Antioxidant and antimicrobial activities of shorea kunstleri

Objective: To evaluate antioxidant and antimicrobial activities of stembark of Shorea kunstleri together with analysis of phytochemical and total phenolic contents. Methods: Extraction was conducted with different solvent polarity of n-hexane, dichloromethane (DCM) and methanol by using Soxhlet e...

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Main Authors:	Daud, Siti Suria, Bakhtiar, M. Taher, Darnis, Deny Susanti, Mohamed Zaffar Ali, Mohamed Amiroudine, Tengku Zakaria, Tengku Muhamad Faris Syafiq
Format:	Article
Language:	English
Published:	Coastal Life Medicine Media co., Limited 2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/37028/ http://irep.iium.edu.my/37028/ http://irep.iium.edu.my/37028/1/JCLM_Shorea.pdf

id	iium-37028
recordtype	eprints
spelling	iium-370282017-06-20T06:37:48Z http://irep.iium.edu.my/37028/ Antioxidant and antimicrobial activities of shorea kunstleri Daud, Siti Suria Bakhtiar, M. Taher Darnis, Deny Susanti Mohamed Zaffar Ali, Mohamed Amiroudine Tengku Zakaria, Tengku Muhamad Faris Syafiq RS Pharmacy and materia medica Objective: To evaluate antioxidant and antimicrobial activities of stembark of Shorea kunstleri together with analysis of phytochemical and total phenolic contents. Methods: Extraction was conducted with different solvent polarity of n-hexane, dichloromethane (DCM) and methanol by using Soxhlet extraction. Total phenolic content was determined using Folin-Ciocalteu method. Free radical scavenging activity and inhibition of lipid peroxidation were evaluated with DPPH radical scavenging and ferric thiocyanate (FTC) assays, respectively. Antimicrobial activities were performed using disc diffusion method, minimum inhibition concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC). Results: S. kunstleri stembark extracts revealed presence of steroids, terpenoids, saponins, flavonoids, and phenolic compounds. Methanol extract exhibited the highest total phenolic content and free radical scavenging activity result in phenolic content of 8.34±0.003 g GAE/100 g of extract and 95.9±1.07% DPPH inhibition (IC50 value of 18.6 µg/ml), respectively. FTC assay of n-hexane, DCM, and methanol extracts indicated lipid peroxidation inhibitory activity of 74.2±0.35%, 74.0±0.10%, and 72.8±0.27%, respectively. In antimicrobial and antifungal tests, methanol extract showed inhibition against S. aureus, C. albicans, and C. tropicalis with inhibition zones of 10-12, 18-22, and 18-19 mm, respectively. The MIC test of methanol extract showed highest inhibition against C. albicans and S. aureus (0.04 and 0.08 mg/ml, respectively) while DCM extract exhibited the highest activity towards C. tropicalis (MIC value of 0.63 mg/ml). Taken together, MBC test of methanol extract strongly demonstrated bactericidal effect against S. aureus with MBC value of 0.08 mg/ml. Conclusion: The study demonstrated that stembark extracts of S. kunstleri possessed antioxidant and antimicrobial properties. Coastal Life Medicine Media co., Limited 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/37028/1/JCLM_Shorea.pdf Daud, Siti Suria and Bakhtiar, M. Taher and Darnis, Deny Susanti and Mohamed Zaffar Ali, Mohamed Amiroudine and Tengku Zakaria, Tengku Muhamad Faris Syafiq (2014) Antioxidant and antimicrobial activities of shorea kunstleri. Journal of Coastal Life Medicine, 2 (8). pp. 638-644. ISSN 2309-5288 E-ISSN 2309-6152 http://jclmm.com/zz/20148.htm
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Daud, Siti Suria Bakhtiar, M. Taher Darnis, Deny Susanti Mohamed Zaffar Ali, Mohamed Amiroudine Tengku Zakaria, Tengku Muhamad Faris Syafiq Antioxidant and antimicrobial activities of shorea kunstleri
description	Objective: To evaluate antioxidant and antimicrobial activities of stembark of Shorea kunstleri together with analysis of phytochemical and total phenolic contents. Methods: Extraction was conducted with different solvent polarity of n-hexane, dichloromethane (DCM) and methanol by using Soxhlet extraction. Total phenolic content was determined using Folin-Ciocalteu method. Free radical scavenging activity and inhibition of lipid peroxidation were evaluated with DPPH radical scavenging and ferric thiocyanate (FTC) assays, respectively. Antimicrobial activities were performed using disc diffusion method, minimum inhibition concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC). Results: S. kunstleri stembark extracts revealed presence of steroids, terpenoids, saponins, flavonoids, and phenolic compounds. Methanol extract exhibited the highest total phenolic content and free radical scavenging activity result in phenolic content of 8.34±0.003 g GAE/100 g of extract and 95.9±1.07% DPPH inhibition (IC50 value of 18.6 µg/ml), respectively. FTC assay of n-hexane, DCM, and methanol extracts indicated lipid peroxidation inhibitory activity of 74.2±0.35%, 74.0±0.10%, and 72.8±0.27%, respectively. In antimicrobial and antifungal tests, methanol extract showed inhibition against S. aureus, C. albicans, and C. tropicalis with inhibition zones of 10-12, 18-22, and 18-19 mm, respectively. The MIC test of methanol extract showed highest inhibition against C. albicans and S. aureus (0.04 and 0.08 mg/ml, respectively) while DCM extract exhibited the highest activity towards C. tropicalis (MIC value of 0.63 mg/ml). Taken together, MBC test of methanol extract strongly demonstrated bactericidal effect against S. aureus with MBC value of 0.08 mg/ml. Conclusion: The study demonstrated that stembark extracts of S. kunstleri possessed antioxidant and antimicrobial properties.
format	Article
author	Daud, Siti Suria Bakhtiar, M. Taher Darnis, Deny Susanti Mohamed Zaffar Ali, Mohamed Amiroudine Tengku Zakaria, Tengku Muhamad Faris Syafiq
author_facet	Daud, Siti Suria Bakhtiar, M. Taher Darnis, Deny Susanti Mohamed Zaffar Ali, Mohamed Amiroudine Tengku Zakaria, Tengku Muhamad Faris Syafiq
author_sort	Daud, Siti Suria
title	Antioxidant and antimicrobial activities of shorea kunstleri
title_short	Antioxidant and antimicrobial activities of shorea kunstleri
title_full	Antioxidant and antimicrobial activities of shorea kunstleri
title_fullStr	Antioxidant and antimicrobial activities of shorea kunstleri
title_full_unstemmed	Antioxidant and antimicrobial activities of shorea kunstleri
title_sort	antioxidant and antimicrobial activities of shorea kunstleri
publisher	Coastal Life Medicine Media co., Limited
publishDate	2014
url	http://irep.iium.edu.my/37028/ http://irep.iium.edu.my/37028/ http://irep.iium.edu.my/37028/1/JCLM_Shorea.pdf
first_indexed	2023-09-18T20:53:06Z
last_indexed	2023-09-18T20:53:06Z
_version_	1777410125897662464

Antioxidant and antimicrobial activities of shorea kunstleri

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