Optimization of spray drying process conditions for recombinant stem bromelain

Bromelain is a plant protease with numerous therapeutic, industrial and analytical applications. Spray drying of enzyme often leads to loss of enzymatic activity arising from thermal denaturation. Hence, the design of a suitable drying process should provide a great level of active enzyme. The pr...

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Main Authors:	Mohd. Salleh, Hamzah, Mel, Maizirwan, Jami, Mohammed Saedi, Amid, Azura, Muntari, Bala
Format:	Article
Language:	English English
Published:	AENSI Publications 2014
Subjects:	TP155 Chemical engineering
Online Access:	http://irep.iium.edu.my/37154/ http://irep.iium.edu.my/37154/ http://irep.iium.edu.my/37154/1/696-703.pdf http://irep.iium.edu.my/37154/4/37154_Optimization%20of%20spray%20drying%20process.SCOPUS.pdf

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spelling	iium-371542018-06-19T08:30:53Z http://irep.iium.edu.my/37154/ Optimization of spray drying process conditions for recombinant stem bromelain Mohd. Salleh, Hamzah Mel, Maizirwan Jami, Mohammed Saedi Amid, Azura Muntari, Bala TP155 Chemical engineering Bromelain is a plant protease with numerous therapeutic, industrial and analytical applications. Spray drying of enzyme often leads to loss of enzymatic activity arising from thermal denaturation. Hence, the design of a suitable drying process should provide a great level of active enzyme. The present study examined the effect of operating parameters of a laboratory spray dryer on powder characteristics, so as to optimize the production of recombinant bromelain expressed in E. coli BL 21-AI. The recombinant enzyme was spray dried from maltodextrin (10 %w/v), CaCl2 (0.2 % w/v) and sodium metabisulphite (2.5 % w/v) solutions using a laboratory-scale Büchi Mini Spray dryer B-290. The process parameters investigated were: drying air inlet temperature (100-120 ºC), drying air volumetric flow rate – given as % of the maximum aspiration rate (80-100 %), feed volumetric flow rate– expressed as % of the maximum pump rate (10-15 %). On the other hand, the activity of bromelain (U/ml) served as the response of the design. Outlet temperature was maintained at 50 oC. All the studied process parameters had significantly affected the characteristics of the powdered bromelain, at a 95% confidence interval. The higher values of coefficient of determination (R2 = 99.95% and adjusted R2 = 99.84%) attained, showed that there is good compliance between the experimental and the theoretical values predicted by the model. Moreover, the graphical representations of the regression equation generated suggested that the examined independent variables interacted significantly. Thus, the corroboration of reality of the optimal conditions and the validity of the model had been ascertained. Consequently, under optimized process conditions, the study had produced powdered bromelain with greater quality in terms of moisture contents, residual activity and product recovery. AENSI Publications 2014-03-25 Article PeerReviewed application/pdf en http://irep.iium.edu.my/37154/1/696-703.pdf application/pdf en http://irep.iium.edu.my/37154/4/37154_Optimization%20of%20spray%20drying%20process.SCOPUS.pdf Mohd. Salleh, Hamzah and Mel, Maizirwan and Jami, Mohammed Saedi and Amid, Azura and Muntari, Bala (2014) Optimization of spray drying process conditions for recombinant stem bromelain. Advances in Environmental Biology, 8 (3). pp. 696-703. ISSN 1998-1066 (O), 1995-0756 (P) http://www.aensiweb.com/old/aeb/2014/696-703.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP155 Chemical engineering
spellingShingle	TP155 Chemical engineering Mohd. Salleh, Hamzah Mel, Maizirwan Jami, Mohammed Saedi Amid, Azura Muntari, Bala Optimization of spray drying process conditions for recombinant stem bromelain
description	Bromelain is a plant protease with numerous therapeutic, industrial and analytical applications. Spray drying of enzyme often leads to loss of enzymatic activity arising from thermal denaturation. Hence, the design of a suitable drying process should provide a great level of active enzyme. The present study examined the effect of operating parameters of a laboratory spray dryer on powder characteristics, so as to optimize the production of recombinant bromelain expressed in E. coli BL 21-AI. The recombinant enzyme was spray dried from maltodextrin (10 %w/v), CaCl2 (0.2 % w/v) and sodium metabisulphite (2.5 % w/v) solutions using a laboratory-scale Büchi Mini Spray dryer B-290. The process parameters investigated were: drying air inlet temperature (100-120 ºC), drying air volumetric flow rate – given as % of the maximum aspiration rate (80-100 %), feed volumetric flow rate– expressed as % of the maximum pump rate (10-15 %). On the other hand, the activity of bromelain (U/ml) served as the response of the design. Outlet temperature was maintained at 50 oC. All the studied process parameters had significantly affected the characteristics of the powdered bromelain, at a 95% confidence interval. The higher values of coefficient of determination (R2 = 99.95% and adjusted R2 = 99.84%) attained, showed that there is good compliance between the experimental and the theoretical values predicted by the model. Moreover, the graphical representations of the regression equation generated suggested that the examined independent variables interacted significantly. Thus, the corroboration of reality of the optimal conditions and the validity of the model had been ascertained. Consequently, under optimized process conditions, the study had produced powdered bromelain with greater quality in terms of moisture contents, residual activity and product recovery.
format	Article
author	Mohd. Salleh, Hamzah Mel, Maizirwan Jami, Mohammed Saedi Amid, Azura Muntari, Bala
author_facet	Mohd. Salleh, Hamzah Mel, Maizirwan Jami, Mohammed Saedi Amid, Azura Muntari, Bala
author_sort	Mohd. Salleh, Hamzah
title	Optimization of spray drying process conditions for recombinant stem bromelain
title_short	Optimization of spray drying process conditions for recombinant stem bromelain
title_full	Optimization of spray drying process conditions for recombinant stem bromelain
title_fullStr	Optimization of spray drying process conditions for recombinant stem bromelain
title_full_unstemmed	Optimization of spray drying process conditions for recombinant stem bromelain
title_sort	optimization of spray drying process conditions for recombinant stem bromelain
publisher	AENSI Publications
publishDate	2014
url	http://irep.iium.edu.my/37154/ http://irep.iium.edu.my/37154/ http://irep.iium.edu.my/37154/1/696-703.pdf http://irep.iium.edu.my/37154/4/37154_Optimization%20of%20spray%20drying%20process.SCOPUS.pdf
first_indexed	2023-09-18T20:53:18Z
last_indexed	2023-09-18T20:53:18Z
_version_	1777410137999278080

Optimization of spray drying process conditions for recombinant stem bromelain

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