CHO-K1 cells expressed IGF-1 Gene: a promising target towards optimal media at effective cost

Insulin-like growth factor-1 (IGF-1) has been shown to promote cell proliferation and inhibit apoptosis of cells. These are two characteristics in mammalian cell culture which may lead to high density cell culture producing optimal desired yield of bioproducts. IGF-1 has also been recognized in tre...

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Main Authors:	Packeer Mohamed, Vasila, Hasyim, Yumi Zuhanis Has-Yun, Amid, Azura, Mel, Maizirwan, Kamarulzaman, Abdul Wahab, Abdul Wahab, Muhammad Adil, Mohamed Saberi, Salfarina Ezrina
Format:	Conference or Workshop Item
Language:	English English
Published:	2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/3605/ http://irep.iium.edu.my/3605/1/Paper_CHO_Vasila.pdf http://irep.iium.edu.my/3605/3/CHO-K1_Cells_Expressed.pdf

id	iium-3605
recordtype	eprints
spelling	iium-36052012-01-25T10:25:52Z http://irep.iium.edu.my/3605/ CHO-K1 cells expressed IGF-1 Gene: a promising target towards optimal media at effective cost Packeer Mohamed, Vasila Hasyim, Yumi Zuhanis Has-Yun Amid, Azura Mel, Maizirwan Kamarulzaman, Abdul Wahab Abdul Wahab, Muhammad Adil Mohamed Saberi, Salfarina Ezrina TP248.13 Biotechnology Insulin-like growth factor-1 (IGF-1) has been shown to promote cell proliferation and inhibit apoptosis of cells. These are two characteristics in mammalian cell culture which may lead to high density cell culture producing optimal desired yield of bioproducts. IGF-1 has also been recognized in treatment of growth failure and cancer. This study is set to investigate IGF-1 gene expression in Chinese Hamster Ovary (CHO-K1) cells and its relationship with cellular behavior at different time points of cell growth. CHO-K1 cells were first cultured in T-flask with three independent experiments. An 8-hourly sampling for responses (glucose and lactate), (total protein and biomass) were taken. For IGF-1 gene detection, PCR-based method for selected time points was performed. Results from the study showed that CHO-K1 cells expressed IGF-1 gene. This suggests that IGF-1 plays a role in cell roliferation. The study confirms the IGF-1 expression in CHO-K1 cells. It also provides the baseline data for CHO-K1 cell growth which is important for future studies using CHO-K1 cells especially it is a promising target towards optimal media at effective cost. 2011-05-17 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/3605/1/Paper_CHO_Vasila.pdf application/pdf en http://irep.iium.edu.my/3605/3/CHO-K1_Cells_Expressed.pdf Packeer Mohamed, Vasila and Hasyim, Yumi Zuhanis Has-Yun and Amid, Azura and Mel, Maizirwan and Kamarulzaman, Abdul Wahab and Abdul Wahab, Muhammad Adil and Mohamed Saberi, Salfarina Ezrina (2011) CHO-K1 cells expressed IGF-1 Gene: a promising target towards optimal media at effective cost. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May, 2011, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Packeer Mohamed, Vasila Hasyim, Yumi Zuhanis Has-Yun Amid, Azura Mel, Maizirwan Kamarulzaman, Abdul Wahab Abdul Wahab, Muhammad Adil Mohamed Saberi, Salfarina Ezrina CHO-K1 cells expressed IGF-1 Gene: a promising target towards optimal media at effective cost
description	Insulin-like growth factor-1 (IGF-1) has been shown to promote cell proliferation and inhibit apoptosis of cells. These are two characteristics in mammalian cell culture which may lead to high density cell culture producing optimal desired yield of bioproducts. IGF-1 has also been recognized in treatment of growth failure and cancer. This study is set to investigate IGF-1 gene expression in Chinese Hamster Ovary (CHO-K1) cells and its relationship with cellular behavior at different time points of cell growth. CHO-K1 cells were first cultured in T-flask with three independent experiments. An 8-hourly sampling for responses (glucose and lactate), (total protein and biomass) were taken. For IGF-1 gene detection, PCR-based method for selected time points was performed. Results from the study showed that CHO-K1 cells expressed IGF-1 gene. This suggests that IGF-1 plays a role in cell roliferation. The study confirms the IGF-1 expression in CHO-K1 cells. It also provides the baseline data for CHO-K1 cell growth which is important for future studies using CHO-K1 cells especially it is a promising target towards optimal media at effective cost.
format	Conference or Workshop Item
author	Packeer Mohamed, Vasila Hasyim, Yumi Zuhanis Has-Yun Amid, Azura Mel, Maizirwan Kamarulzaman, Abdul Wahab Abdul Wahab, Muhammad Adil Mohamed Saberi, Salfarina Ezrina
author_facet	Packeer Mohamed, Vasila Hasyim, Yumi Zuhanis Has-Yun Amid, Azura Mel, Maizirwan Kamarulzaman, Abdul Wahab Abdul Wahab, Muhammad Adil Mohamed Saberi, Salfarina Ezrina
author_sort	Packeer Mohamed, Vasila
title	CHO-K1 cells expressed IGF-1 Gene: a promising target towards optimal media at effective cost
title_short	CHO-K1 cells expressed IGF-1 Gene: a promising target towards optimal media at effective cost
title_full	CHO-K1 cells expressed IGF-1 Gene: a promising target towards optimal media at effective cost
title_fullStr	CHO-K1 cells expressed IGF-1 Gene: a promising target towards optimal media at effective cost
title_full_unstemmed	CHO-K1 cells expressed IGF-1 Gene: a promising target towards optimal media at effective cost
title_sort	cho-k1 cells expressed igf-1 gene: a promising target towards optimal media at effective cost
publishDate	2011
url	http://irep.iium.edu.my/3605/ http://irep.iium.edu.my/3605/1/Paper_CHO_Vasila.pdf http://irep.iium.edu.my/3605/3/CHO-K1_Cells_Expressed.pdf
first_indexed	2023-09-18T20:11:30Z
last_indexed	2023-09-18T20:11:30Z
_version_	1777407507903283200

CHO-K1 cells expressed IGF-1 Gene: a promising target towards optimal media at effective cost

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