Cytotoxic agents from luvunga scandens human breast adenocarcinoma cell line (MCF-7)

Breast cancer is among of the frequently diagnosed cancer and causes high mortality rate in most countries in the world. These led to the necessity for the development of potential chemotherapeutic agents against breast cancer cells with novel mechanisms of actions. The plant-derived triterpenoids c...

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Main Authors:	Al Zikri, Putri Nurhidayah, Arief Ichwan, Solachuddin Jauhari, Darnis, Deny Susanti, Bakhtiar, M. Taher
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/36160/ http://irep.iium.edu.my/36160/ http://irep.iium.edu.my/36160/1/Binder1.pdf

id	iium-36160
recordtype	eprints
spelling	iium-361602018-06-20T01:52:47Z http://irep.iium.edu.my/36160/ Cytotoxic agents from luvunga scandens human breast adenocarcinoma cell line (MCF-7) Al Zikri, Putri Nurhidayah Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Bakhtiar, M. Taher RS Pharmacy and materia medica Breast cancer is among of the frequently diagnosed cancer and causes high mortality rate in most countries in the world. These led to the necessity for the development of potential chemotherapeutic agents against breast cancer cells with novel mechanisms of actions. The plant-derived triterpenoids commonly used due to its ability to exhibit cytotoxicity against a variety of tumor cells. Luvunga scandens (Rutaceae) is one of the plants that contain triterpenoids. L. scandens was extracted and assayed on human breast adenocarcinoma cell line (MCF-7). The stems of L. scandens were extracted with n-hexane, dichloromethane (DCM) and ethanol (EtOH). DCM extracts were then further fractionated by vacuum liquid chromatography (VLC) into five extracts; namely n-hexane, n-hexane:DCM, DCM, DCM:MeOH and MeOH. Those five extracts were assayed in vitro cytototoxic activity against MCF-7. Among them, DCM:MeOH fraction was found to be the most effective and was further subjected to isolation procedure and the isolated compound was assayed in vitro cytotoxic activity against MCF-7. Bioactivity-guided fractionation of the fraction through successive column chromatography procedures led to the isolation of a triterpene-type of compounds. The structure of the compound was elucidated to be lanosta-8, 24-dien-21-oicacid, 3-oxo, (13a, 14b, 17a, 20S) with molecular formula of C3OH46O3. This compound was found to be responsible for the cytotoxicity effects with the IC50 value of 24.8 μM. Whereas IC50 of DCM extract and DCM:MeOH fraction extract is 75 and 94 μg/ml respectively. Therefore, it revealed that L. scandens is potential sources of cytotoxic agents. 2014-03 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/36160/1/Binder1.pdf Al Zikri, Putri Nurhidayah and Arief Ichwan, Solachuddin Jauhari and Darnis, Deny Susanti and Bakhtiar, M. Taher (2014) Cytotoxic agents from luvunga scandens human breast adenocarcinoma cell line (MCF-7). In: International Conference on Natural Products 2014, 18th-19th March 2014, Palm Garden Hotel, IOI Resorts, Putrajaya, Malaysia. http://www.ukm.my/icnp2014/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Al Zikri, Putri Nurhidayah Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Bakhtiar, M. Taher Cytotoxic agents from luvunga scandens human breast adenocarcinoma cell line (MCF-7)
description	Breast cancer is among of the frequently diagnosed cancer and causes high mortality rate in most countries in the world. These led to the necessity for the development of potential chemotherapeutic agents against breast cancer cells with novel mechanisms of actions. The plant-derived triterpenoids commonly used due to its ability to exhibit cytotoxicity against a variety of tumor cells. Luvunga scandens (Rutaceae) is one of the plants that contain triterpenoids. L. scandens was extracted and assayed on human breast adenocarcinoma cell line (MCF-7). The stems of L. scandens were extracted with n-hexane, dichloromethane (DCM) and ethanol (EtOH). DCM extracts were then further fractionated by vacuum liquid chromatography (VLC) into five extracts; namely n-hexane, n-hexane:DCM, DCM, DCM:MeOH and MeOH. Those five extracts were assayed in vitro cytototoxic activity against MCF-7. Among them, DCM:MeOH fraction was found to be the most effective and was further subjected to isolation procedure and the isolated compound was assayed in vitro cytotoxic activity against MCF-7. Bioactivity-guided fractionation of the fraction through successive column chromatography procedures led to the isolation of a triterpene-type of compounds. The structure of the compound was elucidated to be lanosta-8, 24-dien-21-oicacid, 3-oxo, (13a, 14b, 17a, 20S) with molecular formula of C3OH46O3. This compound was found to be responsible for the cytotoxicity effects with the IC50 value of 24.8 μM. Whereas IC50 of DCM extract and DCM:MeOH fraction extract is 75 and 94 μg/ml respectively. Therefore, it revealed that L. scandens is potential sources of cytotoxic agents.
format	Conference or Workshop Item
author	Al Zikri, Putri Nurhidayah Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Bakhtiar, M. Taher
author_facet	Al Zikri, Putri Nurhidayah Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Bakhtiar, M. Taher
author_sort	Al Zikri, Putri Nurhidayah
title	Cytotoxic agents from luvunga scandens human breast adenocarcinoma cell line (MCF-7)
title_short	Cytotoxic agents from luvunga scandens human breast adenocarcinoma cell line (MCF-7)
title_full	Cytotoxic agents from luvunga scandens human breast adenocarcinoma cell line (MCF-7)
title_fullStr	Cytotoxic agents from luvunga scandens human breast adenocarcinoma cell line (MCF-7)
title_full_unstemmed	Cytotoxic agents from luvunga scandens human breast adenocarcinoma cell line (MCF-7)
title_sort	cytotoxic agents from luvunga scandens human breast adenocarcinoma cell line (mcf-7)
publishDate	2014
url	http://irep.iium.edu.my/36160/ http://irep.iium.edu.my/36160/ http://irep.iium.edu.my/36160/1/Binder1.pdf
first_indexed	2023-09-18T20:51:46Z
last_indexed	2023-09-18T20:51:46Z
_version_	1777410041819693056

Cytotoxic agents from luvunga scandens human breast adenocarcinoma cell line (MCF-7)

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