In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load
Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV pati...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/34879/ http://irep.iium.edu.my/34879/1/IRIIE-2013-Abstact-Category.pdf |
| Summary: | Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV patients and 53 from patients with negative anti-HCV. Viral RNA was extrcated and subjected for RT-PCR or semi-nested RT-PCR. Results showed high sensitivity of the assay which can detect up to 2.15 IU/ml of viral load. The specificity of the assay was also evaluated. Thus, the extablished protocol was reliable for the detection of HCV-RNA from serum. |
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