α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells

α-Mangostin is a major compound found in mangosteen fruit hull and it has been reported to have wide range of pharmacological activities. The current study seeks to evaluate the in vitro activities of α-mangostin on adipocyte differentiation, glucose uptake and related gene expressions. The inhibit...

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Main Authors:	Mohamed Amiroudine, Mohamed Zaffar Ali, Bakhtiar, M. Taher, Darnis, Deny Susanti, Arief Ichwan, Solachuddin Jauhari, Kaderi, Mohd Arifin, Zakaria, Zainul Amiruddin
Format:	Conference or Workshop Item
Language:	English English
Published:	2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/36403/ http://irep.iium.edu.my/36403/ http://irep.iium.edu.my/36403/1/-PE_43_5FIP.pdf http://irep.iium.edu.my/36403/2/Binder1.pdf

id	iium-36403
recordtype	eprints
spelling	iium-364032018-06-19T04:26:26Z http://irep.iium.edu.my/36403/ α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Zakaria, Zainul Amiruddin RS Pharmacy and materia medica α-Mangostin is a major compound found in mangosteen fruit hull and it has been reported to have wide range of pharmacological activities. The current study seeks to evaluate the in vitro activities of α-mangostin on adipocyte differentiation, glucose uptake and related gene expressions. The inhibitory effect of the compound on lipid accumulation of 3T3-L1 preadipocytes was determined by Oil Red O staining method. Glucose uptake and free fatty acid release from the medium were determined by using 2-deoxy-D-[3H] glucose and adipolysis kit, respectively. Finally, the evaluation of related gene expression was carried out by the quantitative reverse transcriptase polymerase chain reaction (gRT-PCR). Depending on the dose, cell treated with α-mangostin was found to inhibit the cytoplasmic lipid accumulation as well as adipogenic differentiation of preadipocyte. Analyses of 2-deoxy-D-[3H] glucose uptake activity showed that α-mangostin significantly improved the glucose uptake (p<0.05). In addition, α-mangostin increased the amount of free fatty acid released. qRT-PCR analysis showed that the α-mangostin reduced lipid accumulation by inhibition of PPARγ gene expression. The induction of glucose uptake and free fatty acid release by α-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin. In summary, these evidences proposed that α-mangostin might be beneficial for obesity management. 2014-04-13 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/36403/1/-PE_43_5FIP.pdf application/pdf en http://irep.iium.edu.my/36403/2/Binder1.pdf Mohamed Amiroudine, Mohamed Zaffar Ali and Bakhtiar, M. Taher and Darnis, Deny Susanti and Arief Ichwan, Solachuddin Jauhari and Kaderi, Mohd Arifin and Zakaria, Zainul Amiruddin (2014) α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells. In: 5th FIP Pharmaceutical Sciences World Congress (PSWC 2014), 13th-16th Apr. 2014, Melbourne, Australia. http://www.fip.org/pswc2014/Melbourne/2026/Abstracts/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Zakaria, Zainul Amiruddin α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells
description	α-Mangostin is a major compound found in mangosteen fruit hull and it has been reported to have wide range of pharmacological activities. The current study seeks to evaluate the in vitro activities of α-mangostin on adipocyte differentiation, glucose uptake and related gene expressions. The inhibitory effect of the compound on lipid accumulation of 3T3-L1 preadipocytes was determined by Oil Red O staining method. Glucose uptake and free fatty acid release from the medium were determined by using 2-deoxy-D-[3H] glucose and adipolysis kit, respectively. Finally, the evaluation of related gene expression was carried out by the quantitative reverse transcriptase polymerase chain reaction (gRT-PCR). Depending on the dose, cell treated with α-mangostin was found to inhibit the cytoplasmic lipid accumulation as well as adipogenic differentiation of preadipocyte. Analyses of 2-deoxy-D-[3H] glucose uptake activity showed that α-mangostin significantly improved the glucose uptake (p<0.05). In addition, α-mangostin increased the amount of free fatty acid released. qRT-PCR analysis showed that the α-mangostin reduced lipid accumulation by inhibition of PPARγ gene expression. The induction of glucose uptake and free fatty acid release by α-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin. In summary, these evidences proposed that α-mangostin might be beneficial for obesity management.
format	Conference or Workshop Item
author	Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Zakaria, Zainul Amiruddin
author_facet	Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Zakaria, Zainul Amiruddin
author_sort	Mohamed Amiroudine, Mohamed Zaffar Ali
title	α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells
title_short	α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells
title_full	α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells
title_fullStr	α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells
title_full_unstemmed	α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells
title_sort	α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3t3-l1 cells
publishDate	2014
url	http://irep.iium.edu.my/36403/ http://irep.iium.edu.my/36403/ http://irep.iium.edu.my/36403/1/-PE_43_5FIP.pdf http://irep.iium.edu.my/36403/2/Binder1.pdf
first_indexed	2023-09-18T20:52:09Z
last_indexed	2023-09-18T20:52:09Z
_version_	1777410065935892480

α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells

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