Cloning and expression of Voltage-dependent Anion Channel 2 (VDAC2) from electrically stimulated chicken
VDAC2 protein identified to be overexpressed in stunned chicken is a potential biomarker in differentiating stunned chicken since the expression was proportional to the voltage applied. To facilitate research, the gene encoding VDAC is cloned, expressed and optimized in BL21-AI Escherichia coli....
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/34096/ http://irep.iium.edu.my/34096/ http://irep.iium.edu.my/34096/1/JPAM_VDAC2.pdf |
| Summary: | VDAC2 protein identified to be overexpressed in stunned chicken is a potential
biomarker in differentiating stunned chicken since the expression was proportional to
the voltage applied. To facilitate research, the gene encoding VDAC is cloned, expressed
and optimized in BL21-AI Escherichia coli. cDNA was synthesized from mRNA of stunned
chicken’s skeletal muscle and cloned using recombination reaction. All positive clones
were identified by PCR and restriction enzyme digestion as well as DNA sequencing.
Recombinant VDAC2 protein was purified by Ni-NTA spin-column. Western blot performed
using polyclonal anti-N terminal human VDAC2 and anti-His tag revealed a 34 kDa
protein, confirming the expression of recombinant VDAC2. |
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