Effects of thymoquinone supplementation on cyclophosphamide toxicity of mouse embryo in vitro

Thymoquinone is the major active component derived from the traditional medicinal plant Nigella sativa, which has been shown to exhibit antioxidant property through different mechanisms in animal models. This study evaluates the prophylactic effect of thymoquinone supplementation on culture medium...

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Main Authors:	Abdul Rahman, Suzanah, Kamarzaman, Saheera, Abdul Wahab, Azantee Yazmie
Format:	Article
Language:	English English
Published:	IDOSI Publications 2014
Subjects:	QP Physiology R Medicine (General)
Online Access:	http://irep.iium.edu.my/34217/ http://irep.iium.edu.my/34217/ http://irep.iium.edu.my/34217/1/Latest_GV_PDF.pdf http://irep.iium.edu.my/34217/4/34217_Effects%20of%20thymoquinone_SCOPUS.pdf

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recordtype	eprints
spelling	iium-342172018-06-29T03:12:52Z http://irep.iium.edu.my/34217/ Effects of thymoquinone supplementation on cyclophosphamide toxicity of mouse embryo in vitro Abdul Rahman, Suzanah Kamarzaman, Saheera Abdul Wahab, Azantee Yazmie QP Physiology R Medicine (General) Thymoquinone is the major active component derived from the traditional medicinal plant Nigella sativa, which has been shown to exhibit antioxidant property through different mechanisms in animal models. This study evaluates the prophylactic effect of thymoquinone supplementation on culture medium to ameliorate cyclophosphamide-induced alterations in cellular differentiation and proliferation during embryo development in vitro. Male and female mice were exposed to cyclophosphamide via a single intraperitoneal (i.p.) injection at 200 mg/kg. Sperms and oocytes were collected at day 33 and day 10 respectively, for insemination and fertilization in medium supplemented with thymoquinone(1μM, 10μM and 100μM). The stages of fertilization, embryo division, morphological effects and fragmentation were examined and compared between groups, 24 hours post-fertilization. Thymoquinone supplementation improved fertilization rates, significantly reduced the percentage of defects blastomeres of Type C (p<0.001) and significantly decreased the percentage of embryo fragmentation Grade IV (>50%, p<0.05) following paternal and maternal exposures to cyclophosphamide. The good quality embryos of Type A and Grade I fragmentation were not observed in the group without thymoquinone supplementation. The findings of this study showed that thymoquinone is a suitable exogenous antioxidant for preserving fair-quality embryos which can result into full term pregnancy. IDOSI Publications 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/34217/1/Latest_GV_PDF.pdf application/pdf en http://irep.iium.edu.my/34217/4/34217_Effects%20of%20thymoquinone_SCOPUS.pdf Abdul Rahman, Suzanah and Kamarzaman, Saheera and Abdul Wahab, Azantee Yazmie (2014) Effects of thymoquinone supplementation on cyclophosphamide toxicity of mouse embryo in vitro. Global Veterinaria, 12 (1). pp. 80-90. ISSN 1992-6197 http://www.idosi.org/gv/online.htm
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QP Physiology R Medicine (General)
spellingShingle	QP Physiology R Medicine (General) Abdul Rahman, Suzanah Kamarzaman, Saheera Abdul Wahab, Azantee Yazmie Effects of thymoquinone supplementation on cyclophosphamide toxicity of mouse embryo in vitro
description	Thymoquinone is the major active component derived from the traditional medicinal plant Nigella sativa, which has been shown to exhibit antioxidant property through different mechanisms in animal models. This study evaluates the prophylactic effect of thymoquinone supplementation on culture medium to ameliorate cyclophosphamide-induced alterations in cellular differentiation and proliferation during embryo development in vitro. Male and female mice were exposed to cyclophosphamide via a single intraperitoneal (i.p.) injection at 200 mg/kg. Sperms and oocytes were collected at day 33 and day 10 respectively, for insemination and fertilization in medium supplemented with thymoquinone(1μM, 10μM and 100μM). The stages of fertilization, embryo division, morphological effects and fragmentation were examined and compared between groups, 24 hours post-fertilization. Thymoquinone supplementation improved fertilization rates, significantly reduced the percentage of defects blastomeres of Type C (p<0.001) and significantly decreased the percentage of embryo fragmentation Grade IV (>50%, p<0.05) following paternal and maternal exposures to cyclophosphamide. The good quality embryos of Type A and Grade I fragmentation were not observed in the group without thymoquinone supplementation. The findings of this study showed that thymoquinone is a suitable exogenous antioxidant for preserving fair-quality embryos which can result into full term pregnancy.
format	Article
author	Abdul Rahman, Suzanah Kamarzaman, Saheera Abdul Wahab, Azantee Yazmie
author_facet	Abdul Rahman, Suzanah Kamarzaman, Saheera Abdul Wahab, Azantee Yazmie
author_sort	Abdul Rahman, Suzanah
title	Effects of thymoquinone supplementation on cyclophosphamide toxicity of mouse embryo in vitro
title_short	Effects of thymoquinone supplementation on cyclophosphamide toxicity of mouse embryo in vitro
title_full	Effects of thymoquinone supplementation on cyclophosphamide toxicity of mouse embryo in vitro
title_fullStr	Effects of thymoquinone supplementation on cyclophosphamide toxicity of mouse embryo in vitro
title_full_unstemmed	Effects of thymoquinone supplementation on cyclophosphamide toxicity of mouse embryo in vitro
title_sort	effects of thymoquinone supplementation on cyclophosphamide toxicity of mouse embryo in vitro
publisher	IDOSI Publications
publishDate	2014
url	http://irep.iium.edu.my/34217/ http://irep.iium.edu.my/34217/ http://irep.iium.edu.my/34217/1/Latest_GV_PDF.pdf http://irep.iium.edu.my/34217/4/34217_Effects%20of%20thymoquinone_SCOPUS.pdf
first_indexed	2023-09-18T20:49:22Z
last_indexed	2023-09-18T20:49:22Z
_version_	1777409890825797632

Effects of thymoquinone supplementation on cyclophosphamide toxicity of mouse embryo in vitro

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