Pilot scale production of lipase using palm oil mill effluent as basal medium

Pilot scale production of lipase using palm oil mill effluent as basal medium

Lipase noted as one of the most important enzymes which has widely industrial uses. Production of low cost enzyme using potential raw material will significantly enhance and attractive to the enzyme market. A local residue of palm oil mill effluent (POME) abundantly found and can be used as a...

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Main Authors: Alam, Md. Zahangir, Ratna, Devi, Salleh, Md. Noor
Format: Conference or Workshop Item
Language:English
Published: 2013
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/35000/
http://irep.iium.edu.my/35000/1/ICBioE-14-Devi.PDF
id iium-35000
recordtype eprints
spelling iium-350002014-01-28T01:41:38Z http://irep.iium.edu.my/35000/ Pilot scale production of lipase using palm oil mill effluent as basal medium Alam, Md. Zahangir Ratna, Devi Salleh, Md. Noor TP248.13 Biotechnology Lipase noted as one of the most important enzymes which has widely industrial uses. Production of low cost enzyme using potential raw material will significantly enhance and attractive to the enzyme market. A local residue of palm oil mill effluent (POME) abundantly found and can be used as a basal medium for lipase production. The main objective of this study is to develop a scale up strategy of lipase production (300L of stirred tank bioreactor) for industrial uses. The lipase production was carried out by liquid state bioconversion of POME whereas Candida cylindracea as the microorganism. The fermentation media composition was based on the optimization from the previous study with 1%TSS based POME, 0.5% (W/V) peptone, 0.7% (V/V) Tween-80 and 2.2% inoculums. The fermentation was carried out for 48 hours at 30°C and pH 6. The results showed that maximum lipase production 5.72U/ml and 21.34 U/ml was determined with the scale up strategy of tip speed and P/V constant respectively. 2013 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/35000/1/ICBioE-14-Devi.PDF Alam, Md. Zahangir and Ratna, Devi and Salleh, Md. Noor (2013) Pilot scale production of lipase using palm oil mill effluent as basal medium. In: International Conference on Biotechnology Engineering (ICBioE 2013), 2 – 4 July 2013, Kuala Lumpur, Malaysia.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Alam, Md. Zahangir
Ratna, Devi
Salleh, Md. Noor
Pilot scale production of lipase using palm oil mill effluent as basal medium
description Lipase noted as one of the most important enzymes which has widely industrial uses. Production of low cost enzyme using potential raw material will significantly enhance and attractive to the enzyme market. A local residue of palm oil mill effluent (POME) abundantly found and can be used as a basal medium for lipase production. The main objective of this study is to develop a scale up strategy of lipase production (300L of stirred tank bioreactor) for industrial uses. The lipase production was carried out by liquid state bioconversion of POME whereas Candida cylindracea as the microorganism. The fermentation media composition was based on the optimization from the previous study with 1%TSS based POME, 0.5% (W/V) peptone, 0.7% (V/V) Tween-80 and 2.2% inoculums. The fermentation was carried out for 48 hours at 30°C and pH 6. The results showed that maximum lipase production 5.72U/ml and 21.34 U/ml was determined with the scale up strategy of tip speed and P/V constant respectively.
format Conference or Workshop Item
author Alam, Md. Zahangir
Ratna, Devi
Salleh, Md. Noor
author_facet Alam, Md. Zahangir
Ratna, Devi
Salleh, Md. Noor
author_sort Alam, Md. Zahangir
title Pilot scale production of lipase using palm oil mill effluent as basal medium
title_short Pilot scale production of lipase using palm oil mill effluent as basal medium
title_full Pilot scale production of lipase using palm oil mill effluent as basal medium
title_fullStr Pilot scale production of lipase using palm oil mill effluent as basal medium
title_full_unstemmed Pilot scale production of lipase using palm oil mill effluent as basal medium
title_sort pilot scale production of lipase using palm oil mill effluent as basal medium
publishDate 2013
url http://irep.iium.edu.my/35000/
http://irep.iium.edu.my/35000/1/ICBioE-14-Devi.PDF
first_indexed 2023-09-18T20:50:18Z
last_indexed 2023-09-18T20:50:18Z
_version_ 1777409949719068672

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