Study on callogenesis and growth kinetic of F. deltoidea Var. Trengganuensis cell suspension culture
Active compounds such as secondary metabolite from plants can be produced via its regeneration of organ or callus or from cell suspension culture. Thus, the aim of this study is to establish the protocol for callus regeneration and cell suspension culture of Ficusdeltoidea var. trengganuensis. Ca...
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| Format: | Article |
| Language: | English English |
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Journal of Pure and Applied Microbiology
2013
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| Online Access: | http://irep.iium.edu.my/36941/ http://irep.iium.edu.my/36941/ http://irep.iium.edu.my/36941/1/Tajul_work-JPAM07SplEditionP349-354.pdf http://irep.iium.edu.my/36941/4/36941_Cell%20suspension_scopus.pdf |
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iium-369412017-09-19T08:54:02Z http://irep.iium.edu.my/36941/ Study on callogenesis and growth kinetic of F. deltoidea Var. Trengganuensis cell suspension culture Abdullah, Tajul Afif Amid, Azura Mohamad Puad, Noor Illi Jamal, Parveen Jaafar, Hafsah TP248.13 Biotechnology Active compounds such as secondary metabolite from plants can be produced via its regeneration of organ or callus or from cell suspension culture. Thus, the aim of this study is to establish the protocol for callus regeneration and cell suspension culture of Ficusdeltoidea var. trengganuensis. Callus regeneration study was conducted on solid media consisted of 9 treatments with combination of picloram and 2,4-D ranging from 1.5ppm to 4.5ppm. After 4 weeks, the callus were weight under sterilize condition. Treatment consisted of MS+3ppm picloram+ 3ppm 2,4-D was found to form the highest weight of callus formation (68.8 ± 21.25mg). Cell suspension wasthen established where 4 weeks old of soft and friable callus of F.deltoidea were used as an inoculum at 2%(w/v) in a 100mL of media. About1ml media was collected at 5 days interval to determine its dry cell weight. The cell suspension culture established in this study showed an increase in its dry cell weight (mg/mL) until day 15th where the density of the biomass were observed to decrease at day 20th. The highest specific growth rate (5.49 x 10-3h-1)was observed at day 5 to day 10. Journal of Pure and Applied Microbiology 2013-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/36941/1/Tajul_work-JPAM07SplEditionP349-354.pdf application/pdf en http://irep.iium.edu.my/36941/4/36941_Cell%20suspension_scopus.pdf Abdullah, Tajul Afif and Amid, Azura and Mohamad Puad, Noor Illi and Jamal, Parveen and Jaafar, Hafsah (2013) Study on callogenesis and growth kinetic of F. deltoidea Var. Trengganuensis cell suspension culture. Journal of Pure and Applied Microbiology, 7 Spec. pp. 349-354. ISSN 0973-7510 http://www.microbiologyjournal.org/jmabsread.php?snoid=1363&month=&year= |
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Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
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Online Access |
| language |
English English |
| topic |
TP248.13 Biotechnology |
| spellingShingle |
TP248.13 Biotechnology Abdullah, Tajul Afif Amid, Azura Mohamad Puad, Noor Illi Jamal, Parveen Jaafar, Hafsah Study on callogenesis and growth kinetic of F. deltoidea Var. Trengganuensis cell suspension culture |
| description |
Active compounds such as secondary metabolite from plants can be produced
via its regeneration of organ or callus or from cell suspension culture. Thus, the aim of
this study is to establish the protocol for callus regeneration and cell suspension culture
of Ficusdeltoidea var. trengganuensis. Callus regeneration study was conducted on solid
media consisted of 9 treatments with combination of picloram and 2,4-D ranging from
1.5ppm to 4.5ppm. After 4 weeks, the callus were weight under sterilize condition.
Treatment consisted of MS+3ppm picloram+ 3ppm 2,4-D was found to form the highest
weight of callus formation (68.8 ± 21.25mg). Cell suspension wasthen established where
4 weeks old of soft and friable callus of F.deltoidea were used as an inoculum at 2%(w/v)
in a 100mL of media. About1ml media was collected at 5 days interval to determine its
dry cell weight. The cell suspension culture established in this study showed an increase
in its dry cell weight (mg/mL) until day 15th where the density of the biomass were
observed to decrease at day 20th. The highest specific growth rate (5.49 x 10-3h-1)was
observed at day 5 to day 10. |
| format |
Article |
| author |
Abdullah, Tajul Afif Amid, Azura Mohamad Puad, Noor Illi Jamal, Parveen Jaafar, Hafsah |
| author_facet |
Abdullah, Tajul Afif Amid, Azura Mohamad Puad, Noor Illi Jamal, Parveen Jaafar, Hafsah |
| author_sort |
Abdullah, Tajul Afif |
| title |
Study on callogenesis and growth kinetic of F. deltoidea Var.
Trengganuensis cell suspension culture |
| title_short |
Study on callogenesis and growth kinetic of F. deltoidea Var.
Trengganuensis cell suspension culture |
| title_full |
Study on callogenesis and growth kinetic of F. deltoidea Var.
Trengganuensis cell suspension culture |
| title_fullStr |
Study on callogenesis and growth kinetic of F. deltoidea Var.
Trengganuensis cell suspension culture |
| title_full_unstemmed |
Study on callogenesis and growth kinetic of F. deltoidea Var.
Trengganuensis cell suspension culture |
| title_sort |
study on callogenesis and growth kinetic of f. deltoidea var.
trengganuensis cell suspension culture |
| publisher |
Journal of Pure and Applied Microbiology |
| publishDate |
2013 |
| url |
http://irep.iium.edu.my/36941/ http://irep.iium.edu.my/36941/ http://irep.iium.edu.my/36941/1/Tajul_work-JPAM07SplEditionP349-354.pdf http://irep.iium.edu.my/36941/4/36941_Cell%20suspension_scopus.pdf |
| first_indexed |
2023-09-18T20:52:59Z |
| last_indexed |
2023-09-18T20:52:59Z |
| _version_ |
1777410118458015744 |