The cytotoxicity effect of ant-444 in human chronic myeloid leukaemia cells (K 562)

The era of chemotherapy began in the 1940s with the first uses of nitrogen mustards and antifolate drugs. To develop drugs for specific cancer-related targets, researchers from multiple disciplines have joined together in the search for more effective cancer drugs (Chabner & Roberts, 2005). Unfo...

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Main Authors:	Khalid, Nor Bahijah, Abdul Ghani, Radiah
Format:	Conference or Workshop Item
Language:	English English
Published:	2013
Subjects:	RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/34748/ http://irep.iium.edu.my/34748/ http://irep.iium.edu.my/34748/1/MSPP_POSTER.pdf http://irep.iium.edu.my/34748/4/radiah.pdf

id	iium-34748
recordtype	eprints
spelling	iium-347482014-01-24T05:34:12Z http://irep.iium.edu.my/34748/ The cytotoxicity effect of ant-444 in human chronic myeloid leukaemia cells (K 562) Khalid, Nor Bahijah Abdul Ghani, Radiah RM300 Drugs and their action The era of chemotherapy began in the 1940s with the first uses of nitrogen mustards and antifolate drugs. To develop drugs for specific cancer-related targets, researchers from multiple disciplines have joined together in the search for more effective cancer drugs (Chabner & Roberts, 2005). Unfortunately, these chemotherapy drugs are not specific and do not differentiate between killing tumor cells and normal cells, leading to the many common side effects associated with cancer chemotherapy (MacDonald, 2009). Therefore, with the intention of increasing the specificity of anti-cancer drugs targeting as well as decreasing the side effect of the drugs, many studies on polyamine concentration were done since years ago.This project was carried out as a preliminary study with aim to investigate the cytotoxicity effect of a polyamine anthracene conjugate, namely Ant-444 in a human chronic leukemic cell line which is K562. We assessed the cytotoxicity effect of the Ant-444 on the cells by using (3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) colorimetric (MTT) assay and trypan blue dye exclusion method.The result revealed that the drug has the ability to inhibit the growth of cancer cells by showing an IC50 value of 2.4 μM and growth curve results obtained show a significant inhibition of K562 cell growth after been exposed 48 hours to the Ant-444 (96 hour in culture)(P<0.05 at 96 hour and P< 0.001at 120 and 144 hour). In conclusion, the findings obtained confirmed that the drug; Ant-444, has cytotoxic effect against K562 cell line. This basic study will help in providing scientific justification for the use of this polyamine anthracene conjugate in treating cancer. 2013-09 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/34748/1/MSPP_POSTER.pdf application/pdf en http://irep.iium.edu.my/34748/4/radiah.pdf Khalid, Nor Bahijah and Abdul Ghani, Radiah (2013) The cytotoxicity effect of ant-444 in human chronic myeloid leukaemia cells (K 562). In: 27th Scientific Meeting of Malaysian Society of Pharmacology and Physiology (MSPP2013), 6-8 September 2013, MS Garden Hotel, Kuantan. http://mspp2013.com/9-intro/1-mspp-agm.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM300 Drugs and their action
spellingShingle	RM300 Drugs and their action Khalid, Nor Bahijah Abdul Ghani, Radiah The cytotoxicity effect of ant-444 in human chronic myeloid leukaemia cells (K 562)
description	The era of chemotherapy began in the 1940s with the first uses of nitrogen mustards and antifolate drugs. To develop drugs for specific cancer-related targets, researchers from multiple disciplines have joined together in the search for more effective cancer drugs (Chabner & Roberts, 2005). Unfortunately, these chemotherapy drugs are not specific and do not differentiate between killing tumor cells and normal cells, leading to the many common side effects associated with cancer chemotherapy (MacDonald, 2009). Therefore, with the intention of increasing the specificity of anti-cancer drugs targeting as well as decreasing the side effect of the drugs, many studies on polyamine concentration were done since years ago.This project was carried out as a preliminary study with aim to investigate the cytotoxicity effect of a polyamine anthracene conjugate, namely Ant-444 in a human chronic leukemic cell line which is K562. We assessed the cytotoxicity effect of the Ant-444 on the cells by using (3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) colorimetric (MTT) assay and trypan blue dye exclusion method.The result revealed that the drug has the ability to inhibit the growth of cancer cells by showing an IC50 value of 2.4 μM and growth curve results obtained show a significant inhibition of K562 cell growth after been exposed 48 hours to the Ant-444 (96 hour in culture)(P<0.05 at 96 hour and P< 0.001at 120 and 144 hour). In conclusion, the findings obtained confirmed that the drug; Ant-444, has cytotoxic effect against K562 cell line. This basic study will help in providing scientific justification for the use of this polyamine anthracene conjugate in treating cancer.
format	Conference or Workshop Item
author	Khalid, Nor Bahijah Abdul Ghani, Radiah
author_facet	Khalid, Nor Bahijah Abdul Ghani, Radiah
author_sort	Khalid, Nor Bahijah
title	The cytotoxicity effect of ant-444 in human chronic myeloid leukaemia cells (K 562)
title_short	The cytotoxicity effect of ant-444 in human chronic myeloid leukaemia cells (K 562)
title_full	The cytotoxicity effect of ant-444 in human chronic myeloid leukaemia cells (K 562)
title_fullStr	The cytotoxicity effect of ant-444 in human chronic myeloid leukaemia cells (K 562)
title_full_unstemmed	The cytotoxicity effect of ant-444 in human chronic myeloid leukaemia cells (K 562)
title_sort	cytotoxicity effect of ant-444 in human chronic myeloid leukaemia cells (k 562)
publishDate	2013
url	http://irep.iium.edu.my/34748/ http://irep.iium.edu.my/34748/ http://irep.iium.edu.my/34748/1/MSPP_POSTER.pdf http://irep.iium.edu.my/34748/4/radiah.pdf
first_indexed	2023-09-18T20:50:00Z
last_indexed	2023-09-18T20:50:00Z
_version_	1777409930238623744

The cytotoxicity effect of ant-444 in human chronic myeloid leukaemia cells (K 562)

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