Thymoquinone, an active ingredient of Black Seed (Nigela saliva) induces distinct apoptotic pathways in C33A and Siha Cervical Cancer Cell Lines)

Thymoquinone (TQ) is the main constituent of black seed (Nigella sativa, spp) essential oil, which shows promising in vitro and in vivo anti-neoplastic activities against different tumor cell lines. However, the apoptotic effects of TQ and how TQ induces apoptosis in cervical cancer cells still rema...

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Main Authors:	Ichwan, Solachuddin JA, Al-Ani, Imad Matloub Dally, Suriyah, Wastuti, Muhammad, Taher
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Subjects:	RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/33810/ http://irep.iium.edu.my/33810/ http://irep.iium.edu.my/33810/1/TCAM_2012.pdf

id	iium-33810
recordtype	eprints
spelling	iium-338102015-12-18T03:04:12Z http://irep.iium.edu.my/33810/ Thymoquinone, an active ingredient of Black Seed (Nigela saliva) induces distinct apoptotic pathways in C33A and Siha Cervical Cancer Cell Lines) Ichwan, Solachuddin JA Al-Ani, Imad Matloub Dally Suriyah, Wastuti Muhammad, Taher RM300 Drugs and their action Thymoquinone (TQ) is the main constituent of black seed (Nigella sativa, spp) essential oil, which shows promising in vitro and in vivo anti-neoplastic activities against different tumor cell lines. However, the apoptotic effects of TQ and how TQ induces apoptosis in cervical cancer cells still remains to be clarified. Cervical cancers have been generally treated by combination of surgery and/or chemo-radiotherapy. However that is not applicable to a later stage of the cancer whereby only palliative treatment is available with many side effects. p53 gene is a specific gene that functions to suppress the growth of tumors. The gene is frequently lost or inactivated in cervical cancers. This research was aimed to explore the apoptotic mechanism of TQ in inducing apoptosis on two different human cervical cell lines; Siha and C33A cells. The cell lines harbor distinct non-functional p53 tumor suppressor gene. Although p53 is wild-type in Siha, the cells expressing E6 oncoprotein that diminishing the endogenous p53 function, whereas in C33A cells, the p53 is mutated. We found that TQ stimulates distinct apoptotic pathway in Siha and C33A cells. TQ markedly induced apoptosis as demonstrated by DNA fragmentation assay in both cell lines. Moreover, we demonstrated for the first time that TQ-induced apoptosis in Siha cells through p53-dependent pathway as shown by elevated level of p53-mediated apoptosis target genes as shown by Quantitative PCR analysis, whereas in C33A cells is mainly associated with the activation of Caspase-3. These results support further research in exploring the potential clinical usefulness of TQ for cervical cancers treatment. 2012-06-22 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/33810/1/TCAM_2012.pdf Ichwan, Solachuddin JA and Al-Ani, Imad Matloub Dally and Suriyah, Wastuti and Muhammad, Taher (2012) Thymoquinone, an active ingredient of Black Seed (Nigela saliva) induces distinct apoptotic pathways in C33A and Siha Cervical Cancer Cell Lines). In: International Conference Research and Application on Traditional Complementary and Alternative Medicine in Health Care (TCAM 2012), 22-23 June 2012, Surakarta, Indonesia. (Unpublished) http://tcam-ums.com
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM300 Drugs and their action
spellingShingle	RM300 Drugs and their action Ichwan, Solachuddin JA Al-Ani, Imad Matloub Dally Suriyah, Wastuti Muhammad, Taher Thymoquinone, an active ingredient of Black Seed (Nigela saliva) induces distinct apoptotic pathways in C33A and Siha Cervical Cancer Cell Lines)
description	Thymoquinone (TQ) is the main constituent of black seed (Nigella sativa, spp) essential oil, which shows promising in vitro and in vivo anti-neoplastic activities against different tumor cell lines. However, the apoptotic effects of TQ and how TQ induces apoptosis in cervical cancer cells still remains to be clarified. Cervical cancers have been generally treated by combination of surgery and/or chemo-radiotherapy. However that is not applicable to a later stage of the cancer whereby only palliative treatment is available with many side effects. p53 gene is a specific gene that functions to suppress the growth of tumors. The gene is frequently lost or inactivated in cervical cancers. This research was aimed to explore the apoptotic mechanism of TQ in inducing apoptosis on two different human cervical cell lines; Siha and C33A cells. The cell lines harbor distinct non-functional p53 tumor suppressor gene. Although p53 is wild-type in Siha, the cells expressing E6 oncoprotein that diminishing the endogenous p53 function, whereas in C33A cells, the p53 is mutated. We found that TQ stimulates distinct apoptotic pathway in Siha and C33A cells. TQ markedly induced apoptosis as demonstrated by DNA fragmentation assay in both cell lines. Moreover, we demonstrated for the first time that TQ-induced apoptosis in Siha cells through p53-dependent pathway as shown by elevated level of p53-mediated apoptosis target genes as shown by Quantitative PCR analysis, whereas in C33A cells is mainly associated with the activation of Caspase-3. These results support further research in exploring the potential clinical usefulness of TQ for cervical cancers treatment.
format	Conference or Workshop Item
author	Ichwan, Solachuddin JA Al-Ani, Imad Matloub Dally Suriyah, Wastuti Muhammad, Taher
author_facet	Ichwan, Solachuddin JA Al-Ani, Imad Matloub Dally Suriyah, Wastuti Muhammad, Taher
author_sort	Ichwan, Solachuddin JA
title	Thymoquinone, an active ingredient of Black Seed (Nigela saliva) induces distinct apoptotic pathways in C33A and Siha Cervical Cancer Cell Lines)
title_short	Thymoquinone, an active ingredient of Black Seed (Nigela saliva) induces distinct apoptotic pathways in C33A and Siha Cervical Cancer Cell Lines)
title_full	Thymoquinone, an active ingredient of Black Seed (Nigela saliva) induces distinct apoptotic pathways in C33A and Siha Cervical Cancer Cell Lines)
title_fullStr	Thymoquinone, an active ingredient of Black Seed (Nigela saliva) induces distinct apoptotic pathways in C33A and Siha Cervical Cancer Cell Lines)
title_full_unstemmed	Thymoquinone, an active ingredient of Black Seed (Nigela saliva) induces distinct apoptotic pathways in C33A and Siha Cervical Cancer Cell Lines)
title_sort	thymoquinone, an active ingredient of black seed (nigela saliva) induces distinct apoptotic pathways in c33a and siha cervical cancer cell lines)
publishDate	2012
url	http://irep.iium.edu.my/33810/ http://irep.iium.edu.my/33810/ http://irep.iium.edu.my/33810/1/TCAM_2012.pdf
first_indexed	2023-09-18T20:48:54Z
last_indexed	2023-09-18T20:48:54Z
_version_	1777409861412192256

Thymoquinone, an active ingredient of Black Seed (Nigela saliva) induces distinct apoptotic pathways in C33A and Siha Cervical Cancer Cell Lines)

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