Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage

ARID3A/DRIL1/Bright is a family member of AT rich interaction domain (ARID) DNA-binding proteins that involve in diverse biological processes. We have reported that p53 activates ARID3A transcription, and ARID3A overexpression induced G1 arrest. However, a role of ARID3A in the p53 pathway remains u...

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Main Authors:	Lestari, Widya, Arief Ichwan, Solachuddin Jauhari
Format:	Conference or Workshop Item
Language:	English
Published:	2013
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/36693/ http://irep.iium.edu.my/36693/1/36693.pdf

id	iium-36693
recordtype	eprints
spelling	iium-366932015-05-12T03:06:54Z http://irep.iium.edu.my/36693/ Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage Lestari, Widya Arief Ichwan, Solachuddin Jauhari RK Dentistry ARID3A/DRIL1/Bright is a family member of AT rich interaction domain (ARID) DNA-binding proteins that involve in diverse biological processes. We have reported that p53 activates ARID3A transcription, and ARID3A overexpression induced G1 arrest. However, a role of ARID3A in the p53 pathway remains unclear. Here, ‘we show that ARID3A cooperates with p53 to transcriptionally activate p21WAF1, a p53-target gene important for cell-cycle arrest. ARID3A bound to its binding sites in the p21WAF1 promoter in vivo and in vitro, and induced p21WAF1 transcription in U2OS cells expressing wild-type p53 but not Saos-2 cells lacking p53. Co-expression of ARID3A with p53 cooperates to activate p21WAF1 transcription and the stably transfected p21WAF1 promoter. Mutation of the ARID3A binding sites reduced the p21WAF1 promoter activity, and siRNA-based ARID3A knockdown suppressed transcription of p21WAF1 but not proapoptotic NOXA and PUMA in response to DNA damage. Furthermore, p53 knockdown decreased ARID3A transcription, and, conversely, ARID3A overexpression or knockdown resulted in an increase or decrease in p53 stability, respectively. These results indicate cooperative and interdependent roles for ARID3A and p53 in transcriptional activation of p21WAF1 in response to DNA damage. 2013 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/36693/1/36693.pdf Lestari, Widya and Arief Ichwan, Solachuddin Jauhari (2013) Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage. In: IIUM Research, Invention and Innovation Exhibition (IRIIE) 2013, 19-20th February 2013, Cultural Activity Centre (CAC) and KAED Gallery, IIUM. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Lestari, Widya Arief Ichwan, Solachuddin Jauhari Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage
description	ARID3A/DRIL1/Bright is a family member of AT rich interaction domain (ARID) DNA-binding proteins that involve in diverse biological processes. We have reported that p53 activates ARID3A transcription, and ARID3A overexpression induced G1 arrest. However, a role of ARID3A in the p53 pathway remains unclear. Here, ‘we show that ARID3A cooperates with p53 to transcriptionally activate p21WAF1, a p53-target gene important for cell-cycle arrest. ARID3A bound to its binding sites in the p21WAF1 promoter in vivo and in vitro, and induced p21WAF1 transcription in U2OS cells expressing wild-type p53 but not Saos-2 cells lacking p53. Co-expression of ARID3A with p53 cooperates to activate p21WAF1 transcription and the stably transfected p21WAF1 promoter. Mutation of the ARID3A binding sites reduced the p21WAF1 promoter activity, and siRNA-based ARID3A knockdown suppressed transcription of p21WAF1 but not proapoptotic NOXA and PUMA in response to DNA damage. Furthermore, p53 knockdown decreased ARID3A transcription, and, conversely, ARID3A overexpression or knockdown resulted in an increase or decrease in p53 stability, respectively. These results indicate cooperative and interdependent roles for ARID3A and p53 in transcriptional activation of p21WAF1 in response to DNA damage.
format	Conference or Workshop Item
author	Lestari, Widya Arief Ichwan, Solachuddin Jauhari
author_facet	Lestari, Widya Arief Ichwan, Solachuddin Jauhari
author_sort	Lestari, Widya
title	Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage
title_short	Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage
title_full	Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage
title_fullStr	Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage
title_full_unstemmed	Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage
title_sort	cooperation between arid3a and p53 in the transcriptional activation of p21waf1 in response to dna damage
publishDate	2013
url	http://irep.iium.edu.my/36693/ http://irep.iium.edu.my/36693/1/36693.pdf
first_indexed	2023-09-18T20:52:35Z
last_indexed	2023-09-18T20:52:35Z
_version_	1777410092914704384

Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage

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