Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus

Lactic acid bacteria (LAB) were isolated from the bile, gizzard, intestine and caecum of the Malaysian domestic non-broiler chicken. Of the 56 isolates grown on MRS agar plates, 7 – namely, B3L3, B4L4, G5L5, B5L6, B10L7, I1L8, and C4L10 – showed inhibition against the methicillin resistant Staphy...

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Main Authors:	Alhaji Yusuf, Moshood, Tengku Abdul Hamid, Tengku Haziyamin
Format:	Article
Language:	English
Published:	Versita 2013
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/35913/ http://irep.iium.edu.my/35913/ http://irep.iium.edu.my/35913/1/J-Agrobio_CzechRep.pdf

id	iium-35913
recordtype	eprints
spelling	iium-359132014-11-27T08:42:12Z http://irep.iium.edu.my/35913/ Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus Alhaji Yusuf, Moshood Tengku Abdul Hamid, Tengku Haziyamin Q Science (General) Lactic acid bacteria (LAB) were isolated from the bile, gizzard, intestine and caecum of the Malaysian domestic non-broiler chicken. Of the 56 isolates grown on MRS agar plates, 7 – namely, B3L3, B4L4, G5L5, B5L6, B10L7, I1L8, and C4L10 – showed inhibition against the methicillin resistant Staphylococcus aureus (MRSA). These isolates were all Gram positive cocci, non-spore forming, and catalase negative. The purified fraction from the three phase partitioning (TPP) method, employing t-butanol and ammonium sulfate, produced inhibitory zones of 8 to 18 mm in diameter on the indicator organism, Staphylococcus aureus (MRSA). The TPP purified fraction contained heat stable proteins of about 10 kDa in size and was inactivated by trypsin. Based on the partial rDNA sequences (>89% similarity), isolates B4L4 and G5L5 belonged to Enterococcus hirae; isolate B3L3 belonged to Enterococcus faecium; isolates B5L6, B10L7, I1L8 to Enterococcus faecalis; and isolate C4L10 to Enterococcus mundtii. These probiotic strains, exclusively isolated from the Malaysian non-broiler chicken, produced an antimicrobial protein or bacteriocin which was active against a pathogenic strain of methicillin resistant Staphylococcus aureus (MRSA). Versita 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/35913/1/J-Agrobio_CzechRep.pdf Alhaji Yusuf, Moshood and Tengku Abdul Hamid, Tengku Haziyamin (2013) Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus. Journal of Agrobiology , 30 (1). pp. 33-42. ISSN ISSN 1803-4403 http://versita.com/science/agriculture/joa
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Alhaji Yusuf, Moshood Tengku Abdul Hamid, Tengku Haziyamin Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus
description	Lactic acid bacteria (LAB) were isolated from the bile, gizzard, intestine and caecum of the Malaysian domestic non-broiler chicken. Of the 56 isolates grown on MRS agar plates, 7 – namely, B3L3, B4L4, G5L5, B5L6, B10L7, I1L8, and C4L10 – showed inhibition against the methicillin resistant Staphylococcus aureus (MRSA). These isolates were all Gram positive cocci, non-spore forming, and catalase negative. The purified fraction from the three phase partitioning (TPP) method, employing t-butanol and ammonium sulfate, produced inhibitory zones of 8 to 18 mm in diameter on the indicator organism, Staphylococcus aureus (MRSA). The TPP purified fraction contained heat stable proteins of about 10 kDa in size and was inactivated by trypsin. Based on the partial rDNA sequences (>89% similarity), isolates B4L4 and G5L5 belonged to Enterococcus hirae; isolate B3L3 belonged to Enterococcus faecium; isolates B5L6, B10L7, I1L8 to Enterococcus faecalis; and isolate C4L10 to Enterococcus mundtii. These probiotic strains, exclusively isolated from the Malaysian non-broiler chicken, produced an antimicrobial protein or bacteriocin which was active against a pathogenic strain of methicillin resistant Staphylococcus aureus (MRSA).
format	Article
author	Alhaji Yusuf, Moshood Tengku Abdul Hamid, Tengku Haziyamin
author_facet	Alhaji Yusuf, Moshood Tengku Abdul Hamid, Tengku Haziyamin
author_sort	Alhaji Yusuf, Moshood
title	Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus
title_short	Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus
title_full	Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus
title_fullStr	Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus
title_full_unstemmed	Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus
title_sort	isolation of coagulase negative enterococcous sp. strains from non-broiler chicken producing bacteriocin active against staphylococcus aureus
publisher	Versita
publishDate	2013
url	http://irep.iium.edu.my/35913/ http://irep.iium.edu.my/35913/ http://irep.iium.edu.my/35913/1/J-Agrobio_CzechRep.pdf
first_indexed	2023-09-18T20:51:26Z
last_indexed	2023-09-18T20:51:26Z
_version_	1777410020275650560

Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus

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