Cannabinoid CB1 receptor binding and acetylcholinesterase inhibitory activity of Sceletium tortuosum L.

The whole plant extract of plant Sceletium tortuosum, plant native to South Africa, has been known traditionally to have mood enhancing and stimulant properties. These properties have been confirmed before by proving serotonin-uptake inhibition activity. A further confirmation by using CB1 recepto...

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Main Authors:	Lubbe, A., Khatib, Alfi, Yuliana, Nancy Dewi, Jinap, Selamat, Verpoorte, Robert
Format:	Article
Language:	English
Published:	Universiti Putra Malaysia 2010
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/33307/ http://irep.iium.edu.my/33307/ http://irep.iium.edu.my/33307/1/Lubbe_et_al.%2C_2010_IFRJ.pdf

id	iium-33307
recordtype	eprints
spelling	iium-333072013-12-13T00:20:51Z http://irep.iium.edu.my/33307/ Cannabinoid CB1 receptor binding and acetylcholinesterase inhibitory activity of Sceletium tortuosum L. Lubbe, A. Khatib, Alfi Yuliana, Nancy Dewi Jinap, Selamat Verpoorte, Robert QD Chemistry The whole plant extract of plant Sceletium tortuosum, plant native to South Africa, has been known traditionally to have mood enhancing and stimulant properties. These properties have been confirmed before by proving serotonin-uptake inhibition activity. A further confirmation by using CB1 receptor binding assay has been performed in this study. The unfermented alkaloid extract was proved to posses a higher activity to bind CB1 receptor compared to that of the fermented one. GC-MS analysis confirmed that unfermented alkoloid extract contain more alkaloids than the fermented one. The methanol extract was also more active than the fermented one, suggesting that non-alkaloid compounds in this extract could posses this activity. An additional test to check wether this extract can improve cognitive function and memory was performed by acetylcholinesterase inhibitory assay. Both fermented and unfermented alkaloid extracts could inhibit acetylcholinesterase with IC50 being 0.303 mg/ml and 0.330 mg/ml, respectively. However, the major alkaloid in the extract, mesembrine, did not show inhibition of the enzyme. A TLC based test proved that other alkaloids in the extract were responsible to the activity Universiti Putra Malaysia 2010-05-19 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33307/1/Lubbe_et_al.%2C_2010_IFRJ.pdf Lubbe, A. and Khatib, Alfi and Yuliana, Nancy Dewi and Jinap, Selamat and Verpoorte, Robert (2010) Cannabinoid CB1 receptor binding and acetylcholinesterase inhibitory activity of Sceletium tortuosum L. International Food Research Journal, 17 (2). pp. 349-355. ISSN 1985-4668 http://www.ifrj.upm.edu.my/index.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Lubbe, A. Khatib, Alfi Yuliana, Nancy Dewi Jinap, Selamat Verpoorte, Robert Cannabinoid CB1 receptor binding and acetylcholinesterase inhibitory activity of Sceletium tortuosum L.
description	The whole plant extract of plant Sceletium tortuosum, plant native to South Africa, has been known traditionally to have mood enhancing and stimulant properties. These properties have been confirmed before by proving serotonin-uptake inhibition activity. A further confirmation by using CB1 receptor binding assay has been performed in this study. The unfermented alkaloid extract was proved to posses a higher activity to bind CB1 receptor compared to that of the fermented one. GC-MS analysis confirmed that unfermented alkoloid extract contain more alkaloids than the fermented one. The methanol extract was also more active than the fermented one, suggesting that non-alkaloid compounds in this extract could posses this activity. An additional test to check wether this extract can improve cognitive function and memory was performed by acetylcholinesterase inhibitory assay. Both fermented and unfermented alkaloid extracts could inhibit acetylcholinesterase with IC50 being 0.303 mg/ml and 0.330 mg/ml, respectively. However, the major alkaloid in the extract, mesembrine, did not show inhibition of the enzyme. A TLC based test proved that other alkaloids in the extract were responsible to the activity
format	Article
author	Lubbe, A. Khatib, Alfi Yuliana, Nancy Dewi Jinap, Selamat Verpoorte, Robert
author_facet	Lubbe, A. Khatib, Alfi Yuliana, Nancy Dewi Jinap, Selamat Verpoorte, Robert
author_sort	Lubbe, A.
title	Cannabinoid CB1 receptor binding and acetylcholinesterase inhibitory activity of Sceletium tortuosum L.
title_short	Cannabinoid CB1 receptor binding and acetylcholinesterase inhibitory activity of Sceletium tortuosum L.
title_full	Cannabinoid CB1 receptor binding and acetylcholinesterase inhibitory activity of Sceletium tortuosum L.
title_fullStr	Cannabinoid CB1 receptor binding and acetylcholinesterase inhibitory activity of Sceletium tortuosum L.
title_full_unstemmed	Cannabinoid CB1 receptor binding and acetylcholinesterase inhibitory activity of Sceletium tortuosum L.
title_sort	cannabinoid cb1 receptor binding and acetylcholinesterase inhibitory activity of sceletium tortuosum l.
publisher	Universiti Putra Malaysia
publishDate	2010
url	http://irep.iium.edu.my/33307/ http://irep.iium.edu.my/33307/ http://irep.iium.edu.my/33307/1/Lubbe_et_al.%2C_2010_IFRJ.pdf
first_indexed	2023-09-18T20:48:09Z
last_indexed	2023-09-18T20:48:09Z
_version_	1777409813749170176

Cannabinoid CB1 receptor binding and acetylcholinesterase inhibitory activity of Sceletium tortuosum L.

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