Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus

The impact of tropical seasons (dry and wet) and growth stages (8, 10 and 12 weeks) of Cosmos caudatus on the antioxidant activity (AA), total phenolic content (TPC) as well as the level of bioactive compounds were evaluated using high performance liquid chromatography (HPLC). The plant morphology (...

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Main Authors: Mediani, Ahmed, Abas, Faridah, Tan, Chin Ping, Khatib, Alfi, Lajis, Nordin H.
Format: Article
Language:English
Published: Springer US 2012
Subjects:
Online Access:http://irep.iium.edu.my/33491/
http://irep.iium.edu.my/33491/
http://irep.iium.edu.my/33491/
http://irep.iium.edu.my/33491/1/Mediani_et_al.%2C_2012_PFHN.pdf
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spelling iium-334912013-12-18T00:41:56Z http://irep.iium.edu.my/33491/ Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus Mediani, Ahmed Abas, Faridah Tan, Chin Ping Khatib, Alfi Lajis, Nordin H. QD Chemistry The impact of tropical seasons (dry and wet) and growth stages (8, 10 and 12 weeks) of Cosmos caudatus on the antioxidant activity (AA), total phenolic content (TPC) as well as the level of bioactive compounds were evaluated using high performance liquid chromatography (HPLC). The plant morphology (plant height) also showed variation between the two seasons. Samples planted from June to August (during the dry season) exhibited a remarkably higher bioactivity and height than those planted from October to December (during the wet season). The samples that were harvested at eight weeks of age during the dry season showed the highest bioactivity with values of 26.04 g GAE/100 g and 22.1 μg/ml for TPC and IC50, respectively. Identification of phytochemical constituents in the C. caudatus extract was carried out by liquid chromatography coupled with diode array detection and electrospray tandem mass (LC-DAD-ESIMS/MS) technique and the confirmation of constituents was achieved by comparison with literature data and/or co-chromatography with authentic standards. Six compounds were indentified including quercetin 3-O-rhamnoside, quercetin 3-O-glucoside, rutin,quercetin 3-O-arabinofuranoside, quercetin 3-O-galactoside and chlorogenic acid. Their concentrations showed significant variance among the 8, 10 and 12-week-old herbs during both seasons. Springer US 2012-10-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33491/1/Mediani_et_al.%2C_2012_PFHN.pdf Mediani, Ahmed and Abas, Faridah and Tan, Chin Ping and Khatib, Alfi and Lajis, Nordin H. (2012) Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus. Plant Foods for Human Nutrition, 67 (4). pp. 344-350. ISSN 0921-9668 http://link.springer.com/article/10.1007%2Fs11130-012-0317-x 10.1007/s11130-012-0317-x
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Mediani, Ahmed
Abas, Faridah
Tan, Chin Ping
Khatib, Alfi
Lajis, Nordin H.
Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus
description The impact of tropical seasons (dry and wet) and growth stages (8, 10 and 12 weeks) of Cosmos caudatus on the antioxidant activity (AA), total phenolic content (TPC) as well as the level of bioactive compounds were evaluated using high performance liquid chromatography (HPLC). The plant morphology (plant height) also showed variation between the two seasons. Samples planted from June to August (during the dry season) exhibited a remarkably higher bioactivity and height than those planted from October to December (during the wet season). The samples that were harvested at eight weeks of age during the dry season showed the highest bioactivity with values of 26.04 g GAE/100 g and 22.1 μg/ml for TPC and IC50, respectively. Identification of phytochemical constituents in the C. caudatus extract was carried out by liquid chromatography coupled with diode array detection and electrospray tandem mass (LC-DAD-ESIMS/MS) technique and the confirmation of constituents was achieved by comparison with literature data and/or co-chromatography with authentic standards. Six compounds were indentified including quercetin 3-O-rhamnoside, quercetin 3-O-glucoside, rutin,quercetin 3-O-arabinofuranoside, quercetin 3-O-galactoside and chlorogenic acid. Their concentrations showed significant variance among the 8, 10 and 12-week-old herbs during both seasons.
format Article
author Mediani, Ahmed
Abas, Faridah
Tan, Chin Ping
Khatib, Alfi
Lajis, Nordin H.
author_facet Mediani, Ahmed
Abas, Faridah
Tan, Chin Ping
Khatib, Alfi
Lajis, Nordin H.
author_sort Mediani, Ahmed
title Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus
title_short Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus
title_full Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus
title_fullStr Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus
title_full_unstemmed Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus
title_sort influence of growth stage and season on the antioxidant constituents of cosmos caudatus
publisher Springer US
publishDate 2012
url http://irep.iium.edu.my/33491/
http://irep.iium.edu.my/33491/
http://irep.iium.edu.my/33491/
http://irep.iium.edu.my/33491/1/Mediani_et_al.%2C_2012_PFHN.pdf
first_indexed 2023-09-18T20:48:25Z
last_indexed 2023-09-18T20:48:25Z
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