Malih, Ibitissam and Ahmad Rusmili, Muhamad Rusdi and Ting, Yee Tee and Saile, Rachid and Ghalim, Noreddine and Othman, Iekhsan (2012) Proteomic analysis and pharmacological activities of the venom of the Moroccan cobra Naja haje legionis. Toxicon, 60 (2). p. 211. ISSN 0041-0101 http://www.sciencedirect.com/science/article/pii/S0041010112003224 10.1016/j.toxicon.2012.04.226
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Malih, Ibitissam Ahmad Rusmili, Muhamad Rusdi Ting, Yee Tee Saile, Rachid Ghalim, Noreddine Othman, Iekhsan Proteomic analysis and pharmacological activities of the venom of the Moroccan cobra Naja haje legionis
description	Background: In Morocco, envenomation by snake bites poses a serious problem to public health. The cobra Naja haje legionis is endemic of the country and one of the most dangerous species known. In this work, we report the proteomic and pharmacological characterizations of biologically active proteins from the venom of this species. Methods: The various proteins in the crude venom were fractionated and analyzed by a combination of chromatographic separations and proteomic analysis techniques such as gel filtration, RP-HPLC, 1D electrophoresis, in-gel digestion, tandem mass spectrometry and protein database search. The pharmacological properties of Naja haje legionis venom were assessed using in vitro preparations using rodents and chicks. Results: Our venomic strategy allowed the identification of 64 proteins and peptides from known database which can classified into 17 families according to their biological activities. We were able to identify cobra venom factor, L-aminoacid oxidases, acetylcholinesterase, metalloproteinase, disintegrin, cysteine-rich secretory proteins, nerve growth factor, phospholipases A2, vespryns, kunitz-type inhibitor, short neurotoxins, long neurotoxins, weak neurotoxins, neurotoxin like proteins, muscarinic toxins, cytotoxins and cardiotoxins. Pharmacological tests showed that Naja haje legionis venom contained neurotoxic activities inducing irreversible blockage of neuromuscular transmission in both rodent and chick nerve-muscle preparations. This venom also exhibit myotoxic and cardiotoxic activities. Conclusions: These results showed the potency and the complexity of the various proteins present in the venom of Naja haje legionis,which present a very similar pattern to other cobra venoms. The contribution of the different components in venom toxicity deserves further investigations.
format	Article
author	Malih, Ibitissam Ahmad Rusmili, Muhamad Rusdi Ting, Yee Tee Saile, Rachid Ghalim, Noreddine Othman, Iekhsan
author_facet	Malih, Ibitissam Ahmad Rusmili, Muhamad Rusdi Ting, Yee Tee Saile, Rachid Ghalim, Noreddine Othman, Iekhsan
author_sort	Malih, Ibitissam
title	Proteomic analysis and pharmacological activities of the venom of the Moroccan cobra Naja haje legionis
title_short	Proteomic analysis and pharmacological activities of the venom of the Moroccan cobra Naja haje legionis
title_full	Proteomic analysis and pharmacological activities of the venom of the Moroccan cobra Naja haje legionis
title_fullStr	Proteomic analysis and pharmacological activities of the venom of the Moroccan cobra Naja haje legionis
title_full_unstemmed	Proteomic analysis and pharmacological activities of the venom of the Moroccan cobra Naja haje legionis
title_sort	proteomic analysis and pharmacological activities of the venom of the moroccan cobra naja haje legionis
publisher	Elsevier
publishDate	2012
url	http://irep.iium.edu.my/37205/ http://irep.iium.edu.my/37205/ http://irep.iium.edu.my/37205/ http://irep.iium.edu.my/37205/1/rusdi_proteomic.pdf
first_indexed	2023-09-18T20:53:22Z
last_indexed	2023-09-18T20:53:22Z
_version_	1777410141951361024

Proteomic analysis and pharmacological activities of the venom of the Moroccan cobra Naja haje legionis

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