Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
The green synthesis of nanoparticles that have environmentally acceptable solvent systems and ecofriendly reducing agents is of great importance. Meanwhile, the synthesis of metal nanoparticles of different sizes, shapes, chemical composition and controlled monodispersity is a key area of researc...
| Main Authors: | , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English English English |
| Published: |
2013
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/34114/ http://irep.iium.edu.my/34114/3/ICBIOE_2013_Cover_Page_till_Table_of_contents.pdf http://irep.iium.edu.my/34114/1/AuNP_-_ICBioE2013_proceedings_paper_pp270-273-.pdf http://irep.iium.edu.my/34114/2/Brochure.pdf |
| Summary: | The green synthesis of nanoparticles that have environmentally acceptable solvent systems and ecofriendly
reducing agents is of great importance. Meanwhile, the synthesis of metal nanoparticles of
different sizes, shapes, chemical composition and controlled monodispersity is a key area of
research in nanotechnology because of their fascinating physical properties and technological
applications. As such, biosynthesis of gold nanoparticles with small size and biostability is vital and
used in various biomedical applications. In this paper we report the extracellular biosynthesis of
gold nanoparticles (AuNPs) by using Candida cylindracea (also known as Candida rugosa), yeast.
In the biosynthesis of AuNPs using this yeast, the cultured yeast was exposed to the chloroauric
trihydrate. The bioreduction process involves Au+ ions which are reduced to metallic AuNPs
through the catalytic effect of the extracellular enzyme. These gold nanoparticles were
characterized by the means of UV–Vis spectroscopy, transmission electron microscopy (TEM), Xray
Diffraction spectrum (XRD) and Fourier transform infrared spectroscopy (FTIR). UV–visible
spectrum of the aqueous medium containing auric ion showed a peak at 530 nm corresponding to
the surface plasmon resonance of gold nanoparticles. The intensity of the colour was found to be
increases with respect of time. TEM micrograph showed the formation of well-dispersed gold
nanoparticles in the range of 10–30 nm with spherical and triangular shape. Fourier transform
infrared spectroscopy revealed possible involvement of reductive groups on the surfaces of
nanoparticles. Hence the present study enlightens the green chemistry approach on the production
of gold nanoparticles using a microorganism. In comparison to chemical synthesis, the synthesis of
gold nanoparticles by microbial source is the most reliable method of production and yield. |
|---|