Porous plga sheet and acellularized muscle stuffed vein seeded with neural- differentiated mscs are potential scaffolds for nerve regeneration

Porous plga sheet and acellularized muscle stuffed vein seeded with neural- differentiated mscs are potential scaffolds for nerve regeneration

Numerous studies on bridging a nerve gap without using autologous nerve graft have been reported. Our study compares two models of nerve conduit; a porous PLGA sheet and acellular muscle stuffed vein with both models seeded with mesenchymal stem cells. The micro-porous PLGA sheet was fabricated by...

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Main Authors: NH, Hidayah, Sulong, Ahmad Fadzli, Ng, MH, AS, Naicker, A, Shalimar
Format: Article
Language:English
Published: Tissue Engineering and Regenerative Medicine Society of Malaysia 2012
Subjects:
RD701 Orthopedics
Online Access:http://irep.iium.edu.my/34422/
http://irep.iium.edu.my/34422/
http://irep.iium.edu.my/34422/1/TESMA_journal_2012.pdf
id iium-34422
recordtype eprints
spelling iium-344222016-11-04T01:18:09Z http://irep.iium.edu.my/34422/ Porous plga sheet and acellularized muscle stuffed vein seeded with neural- differentiated mscs are potential scaffolds for nerve regeneration NH, Hidayah Sulong, Ahmad Fadzli Ng, MH AS, Naicker A, Shalimar RD701 Orthopedics Numerous studies on bridging a nerve gap without using autologous nerve graft have been reported. Our study compares two models of nerve conduit; a porous PLGA sheet and acellular muscle stuffed vein with both models seeded with mesenchymal stem cells. The micro-porous PLGA sheet was fabricated by solvent casting/salt leaching technique using methylene chloride as the solvent. Both PLGA and muscle stuffed vein was decellularized by liquid nitrogen immersion and the latter further hydrolyzed by soaking in HCl for 24 hours. Neural-differentiated mesenchymal stem cells (MSCs) were seeded and cultured on the surface of both conduits. We studied the seeded constructs for their surface morphology by scanning electron microscope and biocompatibility assessment. Both models of nerve conduit had good distribution and proliferation of seeded cells. For the PLGA sheet, pores had extensive interconnections, excellent for allowing nerve growth. For the muscle stuffed vein, cells were successfully embedded within the hydrolyzed fraction of muscle and ultra structural analysis showed healthy cells attached to the surface of a cellular muscle. In conclusion, both PLGA and muscle stuffed vein seeded with neural-differentiated MSCs are suitable scaffolds for nerve tissue engineering Tissue Engineering and Regenerative Medicine Society of Malaysia 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/34422/1/TESMA_journal_2012.pdf NH, Hidayah and Sulong, Ahmad Fadzli and Ng, MH and AS, Naicker and A, Shalimar (2012) Porous plga sheet and acellularized muscle stuffed vein seeded with neural- differentiated mscs are potential scaffolds for nerve regeneration. Regenerative Research, 1 (1). pp. 1-7. E-ISSN 2232-0822 http://tesma.org.my/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RD701 Orthopedics
spellingShingle RD701 Orthopedics
NH, Hidayah
Sulong, Ahmad Fadzli
Ng, MH
AS, Naicker
A, Shalimar
Porous plga sheet and acellularized muscle stuffed vein seeded with neural- differentiated mscs are potential scaffolds for nerve regeneration
description Numerous studies on bridging a nerve gap without using autologous nerve graft have been reported. Our study compares two models of nerve conduit; a porous PLGA sheet and acellular muscle stuffed vein with both models seeded with mesenchymal stem cells. The micro-porous PLGA sheet was fabricated by solvent casting/salt leaching technique using methylene chloride as the solvent. Both PLGA and muscle stuffed vein was decellularized by liquid nitrogen immersion and the latter further hydrolyzed by soaking in HCl for 24 hours. Neural-differentiated mesenchymal stem cells (MSCs) were seeded and cultured on the surface of both conduits. We studied the seeded constructs for their surface morphology by scanning electron microscope and biocompatibility assessment. Both models of nerve conduit had good distribution and proliferation of seeded cells. For the PLGA sheet, pores had extensive interconnections, excellent for allowing nerve growth. For the muscle stuffed vein, cells were successfully embedded within the hydrolyzed fraction of muscle and ultra structural analysis showed healthy cells attached to the surface of a cellular muscle. In conclusion, both PLGA and muscle stuffed vein seeded with neural-differentiated MSCs are suitable scaffolds for nerve tissue engineering
format Article
author NH, Hidayah
Sulong, Ahmad Fadzli
Ng, MH
AS, Naicker
A, Shalimar
author_facet NH, Hidayah
Sulong, Ahmad Fadzli
Ng, MH
AS, Naicker
A, Shalimar
author_sort NH, Hidayah
title Porous plga sheet and acellularized muscle stuffed vein seeded with neural- differentiated mscs are potential scaffolds for nerve regeneration
title_short Porous plga sheet and acellularized muscle stuffed vein seeded with neural- differentiated mscs are potential scaffolds for nerve regeneration
title_full Porous plga sheet and acellularized muscle stuffed vein seeded with neural- differentiated mscs are potential scaffolds for nerve regeneration
title_fullStr Porous plga sheet and acellularized muscle stuffed vein seeded with neural- differentiated mscs are potential scaffolds for nerve regeneration
title_full_unstemmed Porous plga sheet and acellularized muscle stuffed vein seeded with neural- differentiated mscs are potential scaffolds for nerve regeneration
title_sort porous plga sheet and acellularized muscle stuffed vein seeded with neural- differentiated mscs are potential scaffolds for nerve regeneration
publisher Tissue Engineering and Regenerative Medicine Society of Malaysia
publishDate 2012
url http://irep.iium.edu.my/34422/
http://irep.iium.edu.my/34422/
http://irep.iium.edu.my/34422/1/TESMA_journal_2012.pdf
first_indexed 2023-09-18T20:49:37Z
last_indexed 2023-09-18T20:49:37Z
_version_ 1777409906020712448

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