Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells

Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells

Tumor cells with tumorigenic potential might be limited to a small population of cells, called cancer-initiating cells (CICs). CICs efficiently form colonies in vitro, yield both CIC and non-CIC populations, maintain reactive oxygen species (ROS) at low levels, show high aldehyde dehydrogenase (ALDH...

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Main Authors: Ikeda, Jun-ichiro, Mamat, Suhana, Tian, Tian, Wang, Yi, Luo, Wenjuan, Rahadiani, Nur, Aozasa, Katsuyuki, Morii, Eiichi
Format: Article
Language:English
Published: Nature Publishing Group 2012
Subjects:
RB Pathology
Online Access:http://irep.iium.edu.my/34103/
http://irep.iium.edu.my/34103/
http://irep.iium.edu.my/34103/1/2012.pdf
id iium-34103
recordtype eprints
spelling iium-341032014-01-10T08:30:53Z http://irep.iium.edu.my/34103/ Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells Ikeda, Jun-ichiro Mamat, Suhana Tian, Tian Wang, Yi Luo, Wenjuan Rahadiani, Nur Aozasa, Katsuyuki Morii, Eiichi RB Pathology Tumor cells with tumorigenic potential might be limited to a small population of cells, called cancer-initiating cells (CICs). CICs efficiently form colonies in vitro, yield both CIC and non-CIC populations, maintain reactive oxygen species (ROS) at low levels, show high aldehyde dehydrogenase (ALDH) activity, and are mostly in a quiescent state of the cell cycle. CICs of Hodgkin lymphoma (HL) are small in size, with low levels of ROS. The relationship between ROS level and ALDH activity in CICs was examined in HL cell lines. ROS-low and ALDH-high populations formed colonies in semi-solid cultures more efficiently than ROS-high and ALDH-low populations. ALDH-high populations yielded both ALDH-low and -high populations, whereas ALDH-low populations rarely yielded an ALDH-high population. The number of cells in a quiescent state was significantly greater in ROS-low than in ROS-high cells, whereas that of ALDH-high and ALDH-low cells was comparable to each other. These findings show that ALDH-high and ROS-low cells share CIC-like potential, but they differ in their cell cycle status, suggesting that CICs are comprised of cells with heterogeneous characteristics. Nature Publishing Group 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/34103/1/2012.pdf Ikeda, Jun-ichiro and Mamat, Suhana and Tian, Tian and Wang, Yi and Luo, Wenjuan and Rahadiani, Nur and Aozasa, Katsuyuki and Morii, Eiichi (2012) Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells. Laboratory Investigation, 92 (4). pp. 606-6014. ISSN 1530-0307 (E-ISSN), 0023-6837 (ISSN) doi:10.1038/labinvest.2012.4
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RB Pathology
spellingShingle RB Pathology
Ikeda, Jun-ichiro
Mamat, Suhana
Tian, Tian
Wang, Yi
Luo, Wenjuan
Rahadiani, Nur
Aozasa, Katsuyuki
Morii, Eiichi
Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells
description Tumor cells with tumorigenic potential might be limited to a small population of cells, called cancer-initiating cells (CICs). CICs efficiently form colonies in vitro, yield both CIC and non-CIC populations, maintain reactive oxygen species (ROS) at low levels, show high aldehyde dehydrogenase (ALDH) activity, and are mostly in a quiescent state of the cell cycle. CICs of Hodgkin lymphoma (HL) are small in size, with low levels of ROS. The relationship between ROS level and ALDH activity in CICs was examined in HL cell lines. ROS-low and ALDH-high populations formed colonies in semi-solid cultures more efficiently than ROS-high and ALDH-low populations. ALDH-high populations yielded both ALDH-low and -high populations, whereas ALDH-low populations rarely yielded an ALDH-high population. The number of cells in a quiescent state was significantly greater in ROS-low than in ROS-high cells, whereas that of ALDH-high and ALDH-low cells was comparable to each other. These findings show that ALDH-high and ROS-low cells share CIC-like potential, but they differ in their cell cycle status, suggesting that CICs are comprised of cells with heterogeneous characteristics.
format Article
author Ikeda, Jun-ichiro
Mamat, Suhana
Tian, Tian
Wang, Yi
Luo, Wenjuan
Rahadiani, Nur
Aozasa, Katsuyuki
Morii, Eiichi
author_facet Ikeda, Jun-ichiro
Mamat, Suhana
Tian, Tian
Wang, Yi
Luo, Wenjuan
Rahadiani, Nur
Aozasa, Katsuyuki
Morii, Eiichi
author_sort Ikeda, Jun-ichiro
title Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells
title_short Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells
title_full Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells
title_fullStr Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells
title_full_unstemmed Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells
title_sort reactive oxygen species and aldehyde dehydrogenase activity in hodgkin lymphoma cells
publisher Nature Publishing Group
publishDate 2012
url http://irep.iium.edu.my/34103/
http://irep.iium.edu.my/34103/
http://irep.iium.edu.my/34103/1/2012.pdf
first_indexed 2023-09-18T20:49:12Z
last_indexed 2023-09-18T20:49:12Z
_version_ 1777409880330600448

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