Protective effect of black seed nigella sativa (l.) Against cyclophosphamide-induced toxicity on reproductive and acrosomal function in mice

To determine the effect of ethanol extract of Nigella sativa on cyclophosphamide reproductive toxicity and acrosome function of sperm. Male Balb/c mice, aged between 5 to 7 weeks were divided into four groups, each of six animals. Cyclophosphamide was administered via intraperitoneal injection at 20...

Full description

Bibliographic Details
Main Authors:	Abdul Rahman, Suzanah, Kamarzaman, Saheera, Shaik , Sadak Basha, Shaik Dawood, Noor Faridah
Format:	Article
Language:	English
Published:	IDOSI Publications 2013
Subjects:	QP Physiology R Medicine (General) RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/35721/ http://irep.iium.edu.my/35721/ http://irep.iium.edu.my/35721/1/MEJSR-Suzanah.pdf

id	iium-35721
recordtype	eprints
spelling	iium-357212016-02-02T04:48:22Z http://irep.iium.edu.my/35721/ Protective effect of black seed nigella sativa (l.) Against cyclophosphamide-induced toxicity on reproductive and acrosomal function in mice Abdul Rahman, Suzanah Kamarzaman, Saheera Shaik , Sadak Basha Shaik Dawood, Noor Faridah QP Physiology R Medicine (General) RM300 Drugs and their action To determine the effect of ethanol extract of Nigella sativa on cyclophosphamide reproductive toxicity and acrosome function of sperm. Male Balb/c mice, aged between 5 to 7 weeks were divided into four groups, each of six animals. Cyclophosphamide was administered via intraperitoneal injection at 200 mg/kg body weight and ethanol extraction of Nigella sativa was similarly injected at a dose of 10 mg/kg and continued on alternate days for the duration of 32 days. All mice were sacrificed on day 33 and testes and epididymides were excised. Group I served as control while group II was treated with cyclophosphamide and group III with 10 mg/kg of Nigella sativa and group IV with a combination of cyclophosphamide and Nigella sativa at the same concentrations. Effects of cyclophosphamide include reduction of weights of testes, epididymides and sperm count. A higher value of sperm count was observed in the cyclophosphamide plus Nigella sativa group when compared to the cyclophosphamide alone group, perhaps indicating the medicinal plant antioxidant properties in the germ cell line. Treatment with cyclophosphamide led to a high percentage of acrosome reacted sperm possibly to spontaneous acrosomal reaction caused by free radicals. Although the combination treatment seemed to show similar histology to the effects of cyclophosphamide alone, a protective outcome was indicated with the higher number of sperm count with the addition of Nigella sativa. Paradoxical effects of Nigella sativa were observed on the cells in the testis and acrosome reaction including in the protection against oxidative agents and alkylating effects of cyclophosphamide. Further investigation is recommended to determine outcomes in the presence of oocytes, at the time when sperm capacitation and acrosome reaction usually occur. IDOSI Publications 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/35721/1/MEJSR-Suzanah.pdf Abdul Rahman, Suzanah and Kamarzaman, Saheera and Shaik , Sadak Basha and Shaik Dawood, Noor Faridah (2013) Protective effect of black seed nigella sativa (l.) Against cyclophosphamide-induced toxicity on reproductive and acrosomal function in mice. Middle-East Journal of Scientific Research, 17 (7). pp. 955-964. ISSN 1990-9233 http://www.idosi.org/mejsr/mejsr.htm
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QP Physiology R Medicine (General) RM300 Drugs and their action
spellingShingle	QP Physiology R Medicine (General) RM300 Drugs and their action Abdul Rahman, Suzanah Kamarzaman, Saheera Shaik , Sadak Basha Shaik Dawood, Noor Faridah Protective effect of black seed nigella sativa (l.) Against cyclophosphamide-induced toxicity on reproductive and acrosomal function in mice
description	To determine the effect of ethanol extract of Nigella sativa on cyclophosphamide reproductive toxicity and acrosome function of sperm. Male Balb/c mice, aged between 5 to 7 weeks were divided into four groups, each of six animals. Cyclophosphamide was administered via intraperitoneal injection at 200 mg/kg body weight and ethanol extraction of Nigella sativa was similarly injected at a dose of 10 mg/kg and continued on alternate days for the duration of 32 days. All mice were sacrificed on day 33 and testes and epididymides were excised. Group I served as control while group II was treated with cyclophosphamide and group III with 10 mg/kg of Nigella sativa and group IV with a combination of cyclophosphamide and Nigella sativa at the same concentrations. Effects of cyclophosphamide include reduction of weights of testes, epididymides and sperm count. A higher value of sperm count was observed in the cyclophosphamide plus Nigella sativa group when compared to the cyclophosphamide alone group, perhaps indicating the medicinal plant antioxidant properties in the germ cell line. Treatment with cyclophosphamide led to a high percentage of acrosome reacted sperm possibly to spontaneous acrosomal reaction caused by free radicals. Although the combination treatment seemed to show similar histology to the effects of cyclophosphamide alone, a protective outcome was indicated with the higher number of sperm count with the addition of Nigella sativa. Paradoxical effects of Nigella sativa were observed on the cells in the testis and acrosome reaction including in the protection against oxidative agents and alkylating effects of cyclophosphamide. Further investigation is recommended to determine outcomes in the presence of oocytes, at the time when sperm capacitation and acrosome reaction usually occur.
format	Article
author	Abdul Rahman, Suzanah Kamarzaman, Saheera Shaik , Sadak Basha Shaik Dawood, Noor Faridah
author_facet	Abdul Rahman, Suzanah Kamarzaman, Saheera Shaik , Sadak Basha Shaik Dawood, Noor Faridah
author_sort	Abdul Rahman, Suzanah
title	Protective effect of black seed nigella sativa (l.) Against cyclophosphamide-induced toxicity on reproductive and acrosomal function in mice
title_short	Protective effect of black seed nigella sativa (l.) Against cyclophosphamide-induced toxicity on reproductive and acrosomal function in mice
title_full	Protective effect of black seed nigella sativa (l.) Against cyclophosphamide-induced toxicity on reproductive and acrosomal function in mice
title_fullStr	Protective effect of black seed nigella sativa (l.) Against cyclophosphamide-induced toxicity on reproductive and acrosomal function in mice
title_full_unstemmed	Protective effect of black seed nigella sativa (l.) Against cyclophosphamide-induced toxicity on reproductive and acrosomal function in mice
title_sort	protective effect of black seed nigella sativa (l.) against cyclophosphamide-induced toxicity on reproductive and acrosomal function in mice
publisher	IDOSI Publications
publishDate	2013
url	http://irep.iium.edu.my/35721/ http://irep.iium.edu.my/35721/ http://irep.iium.edu.my/35721/1/MEJSR-Suzanah.pdf
first_indexed	2023-09-18T20:51:11Z
last_indexed	2023-09-18T20:51:11Z
_version_	1777410004799717376

Protective effect of black seed nigella sativa (l.) Against cyclophosphamide-induced toxicity on reproductive and acrosomal function in mice

Similar Items