Proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry

The proteome of the venom of Naja haje legionis, the only medically important elapid species in Morocco, has been elucidated by using a combination of proteomic techniques that includes size exclusion chromatography, reverse-phase HPLC, Tricine/SDS-Page, tryptic digestion, Q-TOF tandem mass spectrom...

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Main Authors:	Malih, Ibitissam, Ahmad Rusmili, Muhamad Rusdi, Ting, Yee Tee, Saile, Rachid, Ghalim, Noreddine, Othman, Iekhsan
Format:	Article
Language:	English
Published:	Elsevier Ltd. 2014
Subjects:	QH301 Biology QL Zoology RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/37199/ http://irep.iium.edu.my/37199/ http://irep.iium.edu.my/37199/ http://irep.iium.edu.my/37199/1/1-s2.0-S1874391913006106-main.pdf

id	iium-37199
recordtype	eprints
spelling	iium-371992018-06-20T07:40:42Z http://irep.iium.edu.my/37199/ Proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry Malih, Ibitissam Ahmad Rusmili, Muhamad Rusdi Ting, Yee Tee Saile, Rachid Ghalim, Noreddine Othman, Iekhsan QH301 Biology QL Zoology RM Therapeutics. Pharmacology The proteome of the venom of Naja haje legionis, the only medically important elapid species in Morocco, has been elucidated by using a combination of proteomic techniques that includes size exclusion chromatography, reverse-phase HPLC, Tricine/SDS-Page, tryptic digestion, Q-TOF tandem mass spectrometry and database search. The sequence analysis of venom fractions revealed a highly complex venom proteome which counts a total of 76 proteins identified from database that can be assigned into 9 proteins families. We report the identification of: cobra venom factor (CVF), l-amino-acid oxidases (LAAO), acetylcholinesterase (AChE), snake venom metalloproteinases (SVMP), cysteine rich secretory proteins (CRISP), venom nerve growth factor (vNGF), phospholipases A2 (PLA2), vespryns, kunitz-type inhibitor, short neurotoxins, long neurotoxins, weak neurotoxins, neurotoxin like proteins, muscarinic toxins, cardiotoxins and cytotoxins. Comparison of these proteins showed high sequence homology with proteins from other African and Asian cobras. Further works are needed to assess the contribution of individual toxins in venom toxicity. Elsevier Ltd. 2014-01-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/37199/1/1-s2.0-S1874391913006106-main.pdf Malih, Ibitissam and Ahmad Rusmili, Muhamad Rusdi and Ting, Yee Tee and Saile, Rachid and Ghalim, Noreddine and Othman, Iekhsan (2014) Proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry. Journal of Proteomics, 96. pp. 240-252. ISSN 1874-3919 http://www.sciencedirect.com/science/article/pii/S1874391913006106 10.1016/j.jprot.2013.11.012
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QH301 Biology QL Zoology RM Therapeutics. Pharmacology
spellingShingle	QH301 Biology QL Zoology RM Therapeutics. Pharmacology Malih, Ibitissam Ahmad Rusmili, Muhamad Rusdi Ting, Yee Tee Saile, Rachid Ghalim, Noreddine Othman, Iekhsan Proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry
description	The proteome of the venom of Naja haje legionis, the only medically important elapid species in Morocco, has been elucidated by using a combination of proteomic techniques that includes size exclusion chromatography, reverse-phase HPLC, Tricine/SDS-Page, tryptic digestion, Q-TOF tandem mass spectrometry and database search. The sequence analysis of venom fractions revealed a highly complex venom proteome which counts a total of 76 proteins identified from database that can be assigned into 9 proteins families. We report the identification of: cobra venom factor (CVF), l-amino-acid oxidases (LAAO), acetylcholinesterase (AChE), snake venom metalloproteinases (SVMP), cysteine rich secretory proteins (CRISP), venom nerve growth factor (vNGF), phospholipases A2 (PLA2), vespryns, kunitz-type inhibitor, short neurotoxins, long neurotoxins, weak neurotoxins, neurotoxin like proteins, muscarinic toxins, cardiotoxins and cytotoxins. Comparison of these proteins showed high sequence homology with proteins from other African and Asian cobras. Further works are needed to assess the contribution of individual toxins in venom toxicity.
format	Article
author	Malih, Ibitissam Ahmad Rusmili, Muhamad Rusdi Ting, Yee Tee Saile, Rachid Ghalim, Noreddine Othman, Iekhsan
author_facet	Malih, Ibitissam Ahmad Rusmili, Muhamad Rusdi Ting, Yee Tee Saile, Rachid Ghalim, Noreddine Othman, Iekhsan
author_sort	Malih, Ibitissam
title	Proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry
title_short	Proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry
title_full	Proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry
title_fullStr	Proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry
title_full_unstemmed	Proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry
title_sort	proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry
publisher	Elsevier Ltd.
publishDate	2014
url	http://irep.iium.edu.my/37199/ http://irep.iium.edu.my/37199/ http://irep.iium.edu.my/37199/ http://irep.iium.edu.my/37199/1/1-s2.0-S1874391913006106-main.pdf
first_indexed	2023-09-18T20:53:21Z
last_indexed	2023-09-18T20:53:21Z
_version_	1777410141470064640

Proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry

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