A lab-on-chip cell-based biosensor for label-free sensing of water toxicants
This paper presents a lab-on-chip biosensor containing an enclosed fluidic cell culturing well seeded with live cells for rapid screening of toxicants in drinking water. The sensor is based on the innovative placement of the working electrode for the electrical cell–substrate impedance sensing (EC...
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| Format: | Article |
| Language: | English |
| Published: |
Royal Society of Chemistry
2014
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| Online Access: | http://irep.iium.edu.my/36648/ http://irep.iium.edu.my/36648/ http://irep.iium.edu.my/36648/ http://irep.iium.edu.my/36648/1/C3LC51085A%281%29.pdf |
| Summary: | This paper presents a lab-on-chip biosensor containing an enclosed fluidic cell culturing well seeded
with live cells for rapid screening of toxicants in drinking water. The sensor is based on the innovative
placement of the working electrode for the electrical cell–substrate impedance sensing (ECIS) technique
as the top electrode of a quartz crystal microbalance (QCM) resonator. Cell damage induced by toxic
water will cause a decrease in impedance, as well as an increase in the resonant frequency. For water
toxicity tests, the biosensor's unique capabilities of performing two complementary measurements
simultaneously (impedance and mass-sensing) will increase the accuracy of detection while decreasing
the false-positive rate. Bovine aortic endothelial cells (BAECs) were used as toxicity sensing cells. The
effects of the toxicants, ammonia, nicotine and aldicarb, on cells were monitored with both the QCM
and the ECIS technique. The lab-on-chip was demonstrated to be sensitive to low concentrations of
toxicants. The responses of BAECs to toxic samples occurred during the initial 5 to 20 minutes
depending on the type of chemical and concentrations. Testing the multiparameter biosensor with
aldicarb also demonstrated the hypothesis that using two different sensors to monitor the same cell
monolayer provides cross validation and increases the accuracy of detection. For low concentrations
of aldicarb, the variations in impedance measurements are insignificant in comparison with the shifts
of resonant frequency monitored using the QCM resonator. A highly linear correlation between signal
shifts and chemical concentrations was demonstrated for each toxicant. |
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