Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy

Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy

The metabolites of three species of Apiaceae, also known as Pegaga, were analyzed utilizing 1H NMR spectroscopy and multivariate data analysis. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) resolved the species, Centella asiatica, Hydrocotyle bonariensis, and Hydrocotyle...

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Main Authors: H., Maulidiani, Khatib, Alfi, Shaari, Khozirah, Abas, Faridah, Shitan, Mahendran, Kneer, Ralf, Neto, Victor, Lajis, Nurdin Hj
Format: Article
Language:English
Published: American Chemical Society 2012
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/33503/
http://irep.iium.edu.my/33503/
http://irep.iium.edu.my/33503/
http://irep.iium.edu.my/33503/1/Maulidiani_et_al.%2C_2012.pdf
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recordtype eprints
spelling iium-335032013-12-18T03:20:51Z http://irep.iium.edu.my/33503/ Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy H., Maulidiani Khatib, Alfi Shaari, Khozirah Abas, Faridah Shitan, Mahendran Kneer, Ralf Neto, Victor Lajis, Nurdin Hj QD Chemistry The metabolites of three species of Apiaceae, also known as Pegaga, were analyzed utilizing 1H NMR spectroscopy and multivariate data analysis. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) resolved the species, Centella asiatica, Hydrocotyle bonariensis, and Hydrocotyle sibthorpioides, into three clusters. The saponins, asiaticoside and madecassoside, along with chlorogenic acids were the metabolites that contributed most to the separation. Furthermore, the effects of growth-lighting condition to metabolite contents were also investigated. The extracts of C. asiatica grown in full-day light exposure exhibited a stronger radical scavenging activity and contained more triterpenes (asiaticoside and madecassoside), flavonoids, and chlorogenic acids as compared to plants grown in 50% shade. This study established the potential of using a combination of 1HNMRspectroscopy and multivariate data analyses in differentiating three closely related species and the effects of growth lighting, based on their metabolite contents and identification of the markers contributing to their differences. American Chemical Society 2012-03-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33503/1/Maulidiani_et_al.%2C_2012.pdf H., Maulidiani and Khatib, Alfi and Shaari, Khozirah and Abas, Faridah and Shitan, Mahendran and Kneer, Ralf and Neto, Victor and Lajis, Nurdin Hj (2012) Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy. Journal of Agricultural and Food Chemistry, 60 (1). pp. 410-417. ISSN 0021-8561 http://pubs.acs.org/doi/abs/10.1021/jf200270y#aff6 10.1021/jf200270y
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
H., Maulidiani
Khatib, Alfi
Shaari, Khozirah
Abas, Faridah
Shitan, Mahendran
Kneer, Ralf
Neto, Victor
Lajis, Nurdin Hj
Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy
description The metabolites of three species of Apiaceae, also known as Pegaga, were analyzed utilizing 1H NMR spectroscopy and multivariate data analysis. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) resolved the species, Centella asiatica, Hydrocotyle bonariensis, and Hydrocotyle sibthorpioides, into three clusters. The saponins, asiaticoside and madecassoside, along with chlorogenic acids were the metabolites that contributed most to the separation. Furthermore, the effects of growth-lighting condition to metabolite contents were also investigated. The extracts of C. asiatica grown in full-day light exposure exhibited a stronger radical scavenging activity and contained more triterpenes (asiaticoside and madecassoside), flavonoids, and chlorogenic acids as compared to plants grown in 50% shade. This study established the potential of using a combination of 1HNMRspectroscopy and multivariate data analyses in differentiating three closely related species and the effects of growth lighting, based on their metabolite contents and identification of the markers contributing to their differences.
format Article
author H., Maulidiani
Khatib, Alfi
Shaari, Khozirah
Abas, Faridah
Shitan, Mahendran
Kneer, Ralf
Neto, Victor
Lajis, Nurdin Hj
author_facet H., Maulidiani
Khatib, Alfi
Shaari, Khozirah
Abas, Faridah
Shitan, Mahendran
Kneer, Ralf
Neto, Victor
Lajis, Nurdin Hj
author_sort H., Maulidiani
title Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy
title_short Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy
title_full Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy
title_fullStr Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy
title_full_unstemmed Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy
title_sort discrimination of three pegaga (centella) varieties and determination of growth-lighting effects on metabolites content based on the chemometry of 1h nuclear magnetic resonance spectroscopy
publisher American Chemical Society
publishDate 2012
url http://irep.iium.edu.my/33503/
http://irep.iium.edu.my/33503/
http://irep.iium.edu.my/33503/
http://irep.iium.edu.my/33503/1/Maulidiani_et_al.%2C_2012.pdf
first_indexed 2023-09-18T20:48:26Z
last_indexed 2023-09-18T20:48:26Z
_version_ 1777409831926235136

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