In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load

Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV pati...

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Bibliographic Details
Main Authors: Hamzah, Hairul Aini, Mustafa Mahmoud, Mohammed Imad Al-Deen, Abdullah, Nasuruddin
Format: Conference or Workshop Item
Language:English
Published: 2013
Subjects:
Online Access:http://irep.iium.edu.my/34879/
http://irep.iium.edu.my/34879/1/IRIIE-2013-Abstact-Category.pdf
Description
Summary:Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV patients and 53 from patients with negative anti-HCV. Viral RNA was extrcated and subjected for RT-PCR or semi-nested RT-PCR. Results showed high sensitivity of the assay which can detect up to 2.15 IU/ml of viral load. The specificity of the assay was also evaluated. Thus, the extablished protocol was reliable for the detection of HCV-RNA from serum.