Comparing BRIN-BD11 culture producing insulin using different type of microcarriers

This research was conducted to examine the growth profile, growth kinetics, and insulinsecretory responsiveness of BRIN-BD11 cells grown in optimized medium on different types of microcarriers (MCs). Comparisons were made on modified polystyrene (Hillex� II) and crosslinked polystyrene Plastic...

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Main Authors:	Mel, Maizirwan, Abdul Karim, Mohamed Ismail, Mohd Yusof, Siti Aishah, Hashim, Yumi Zuhanis Has-Yun, Ahmad Nor, Yusilawati
Format:	Article
Language:	English
Published:	Springer 2010
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/1/paper_at_cytotechnolopgy_%28dec_2010%29.pdf

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recordtype	eprints
spelling	iium-36392011-09-22T03:54:04Z http://irep.iium.edu.my/3639/ Comparing BRIN-BD11 culture producing insulin using different type of microcarriers Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yusof, Siti Aishah Hashim, Yumi Zuhanis Has-Yun Ahmad Nor, Yusilawati TP155 Chemical engineering TP248.13 Biotechnology This research was conducted to examine the growth profile, growth kinetics, and insulinsecretory responsiveness of BRIN-BD11 cells grown in optimized medium on different types of microcarriers (MCs). Comparisons were made on modified polystyrene (Hillex� II) and crosslinked polystyrene Plastic Plus (PP) from Solohill Engineering. The cell line producing insulin was cultured in a 25 cm2 T-flask as control while MCs based culture was implemented in a stirred tank bioreactor with 1 L working volume. For each culture type, the viable cell number, glucose, lactate, glutamate, and insulin concentrations were measured and compared. Maximum viable cell number was obtained at 1.47 9 105 cell/mL for PP microcarrier (PPMCs) culture, 1.35 9 105 cell/mL Hillex� II (HIIMCs) culture and 0.95 9 105 cell/mL for T-flask culture, respectively. The highest insulin concentration has been produced in PPMCs culture (5.31 mg/L) compared toHIIMCs culture (2.01 mg/L) and T-flask culture (1.99 mg/L). Therefore overall observation suggested that PPMCs was likely preferred to be used for BRIN-BD11 cell culture as compared with Hillex� II MCs. Springer 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3639/1/paper_at_cytotechnolopgy_%28dec_2010%29.pdf Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Mohd Yusof, Siti Aishah and Hashim, Yumi Zuhanis Has-Yun and Ahmad Nor, Yusilawati (2010) Comparing BRIN-BD11 culture producing insulin using different type of microcarriers. Cytotechnology, 62. pp. 423-430. ISSN 1573-0778 (O), 0920-9069 (P) http://www.springerlink.com/content/h701w8l2x847h811/ DOI: 10.1007/s10616-010-9294-9
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yusof, Siti Aishah Hashim, Yumi Zuhanis Has-Yun Ahmad Nor, Yusilawati Comparing BRIN-BD11 culture producing insulin using different type of microcarriers
description	This research was conducted to examine the growth profile, growth kinetics, and insulinsecretory responsiveness of BRIN-BD11 cells grown in optimized medium on different types of microcarriers (MCs). Comparisons were made on modified polystyrene (Hillex� II) and crosslinked polystyrene Plastic Plus (PP) from Solohill Engineering. The cell line producing insulin was cultured in a 25 cm2 T-flask as control while MCs based culture was implemented in a stirred tank bioreactor with 1 L working volume. For each culture type, the viable cell number, glucose, lactate, glutamate, and insulin concentrations were measured and compared. Maximum viable cell number was obtained at 1.47 9 105 cell/mL for PP microcarrier (PPMCs) culture, 1.35 9 105 cell/mL Hillex� II (HIIMCs) culture and 0.95 9 105 cell/mL for T-flask culture, respectively. The highest insulin concentration has been produced in PPMCs culture (5.31 mg/L) compared toHIIMCs culture (2.01 mg/L) and T-flask culture (1.99 mg/L). Therefore overall observation suggested that PPMCs was likely preferred to be used for BRIN-BD11 cell culture as compared with Hillex� II MCs.
format	Article
author	Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yusof, Siti Aishah Hashim, Yumi Zuhanis Has-Yun Ahmad Nor, Yusilawati
author_facet	Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yusof, Siti Aishah Hashim, Yumi Zuhanis Has-Yun Ahmad Nor, Yusilawati
author_sort	Mel, Maizirwan
title	Comparing BRIN-BD11 culture producing insulin using different type of microcarriers
title_short	Comparing BRIN-BD11 culture producing insulin using different type of microcarriers
title_full	Comparing BRIN-BD11 culture producing insulin using different type of microcarriers
title_fullStr	Comparing BRIN-BD11 culture producing insulin using different type of microcarriers
title_full_unstemmed	Comparing BRIN-BD11 culture producing insulin using different type of microcarriers
title_sort	comparing brin-bd11 culture producing insulin using different type of microcarriers
publisher	Springer
publishDate	2010
url	http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/1/paper_at_cytotechnolopgy_%28dec_2010%29.pdf
first_indexed	2023-09-18T20:11:32Z
last_indexed	2023-09-18T20:11:32Z
_version_	1777407510498508800

Comparing BRIN-BD11 culture producing insulin using different type of microcarriers

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