Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus
The impact of tropical seasons (dry and wet) and growth stages (8, 10 and 12 weeks) of Cosmos caudatus on the antioxidant activity (AA), total phenolic content (TPC) as well as the level of bioactive compounds were evaluated using high performance liquid chromatography (HPLC). The plant morphology (...
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iium-334912013-12-18T00:41:56Z http://irep.iium.edu.my/33491/ Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus Mediani, Ahmed Abas, Faridah Tan, Chin Ping Khatib, Alfi Lajis, Nordin H. QD Chemistry The impact of tropical seasons (dry and wet) and growth stages (8, 10 and 12 weeks) of Cosmos caudatus on the antioxidant activity (AA), total phenolic content (TPC) as well as the level of bioactive compounds were evaluated using high performance liquid chromatography (HPLC). The plant morphology (plant height) also showed variation between the two seasons. Samples planted from June to August (during the dry season) exhibited a remarkably higher bioactivity and height than those planted from October to December (during the wet season). The samples that were harvested at eight weeks of age during the dry season showed the highest bioactivity with values of 26.04 g GAE/100 g and 22.1 μg/ml for TPC and IC50, respectively. Identification of phytochemical constituents in the C. caudatus extract was carried out by liquid chromatography coupled with diode array detection and electrospray tandem mass (LC-DAD-ESIMS/MS) technique and the confirmation of constituents was achieved by comparison with literature data and/or co-chromatography with authentic standards. Six compounds were indentified including quercetin 3-O-rhamnoside, quercetin 3-O-glucoside, rutin,quercetin 3-O-arabinofuranoside, quercetin 3-O-galactoside and chlorogenic acid. Their concentrations showed significant variance among the 8, 10 and 12-week-old herbs during both seasons. Springer US 2012-10-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33491/1/Mediani_et_al.%2C_2012_PFHN.pdf Mediani, Ahmed and Abas, Faridah and Tan, Chin Ping and Khatib, Alfi and Lajis, Nordin H. (2012) Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus. Plant Foods for Human Nutrition, 67 (4). pp. 344-350. ISSN 0921-9668 http://link.springer.com/article/10.1007%2Fs11130-012-0317-x 10.1007/s11130-012-0317-x |
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QD Chemistry Mediani, Ahmed Abas, Faridah Tan, Chin Ping Khatib, Alfi Lajis, Nordin H. Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus |
description |
The impact of tropical seasons (dry and wet) and growth stages (8, 10 and 12 weeks) of Cosmos caudatus on the antioxidant activity (AA), total phenolic content (TPC) as well as the level of bioactive compounds were evaluated using high performance liquid chromatography (HPLC). The plant morphology (plant height) also showed variation between the two seasons. Samples planted from June to August (during the dry season) exhibited a remarkably higher bioactivity and height than those planted from October to December (during the wet season). The samples that were harvested at eight weeks of age during the dry season showed the highest bioactivity with values of 26.04 g GAE/100 g and 22.1 μg/ml for TPC and IC50, respectively. Identification of phytochemical constituents in the C. caudatus extract was carried out by liquid chromatography coupled with diode array detection and electrospray tandem mass (LC-DAD-ESIMS/MS) technique and the confirmation of constituents was achieved by comparison with literature data and/or co-chromatography with authentic standards. Six compounds were indentified including quercetin 3-O-rhamnoside, quercetin 3-O-glucoside, rutin,quercetin 3-O-arabinofuranoside, quercetin 3-O-galactoside and chlorogenic acid. Their concentrations showed significant variance among the 8, 10 and 12-week-old herbs during both seasons. |
format |
Article |
author |
Mediani, Ahmed Abas, Faridah Tan, Chin Ping Khatib, Alfi Lajis, Nordin H. |
author_facet |
Mediani, Ahmed Abas, Faridah Tan, Chin Ping Khatib, Alfi Lajis, Nordin H. |
author_sort |
Mediani, Ahmed |
title |
Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus |
title_short |
Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus |
title_full |
Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus |
title_fullStr |
Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus |
title_full_unstemmed |
Influence of growth stage and season on the antioxidant constituents of Cosmos caudatus |
title_sort |
influence of growth stage and season on the antioxidant constituents of cosmos caudatus |
publisher |
Springer US |
publishDate |
2012 |
url |
http://irep.iium.edu.my/33491/ http://irep.iium.edu.my/33491/ http://irep.iium.edu.my/33491/ http://irep.iium.edu.my/33491/1/Mediani_et_al.%2C_2012_PFHN.pdf |
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2023-09-18T20:48:25Z |
last_indexed |
2023-09-18T20:48:25Z |
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