Metabolic changes in different developmental stages of vanilla planifolia pods

The metabolomic analysis of developing Vanilla planifolia green pods (between 3 and 8 months after pollination) was carried out by nuclear magnetic resonance (NMR) spectroscopy and multivariate data analysis. Multivariate data analysis of the 1H NMR spectra, such as principal component analysis (PCA...

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Main Authors:	Palama, Tony Lionel, Khatib, Alfi, Choi, Young Hae, Payet, Bertrand, Fock, Isabelle, Verpoorte, Robert, Kodja, Hippolyte
Format:	Article
Language:	English
Published:	American Chemical Society 2009
Subjects:	S Agriculture (General)
Online Access:	http://irep.iium.edu.my/33507/ http://irep.iium.edu.my/33507/ http://irep.iium.edu.my/33507/ http://irep.iium.edu.my/33507/1/Palama_et_al.%2C_2009._JAFC.pdf

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spelling	iium-335072013-12-18T04:44:57Z http://irep.iium.edu.my/33507/ Metabolic changes in different developmental stages of vanilla planifolia pods Palama, Tony Lionel Khatib, Alfi Choi, Young Hae Payet, Bertrand Fock, Isabelle Verpoorte, Robert Kodja, Hippolyte S Agriculture (General) The metabolomic analysis of developing Vanilla planifolia green pods (between 3 and 8 months after pollination) was carried out by nuclear magnetic resonance (NMR) spectroscopy and multivariate data analysis. Multivariate data analysis of the 1H NMR spectra, such as principal component analysis (PCA) and partial least-squares-discriminant analysis (PLS-DA), showed a trend of separation of those samples based on the metabolites present in the methanol/water (1:1) extract. Older pods had a higher content of glucovanillin, vanillin, p-hydroxybenzaldehyde glucoside, p-hydroxybenzaldehyde, and sucrose, while younger pods had more bis[4-(β-D-glucopyranosyloxy)-benzyl]-2-isopropyltartrate (glucoside A), bis[4-(β-D-glucopyranosyloxy)-benzyl]-2-(2-butyl)tartrate (glucoside B), glucose, malic acid, and homocitric acid. A liquid chromatography-mass spectrometry (LC-MS) analysis targeted at phenolic compound content was also performed on the developing pods and confirmed the NMR results. Ratios of aglycones/glucosides were estimated and thus allowed for detection of more minor metabolites in the green vanilla pods. Quantification of compounds based on both LC-MS and NMR analyses showed that free vanillin can reach 24% of the total vanillin content after 8 months of development in the vanilla green pods. American Chemical Society 2009-05-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33507/1/Palama_et_al.%2C_2009._JAFC.pdf Palama, Tony Lionel and Khatib, Alfi and Choi, Young Hae and Payet, Bertrand and Fock, Isabelle and Verpoorte, Robert and Kodja, Hippolyte (2009) Metabolic changes in different developmental stages of vanilla planifolia pods. Journal of Agricultural and Food Chemistry, 57 (17). pp. 7651-7658. ISSN 0021-8561 http://pubs.acs.org/action/doSearch?action=search&searchText=Metabolic+changes+in+different+developmental+stages+of+vanilla+planifolia+pods&qsSearchArea=searchText&type=within&publication=40026026 10.1021/jf901508f
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	S Agriculture (General)
spellingShingle	S Agriculture (General) Palama, Tony Lionel Khatib, Alfi Choi, Young Hae Payet, Bertrand Fock, Isabelle Verpoorte, Robert Kodja, Hippolyte Metabolic changes in different developmental stages of vanilla planifolia pods
description	The metabolomic analysis of developing Vanilla planifolia green pods (between 3 and 8 months after pollination) was carried out by nuclear magnetic resonance (NMR) spectroscopy and multivariate data analysis. Multivariate data analysis of the 1H NMR spectra, such as principal component analysis (PCA) and partial least-squares-discriminant analysis (PLS-DA), showed a trend of separation of those samples based on the metabolites present in the methanol/water (1:1) extract. Older pods had a higher content of glucovanillin, vanillin, p-hydroxybenzaldehyde glucoside, p-hydroxybenzaldehyde, and sucrose, while younger pods had more bis[4-(β-D-glucopyranosyloxy)-benzyl]-2-isopropyltartrate (glucoside A), bis[4-(β-D-glucopyranosyloxy)-benzyl]-2-(2-butyl)tartrate (glucoside B), glucose, malic acid, and homocitric acid. A liquid chromatography-mass spectrometry (LC-MS) analysis targeted at phenolic compound content was also performed on the developing pods and confirmed the NMR results. Ratios of aglycones/glucosides were estimated and thus allowed for detection of more minor metabolites in the green vanilla pods. Quantification of compounds based on both LC-MS and NMR analyses showed that free vanillin can reach 24% of the total vanillin content after 8 months of development in the vanilla green pods.
format	Article
author	Palama, Tony Lionel Khatib, Alfi Choi, Young Hae Payet, Bertrand Fock, Isabelle Verpoorte, Robert Kodja, Hippolyte
author_facet	Palama, Tony Lionel Khatib, Alfi Choi, Young Hae Payet, Bertrand Fock, Isabelle Verpoorte, Robert Kodja, Hippolyte
author_sort	Palama, Tony Lionel
title	Metabolic changes in different developmental stages of vanilla planifolia pods
title_short	Metabolic changes in different developmental stages of vanilla planifolia pods
title_full	Metabolic changes in different developmental stages of vanilla planifolia pods
title_fullStr	Metabolic changes in different developmental stages of vanilla planifolia pods
title_full_unstemmed	Metabolic changes in different developmental stages of vanilla planifolia pods
title_sort	metabolic changes in different developmental stages of vanilla planifolia pods
publisher	American Chemical Society
publishDate	2009
url	http://irep.iium.edu.my/33507/ http://irep.iium.edu.my/33507/ http://irep.iium.edu.my/33507/ http://irep.iium.edu.my/33507/1/Palama_et_al.%2C_2009._JAFC.pdf
first_indexed	2023-09-18T20:48:26Z
last_indexed	2023-09-18T20:48:26Z
_version_	1777409832203059200

Metabolic changes in different developmental stages of vanilla planifolia pods

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