Optimization of spray drying process conditions for recombinant stem bromelain
Bromelain is a plant protease with numerous therapeutic, industrial and analytical applications. Spray drying of enzyme often leads to loss of enzymatic activity arising from thermal denaturation. Hence, the design of a suitable drying process should provide a great level of active enzyme. The pr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
AENSI Publications
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/37154/ http://irep.iium.edu.my/37154/ http://irep.iium.edu.my/37154/1/696-703.pdf http://irep.iium.edu.my/37154/4/37154_Optimization%20of%20spray%20drying%20process.SCOPUS.pdf |
| Summary: | Bromelain is a plant protease with numerous therapeutic, industrial and analytical
applications. Spray drying of enzyme often leads to loss of enzymatic activity arising
from thermal denaturation. Hence, the design of a suitable drying process should
provide a great level of active enzyme. The present study examined the effect of
operating parameters of a laboratory spray dryer on powder characteristics, so as to
optimize the production of recombinant bromelain expressed in E. coli BL 21-AI. The
recombinant enzyme was spray dried from maltodextrin (10 %w/v), CaCl2 (0.2 % w/v)
and sodium metabisulphite (2.5 % w/v) solutions using a laboratory-scale Büchi Mini
Spray dryer B-290. The process parameters investigated were: drying air inlet
temperature (100-120 ºC), drying air volumetric flow rate – given as % of the
maximum aspiration rate (80-100 %), feed volumetric flow rate– expressed as % of the
maximum pump rate (10-15 %). On the other hand, the activity of bromelain (U/ml)
served as the response of the design. Outlet temperature was maintained at 50 oC. All
the studied process parameters had significantly affected the characteristics of the
powdered bromelain, at a 95% confidence interval. The higher values of coefficient of
determination (R2 = 99.95% and adjusted R2 = 99.84%) attained, showed that there is
good compliance between the experimental and the theoretical values predicted by the
model. Moreover, the graphical representations of the regression equation generated
suggested that the examined independent variables interacted significantly. Thus, the
corroboration of reality of the optimal conditions and the validity of the model had been
ascertained. Consequently, under optimized process conditions, the study had produced
powdered bromelain with greater quality in terms of moisture contents, residual activity
and product recovery. |
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