Transition state analysis of Pyranose 2-Oxidase from Peniophora sp.

Significant interest has been aroused for the use of pyranose 2-oxidase (P2Ox) as a catalyst in biotechnological applications particularly in biofuel and food technology. Understanding the transition state of this enzyme offers greater possibility to improve the catalytic activity of this enzyme...

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Main Authors:	Ibrahim Ali , Noorbatcha, Ramli, Siti Khairani, Salleh, Hamzah Mohd.
Format:	Conference or Workshop Item
Language:	English English English
Published:	2013
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/34141/ http://irep.iium.edu.my/34141/5/ICBIOE_2013_Cover_Page_till_Table_of_contents.pdf http://irep.iium.edu.my/34141/1/TST_P2Ox_-_ICBioE2013_proceedings_paper_pp274-277-.pdf http://irep.iium.edu.my/34141/4/Brochure.pdf

id	iium-34141
recordtype	eprints
spelling	iium-341412014-01-15T02:54:37Z http://irep.iium.edu.my/34141/ Transition state analysis of Pyranose 2-Oxidase from Peniophora sp. Ibrahim Ali , Noorbatcha Ramli, Siti Khairani Salleh, Hamzah Mohd. TP248.13 Biotechnology Significant interest has been aroused for the use of pyranose 2-oxidase (P2Ox) as a catalyst in biotechnological applications particularly in biofuel and food technology. Understanding the transition state of this enzyme offers greater possibility to improve the catalytic activity of this enzyme and thus speeding the industrial processes. The main purpose of this study is to identify and characterize the transition states for the catalytic reaction of P2Ox from white-rot fungus Peniophora sp.. The complete path for the oxidation reaction catalyzed by P2Ox was investigated using the PM7 semi-empirical method with MOZYME function as implemented in the MOPAC 2012 (Molecular Orbital PACkage) program. The results of this study was consistent with the experimental method where appreciable conformational changes can be seen in the loop of residues 454-461 which was anticipated to be functions as a gatekeeper to allow the substrate to pass into the active center. Keywords: pyranose-2-oxidase, fungus, transition state, PM7, MOZYME 2013-07-02 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/34141/5/ICBIOE_2013_Cover_Page_till_Table_of_contents.pdf application/pdf en http://irep.iium.edu.my/34141/1/TST_P2Ox_-_ICBioE2013_proceedings_paper_pp274-277-.pdf application/pdf en http://irep.iium.edu.my/34141/4/Brochure.pdf Ibrahim Ali , Noorbatcha and Ramli, Siti Khairani and Salleh, Hamzah Mohd. (2013) Transition state analysis of Pyranose 2-Oxidase from Peniophora sp. In: International Conference on Biotechnology Engineering (ICBioE2013), 2-4 July 2013, Kuala Lumpur, Malaysia.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ibrahim Ali , Noorbatcha Ramli, Siti Khairani Salleh, Hamzah Mohd. Transition state analysis of Pyranose 2-Oxidase from Peniophora sp.
description	Significant interest has been aroused for the use of pyranose 2-oxidase (P2Ox) as a catalyst in biotechnological applications particularly in biofuel and food technology. Understanding the transition state of this enzyme offers greater possibility to improve the catalytic activity of this enzyme and thus speeding the industrial processes. The main purpose of this study is to identify and characterize the transition states for the catalytic reaction of P2Ox from white-rot fungus Peniophora sp.. The complete path for the oxidation reaction catalyzed by P2Ox was investigated using the PM7 semi-empirical method with MOZYME function as implemented in the MOPAC 2012 (Molecular Orbital PACkage) program. The results of this study was consistent with the experimental method where appreciable conformational changes can be seen in the loop of residues 454-461 which was anticipated to be functions as a gatekeeper to allow the substrate to pass into the active center. Keywords: pyranose-2-oxidase, fungus, transition state, PM7, MOZYME
format	Conference or Workshop Item
author	Ibrahim Ali , Noorbatcha Ramli, Siti Khairani Salleh, Hamzah Mohd.
author_facet	Ibrahim Ali , Noorbatcha Ramli, Siti Khairani Salleh, Hamzah Mohd.
author_sort	Ibrahim Ali , Noorbatcha
title	Transition state analysis of Pyranose 2-Oxidase from Peniophora sp.
title_short	Transition state analysis of Pyranose 2-Oxidase from Peniophora sp.
title_full	Transition state analysis of Pyranose 2-Oxidase from Peniophora sp.
title_fullStr	Transition state analysis of Pyranose 2-Oxidase from Peniophora sp.
title_full_unstemmed	Transition state analysis of Pyranose 2-Oxidase from Peniophora sp.
title_sort	transition state analysis of pyranose 2-oxidase from peniophora sp.
publishDate	2013
url	http://irep.iium.edu.my/34141/ http://irep.iium.edu.my/34141/5/ICBIOE_2013_Cover_Page_till_Table_of_contents.pdf http://irep.iium.edu.my/34141/1/TST_P2Ox_-_ICBioE2013_proceedings_paper_pp274-277-.pdf http://irep.iium.edu.my/34141/4/Brochure.pdf
first_indexed	2023-09-18T20:49:15Z
last_indexed	2023-09-18T20:49:15Z
_version_	1777409883710160896

Transition state analysis of Pyranose 2-Oxidase from Peniophora sp.

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