Optimization of process conditions for extraction of xanthine oxidase inhibitor form potential Malaysian medicinal plant

Xanthine oxidase (XO) is an enzyme involved in the formation of uric acid in the body by catalyzing the oxidation of hypoxanthine to xanthine, and then, xanthine to uric acid. Excess serum accumulated with uric acid can lead to a type of arthritis known as gout. Hence, introducing natural xanthine o...

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Main Authors:	Jamal, Parveen, Amid, Azura
Format:	Monograph
Language:	English
Published:	s.n 2012
Subjects:	TP155 Chemical engineering
Online Access:	http://irep.iium.edu.my/36095/ http://irep.iium.edu.my/36095/ http://irep.iium.edu.my/36095/1/EDW_A11-009-0800.pdf

id	iium-36095
recordtype	eprints
spelling	iium-360952014-12-24T03:25:43Z http://irep.iium.edu.my/36095/ Optimization of process conditions for extraction of xanthine oxidase inhibitor form potential Malaysian medicinal plant Jamal, Parveen Amid, Azura TP155 Chemical engineering Xanthine oxidase (XO) is an enzyme involved in the formation of uric acid in the body by catalyzing the oxidation of hypoxanthine to xanthine, and then, xanthine to uric acid. Excess serum accumulated with uric acid can lead to a type of arthritis known as gout. Hence, introducing natural xanthine oxidase inhibitor (XOI) as new source of gout medication is highly granted. The leaves of five local medicinal plants were examined for the inhibition of XO under four process conditions, namely, extraction temperature, extraction time, agitation speed, and ratio of the sample to the solvent. Distilled water and 70% methanol were employed in the extraction process. The degree of inhibition was determined by measuring the absorbance spectrophotometrically at 290 nm associated with uric acid formation. Aqueous-methanolic extract of Morinda elliptica leaves was found to have the highest XO inhibitory activity at 100 μg/ml with 86.94 ± 0.34%, thus selected for optimization of process conditions for extraction by using Central Composite Design (CCD) from Design Expert v.6.0.8. The second highest was aqueous-methanolic extract of Mentha spicata leaves with 86.49 ± 0.84% followed by aqueous-methanolic extract of Carica papaya leaves with 79.28 ± 0.29%. Development of process conditions for extraction of XOI from leaves of Morinda elliptica was performed and the analysis of variance demonstrated that the model F-value of 18.31 showed the significance of the model with R2 of 97.71%. The analysis revealed that the XO inhibitory activity was enhanced to 88.93%, reached at 32 ºC, 30 h, 125 rpm and 1 g/15 ml of solvent. The results were encouraging to formulate food or nutraceutical products incorporating natural XOI, an alternative to irresponsive synthetic XOI. s.n 2012-01-27 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/36095/1/EDW_A11-009-0800.pdf Jamal, Parveen and Amid, Azura (2012) Optimization of process conditions for extraction of xanthine oxidase inhibitor form potential Malaysian medicinal plant. Research Report. s.n, Kuala Lumpur. (Unpublished) EDW A11-009-0800
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering
spellingShingle	TP155 Chemical engineering Jamal, Parveen Amid, Azura Optimization of process conditions for extraction of xanthine oxidase inhibitor form potential Malaysian medicinal plant
description	Xanthine oxidase (XO) is an enzyme involved in the formation of uric acid in the body by catalyzing the oxidation of hypoxanthine to xanthine, and then, xanthine to uric acid. Excess serum accumulated with uric acid can lead to a type of arthritis known as gout. Hence, introducing natural xanthine oxidase inhibitor (XOI) as new source of gout medication is highly granted. The leaves of five local medicinal plants were examined for the inhibition of XO under four process conditions, namely, extraction temperature, extraction time, agitation speed, and ratio of the sample to the solvent. Distilled water and 70% methanol were employed in the extraction process. The degree of inhibition was determined by measuring the absorbance spectrophotometrically at 290 nm associated with uric acid formation. Aqueous-methanolic extract of Morinda elliptica leaves was found to have the highest XO inhibitory activity at 100 μg/ml with 86.94 ± 0.34%, thus selected for optimization of process conditions for extraction by using Central Composite Design (CCD) from Design Expert v.6.0.8. The second highest was aqueous-methanolic extract of Mentha spicata leaves with 86.49 ± 0.84% followed by aqueous-methanolic extract of Carica papaya leaves with 79.28 ± 0.29%. Development of process conditions for extraction of XOI from leaves of Morinda elliptica was performed and the analysis of variance demonstrated that the model F-value of 18.31 showed the significance of the model with R2 of 97.71%. The analysis revealed that the XO inhibitory activity was enhanced to 88.93%, reached at 32 ºC, 30 h, 125 rpm and 1 g/15 ml of solvent. The results were encouraging to formulate food or nutraceutical products incorporating natural XOI, an alternative to irresponsive synthetic XOI.
format	Monograph
author	Jamal, Parveen Amid, Azura
author_facet	Jamal, Parveen Amid, Azura
author_sort	Jamal, Parveen
title	Optimization of process conditions for extraction of xanthine oxidase inhibitor form potential Malaysian medicinal plant
title_short	Optimization of process conditions for extraction of xanthine oxidase inhibitor form potential Malaysian medicinal plant
title_full	Optimization of process conditions for extraction of xanthine oxidase inhibitor form potential Malaysian medicinal plant
title_fullStr	Optimization of process conditions for extraction of xanthine oxidase inhibitor form potential Malaysian medicinal plant
title_full_unstemmed	Optimization of process conditions for extraction of xanthine oxidase inhibitor form potential Malaysian medicinal plant
title_sort	optimization of process conditions for extraction of xanthine oxidase inhibitor form potential malaysian medicinal plant
publisher	s.n
publishDate	2012
url	http://irep.iium.edu.my/36095/ http://irep.iium.edu.my/36095/ http://irep.iium.edu.my/36095/1/EDW_A11-009-0800.pdf
first_indexed	2023-09-18T20:51:40Z
last_indexed	2023-09-18T20:51:40Z
_version_	1777410035756826624

Optimization of process conditions for extraction of xanthine oxidase inhibitor form potential Malaysian medicinal plant

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