Production of α-amylase by immobilized bacillus subtilis in polymeric polyHIPE matrix

The microcellular polymer known as polyHIPE polymer (PHP), with modified physico-chemical characteristics, was developed as a cell matrix for the immobilization of the starch-degrading bacterium, Bacillus subtilis. Suspension of B. subtilis spores was inoculated into a synthesized PHP matrix which i...

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Main Authors:	Jimat, Dzun Noraini, Harwood, Colin, Akay, Galip
Format:	Book Chapter
Language:	English English
Published:	Springer Science+Business Media 2013
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/34785/ http://irep.iium.edu.my/34785/ http://irep.iium.edu.my/34785/ http://irep.iium.edu.my/34785/1/Jimat.pdf http://irep.iium.edu.my/34785/4/bfm%253A978-1-4614-6208-8%252F1.pdf

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spelling	iium-347852015-03-19T06:48:45Z http://irep.iium.edu.my/34785/ Production of α-amylase by immobilized bacillus subtilis in polymeric polyHIPE matrix Jimat, Dzun Noraini Harwood, Colin Akay, Galip TP248.13 Biotechnology The microcellular polymer known as polyHIPE polymer (PHP), with modified physico-chemical characteristics, was developed as a cell matrix for the immobilization of the starch-degrading bacterium, Bacillus subtilis. Suspension of B. subtilis spores was inoculated into a synthesized PHP matrix which is chemically modified, and this PHP matrix was named sulphonated PHPs (SPHPs). These inoculated spores were then activated by supplying continuously well-aerated culture medium (LB medium) and placed in a 37 °C constant temperature room for 24-h incubation. The growth and enzyme productivity data were evaluated and compared. Three different pore and interconnect sizes of SPHPs were evaluated: 42.0 ± 0.61, 36.0 ± 0.50 and 30.0 ± 0.64 μm. The collected samples obtained from the 24-h cultivation were used to determine α-amylase productivity and the loss of cells from the matrices. The morphology, viability and proliferation of the immobilized cells on PHP matrices were observed by scanning electron microscopy (SEM). The SPHP with a pore size of 36.0 μm performed better with respect to the production of α-amylase and the penetration of cells through the whole matrix compared to other SPHPs. The data showed that the total productivity of α-amylase enzyme produced by immobilized cells (on the basis of SPHP volume) was 7.6-fold higher than the batch cell culture. However, if productivity was determined on the basis of used total volume of nutrient medium, that of the immobilized cells was relatively low compared to batch cell culture. Springer Science+Business Media 2013 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/34785/1/Jimat.pdf application/pdf en http://irep.iium.edu.my/34785/4/bfm%253A978-1-4614-6208-8%252F1.pdf Jimat, Dzun Noraini and Harwood, Colin and Akay, Galip (2013) Production of α-amylase by immobilized bacillus subtilis in polymeric polyHIPE matrix. In: Developments in sustainable chemical and bioprocess technology. Springer Science+Business Media, New York, pp. 159-171. ISBN 9781461462088 (O), 9781461462071 (P) http://link.springer.com/chapter/10.1007%2F978-1-4614-6208-8_21 10.1007/978-1-4614-6208-8_21
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Jimat, Dzun Noraini Harwood, Colin Akay, Galip Production of α-amylase by immobilized bacillus subtilis in polymeric polyHIPE matrix
description	The microcellular polymer known as polyHIPE polymer (PHP), with modified physico-chemical characteristics, was developed as a cell matrix for the immobilization of the starch-degrading bacterium, Bacillus subtilis. Suspension of B. subtilis spores was inoculated into a synthesized PHP matrix which is chemically modified, and this PHP matrix was named sulphonated PHPs (SPHPs). These inoculated spores were then activated by supplying continuously well-aerated culture medium (LB medium) and placed in a 37 °C constant temperature room for 24-h incubation. The growth and enzyme productivity data were evaluated and compared. Three different pore and interconnect sizes of SPHPs were evaluated: 42.0 ± 0.61, 36.0 ± 0.50 and 30.0 ± 0.64 μm. The collected samples obtained from the 24-h cultivation were used to determine α-amylase productivity and the loss of cells from the matrices. The morphology, viability and proliferation of the immobilized cells on PHP matrices were observed by scanning electron microscopy (SEM). The SPHP with a pore size of 36.0 μm performed better with respect to the production of α-amylase and the penetration of cells through the whole matrix compared to other SPHPs. The data showed that the total productivity of α-amylase enzyme produced by immobilized cells (on the basis of SPHP volume) was 7.6-fold higher than the batch cell culture. However, if productivity was determined on the basis of used total volume of nutrient medium, that of the immobilized cells was relatively low compared to batch cell culture.
format	Book Chapter
author	Jimat, Dzun Noraini Harwood, Colin Akay, Galip
author_facet	Jimat, Dzun Noraini Harwood, Colin Akay, Galip
author_sort	Jimat, Dzun Noraini
title	Production of α-amylase by immobilized bacillus subtilis in polymeric polyHIPE matrix
title_short	Production of α-amylase by immobilized bacillus subtilis in polymeric polyHIPE matrix
title_full	Production of α-amylase by immobilized bacillus subtilis in polymeric polyHIPE matrix
title_fullStr	Production of α-amylase by immobilized bacillus subtilis in polymeric polyHIPE matrix
title_full_unstemmed	Production of α-amylase by immobilized bacillus subtilis in polymeric polyHIPE matrix
title_sort	production of α-amylase by immobilized bacillus subtilis in polymeric polyhipe matrix
publisher	Springer Science+Business Media
publishDate	2013
url	http://irep.iium.edu.my/34785/ http://irep.iium.edu.my/34785/ http://irep.iium.edu.my/34785/ http://irep.iium.edu.my/34785/1/Jimat.pdf http://irep.iium.edu.my/34785/4/bfm%253A978-1-4614-6208-8%252F1.pdf
first_indexed	2023-09-18T20:50:03Z
last_indexed	2023-09-18T20:50:03Z
_version_	1777409933604552704

Production of α-amylase by immobilized bacillus subtilis in polymeric polyHIPE matrix

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