Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic activity
We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatog...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor and Francis
2010
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/33317/ http://irep.iium.edu.my/33317/ http://irep.iium.edu.my/33317/ http://irep.iium.edu.my/33317/1/khatib_et_al%2C_2010._JLCRT.pdf |
| Summary: | We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar=semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform=methanol (9=1) as the first mobile phase and ethyl acetate=chloroform=methanol (28=28=44) as the second
mobile phase. |
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