Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells

Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells

ABCG2 is a transporter preferentially expressed in a primitive subpopulation of cells and recently reported as a surviving factor for trophoblasts. To date, manner of ABCG2 expression in lymphoid tissues is not known. Immunohistochemically, strong ABCG2 expression was found in a small proportion of...

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Main Authors: Nakamichi, Naoto, Morii, Eiichi, Ikeda, Jun-Ichiro, Qiu, Ying, Mamat, Suhana, Tian, Tian, Fukuhara, Shirou, Aozasa, Katsuyuki
Format: Article
Language:English
Published: Nature Publishing Group 2009
Subjects:
RB Pathology
Online Access:http://irep.iium.edu.my/34105/
http://irep.iium.edu.my/34105/
http://irep.iium.edu.my/34105/
http://irep.iium.edu.my/34105/1/2009.pdf
id iium-34105
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spelling iium-341052014-01-13T06:24:45Z http://irep.iium.edu.my/34105/ Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells Nakamichi, Naoto Morii, Eiichi Ikeda, Jun-Ichiro Qiu, Ying Mamat, Suhana Tian, Tian Fukuhara, Shirou Aozasa, Katsuyuki RB Pathology ABCG2 is a transporter preferentially expressed in a primitive subpopulation of cells and recently reported as a surviving factor for trophoblasts. To date, manner of ABCG2 expression in lymphoid tissues is not known. Immunohistochemically, strong ABCG2 expression was found in a small proportion of plasma cells mainly located in the interfollicular space of lymphoid tissues. The number of ABCG2-high plasma cells increased in interleukin-6- (IL-6) rich lesions, such as Castleman's disease of plasma cell type. Plasma cells are subjected to endoplasmic reticulum (ER) stress when excess proteins are synthesized, and IL-6 stimulates protein synthesis. Therefore, the effect of IL-6 and ER stress on ABCG2 expression in plasma cells was examined. The expression level of ABCG2 increased by treatment with either IL-6 or ER stress inducers, and further increased with both. The promoter analysis revealed that the effect of IL-6 and ER stress inducers was mediated through the site overlapping XBP-1 and HIF-1 binding sequences. Knocked-down of ABCG2 by siRNA or ABCG2 inhibitor reduced plasma cell viability under ER stress. These suggest that ABCG2 is a surviving factor for plasma cells. To our knowledge, this is the first study reporting the effect of ER stress on ABCG2 expression. Nature Publishing Group 2009-01-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/34105/1/2009.pdf Nakamichi, Naoto and Morii, Eiichi and Ikeda, Jun-Ichiro and Qiu, Ying and Mamat, Suhana and Tian, Tian and Fukuhara, Shirou and Aozasa, Katsuyuki (2009) Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells. Laboratory Investigation, 89 (3). pp. 327-336. ISSN 1530-0307 (O), 0023-6837 (P) http://www.nature.com/labinvest/journal/v89/n3/full/labinvest2008157a.html 10.1038/labinvest.2008.157
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RB Pathology
spellingShingle RB Pathology
Nakamichi, Naoto
Morii, Eiichi
Ikeda, Jun-Ichiro
Qiu, Ying
Mamat, Suhana
Tian, Tian
Fukuhara, Shirou
Aozasa, Katsuyuki
Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells
description ABCG2 is a transporter preferentially expressed in a primitive subpopulation of cells and recently reported as a surviving factor for trophoblasts. To date, manner of ABCG2 expression in lymphoid tissues is not known. Immunohistochemically, strong ABCG2 expression was found in a small proportion of plasma cells mainly located in the interfollicular space of lymphoid tissues. The number of ABCG2-high plasma cells increased in interleukin-6- (IL-6) rich lesions, such as Castleman's disease of plasma cell type. Plasma cells are subjected to endoplasmic reticulum (ER) stress when excess proteins are synthesized, and IL-6 stimulates protein synthesis. Therefore, the effect of IL-6 and ER stress on ABCG2 expression in plasma cells was examined. The expression level of ABCG2 increased by treatment with either IL-6 or ER stress inducers, and further increased with both. The promoter analysis revealed that the effect of IL-6 and ER stress inducers was mediated through the site overlapping XBP-1 and HIF-1 binding sequences. Knocked-down of ABCG2 by siRNA or ABCG2 inhibitor reduced plasma cell viability under ER stress. These suggest that ABCG2 is a surviving factor for plasma cells. To our knowledge, this is the first study reporting the effect of ER stress on ABCG2 expression.
format Article
author Nakamichi, Naoto
Morii, Eiichi
Ikeda, Jun-Ichiro
Qiu, Ying
Mamat, Suhana
Tian, Tian
Fukuhara, Shirou
Aozasa, Katsuyuki
author_facet Nakamichi, Naoto
Morii, Eiichi
Ikeda, Jun-Ichiro
Qiu, Ying
Mamat, Suhana
Tian, Tian
Fukuhara, Shirou
Aozasa, Katsuyuki
author_sort Nakamichi, Naoto
title Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells
title_short Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells
title_full Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells
title_fullStr Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells
title_full_unstemmed Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells
title_sort synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of abcg2 expression in plasma cells
publisher Nature Publishing Group
publishDate 2009
url http://irep.iium.edu.my/34105/
http://irep.iium.edu.my/34105/
http://irep.iium.edu.my/34105/
http://irep.iium.edu.my/34105/1/2009.pdf
first_indexed 2023-09-18T20:49:12Z
last_indexed 2023-09-18T20:49:12Z
_version_ 1777409880629444608

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