The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study

Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the teste...

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Main Authors:	Al-Ani, Imad Matloub Dally, Salman , Mohammad Oda, Al-Wasiti , Estabraq A., Thamir, Khalid T., Al-Salihi, Anam R.
Format:	Conference or Workshop Item
Language:	English
Published:	2013
Subjects:	QM Human anatomy QP Physiology RB Pathology
Online Access:	http://irep.iium.edu.my/33510/ http://irep.iium.edu.my/33510/1/12MSM_2.jpg

id	iium-33510
recordtype	eprints
spelling	iium-335102015-12-18T02:41:50Z http://irep.iium.edu.my/33510/ The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study Al-Ani, Imad Matloub Dally Salman , Mohammad Oda Al-Wasiti , Estabraq A. Thamir, Khalid T. Al-Salihi, Anam R. QM Human anatomy QP Physiology RB Pathology Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the testes were evaluated for histological changes and DNA fragmentation by single cell gel electrophoresis (comet assay). Results: Marked alterations were observed in the testes of vasectomized mice, including degeneration of spermatids, thickened basement membrane, dilatation of the seminiferous tubules, exfoliation of germ cells, reduction in the seminiferous cell population, vacuolated appearance of the epithelium in the tubules and marked interstitial fibrosis. Single cell gel electrophoresis showed a highly significant (P<0.0001)increase in DNA damage among vasectomized mice (46.02%) compared with control group (%27.17)after six weeks of operation. Conclusion: Vasectomy induced deterioration in the seminiferous tubules associated with increased testicular cell’s DNA fragmentation. KEYWORDS: Vasectomy, comet assay, DNA degeneration, vas deferens. 2013 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/33510/1/12MSM_2.jpg Al-Ani, Imad Matloub Dally and Salman , Mohammad Oda and Al-Wasiti , Estabraq A. and Thamir, Khalid T. and Al-Salihi, Anam R. (2013) The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study. In: 22nd Scientific Conference of the Microscopy Society Malaysia, 26-28 Nov 2013, Primula Beach Hotel, Terengganu .
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QM Human anatomy QP Physiology RB Pathology
spellingShingle	QM Human anatomy QP Physiology RB Pathology Al-Ani, Imad Matloub Dally Salman , Mohammad Oda Al-Wasiti , Estabraq A. Thamir, Khalid T. Al-Salihi, Anam R. The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study
description	Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the testes were evaluated for histological changes and DNA fragmentation by single cell gel electrophoresis (comet assay). Results: Marked alterations were observed in the testes of vasectomized mice, including degeneration of spermatids, thickened basement membrane, dilatation of the seminiferous tubules, exfoliation of germ cells, reduction in the seminiferous cell population, vacuolated appearance of the epithelium in the tubules and marked interstitial fibrosis. Single cell gel electrophoresis showed a highly significant (P<0.0001)increase in DNA damage among vasectomized mice (46.02%) compared with control group (%27.17)after six weeks of operation. Conclusion: Vasectomy induced deterioration in the seminiferous tubules associated with increased testicular cell’s DNA fragmentation. KEYWORDS: Vasectomy, comet assay, DNA degeneration, vas deferens.
format	Conference or Workshop Item
author	Al-Ani, Imad Matloub Dally Salman , Mohammad Oda Al-Wasiti , Estabraq A. Thamir, Khalid T. Al-Salihi, Anam R.
author_facet	Al-Ani, Imad Matloub Dally Salman , Mohammad Oda Al-Wasiti , Estabraq A. Thamir, Khalid T. Al-Salihi, Anam R.
author_sort	Al-Ani, Imad Matloub Dally
title	The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study
title_short	The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study
title_full	The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study
title_fullStr	The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study
title_full_unstemmed	The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study
title_sort	effects of vasectomy on testicular tissue of mice: histological changes and dna fragmentation study
publishDate	2013
url	http://irep.iium.edu.my/33510/ http://irep.iium.edu.my/33510/1/12MSM_2.jpg
first_indexed	2023-09-18T20:48:26Z
last_indexed	2023-09-18T20:48:26Z
_version_	1777409832483028992

The effects of vasectomy on testicular tissue of mice: Histological changes and DNA fragmentation study

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