Comprehensive extraction method integrated with NMR metabolomics : a new bioactivity screening method for plants, adenosine A1 receptor binding compounds in orthosiphon stamineus Benth

A large number of plant metabolites has provided as an incomparable chemical source for drug development. However, the wide range of the polarity of metabolites has been a big obstacle for full use of the chemical diversity. The initial step conventional extraction method by a single solvent does no...

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Main Authors:	Yuliana, Nancy Dewi, Khatib, Alfi, Verpoorte, Robert, Choi, Young Hae
Format:	Article
Language:	English
Published:	ACS Publication 2011
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/33301/ http://irep.iium.edu.my/33301/ http://irep.iium.edu.my/33301/ http://irep.iium.edu.my/33301/1/Yuliana_et_al._2011.pdf

id	iium-33301
recordtype	eprints
spelling	iium-333012013-12-13T08:23:42Z http://irep.iium.edu.my/33301/ Comprehensive extraction method integrated with NMR metabolomics : a new bioactivity screening method for plants, adenosine A1 receptor binding compounds in orthosiphon stamineus Benth Yuliana, Nancy Dewi Khatib, Alfi Verpoorte, Robert Choi, Young Hae QD Chemistry A large number of plant metabolites has provided as an incomparable chemical source for drug development. However, the wide range of the polarity of metabolites has been a big obstacle for full use of the chemical diversity. The initial step conventional extraction method by a single solvent does not make use of all the metabolites contained in plants. Also, it takes a long time to confirm the target activity of a single compound because of tedious separation steps. To solve the problem, a new extraction method coupled to NMR-based metabolomics is applied to identify bioactive natural products. A comprehensive extraction method consisting of a continuous flow of solvent mixtures through plant material was developed to provide extracts with a wider chemical variety than those yielded with a single solvent extraction. As the model experiment, 1H NMR spectra of the extracts obtained from the comprehensive extraction of Orthosiphon stamineus were subjected to multivariate data analysis to find its adenosine A1 binding activity. On the basis of the results, two flavonoids from a large number of chemicals were clearly verified to show the adenosine A1 binding activity without any further purification steps. This method could provide a solution to the major drawbacks of natural products in drug development. ACS Publication 2011-07-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33301/1/Yuliana_et_al._2011.pdf Yuliana, Nancy Dewi and Khatib, Alfi and Verpoorte, Robert and Choi, Young Hae (2011) Comprehensive extraction method integrated with NMR metabolomics : a new bioactivity screening method for plants, adenosine A1 receptor binding compounds in orthosiphon stamineus Benth. Analytical Chemistry, 83 (17). pp. 6902-6906. ISSN 0003-2700 http://pubs.acs.org/doi/abs/10.1021/ac201458n?prevSearch=%255BTitle%253A%2BComprehensive%2BExtraction%2BMethod%2BIntegrated%2Bwith%2BNMR%2BMetabolomics%253A%2BA%2BNew%2BBioactivity%2BScreening%2BMethod%2Bfor%2BPlants%252C%2BAdenosine%2BA1%2BReceptor%2BBin 10.1021/ac201458n
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Yuliana, Nancy Dewi Khatib, Alfi Verpoorte, Robert Choi, Young Hae Comprehensive extraction method integrated with NMR metabolomics : a new bioactivity screening method for plants, adenosine A1 receptor binding compounds in orthosiphon stamineus Benth
description	A large number of plant metabolites has provided as an incomparable chemical source for drug development. However, the wide range of the polarity of metabolites has been a big obstacle for full use of the chemical diversity. The initial step conventional extraction method by a single solvent does not make use of all the metabolites contained in plants. Also, it takes a long time to confirm the target activity of a single compound because of tedious separation steps. To solve the problem, a new extraction method coupled to NMR-based metabolomics is applied to identify bioactive natural products. A comprehensive extraction method consisting of a continuous flow of solvent mixtures through plant material was developed to provide extracts with a wider chemical variety than those yielded with a single solvent extraction. As the model experiment, 1H NMR spectra of the extracts obtained from the comprehensive extraction of Orthosiphon stamineus were subjected to multivariate data analysis to find its adenosine A1 binding activity. On the basis of the results, two flavonoids from a large number of chemicals were clearly verified to show the adenosine A1 binding activity without any further purification steps. This method could provide a solution to the major drawbacks of natural products in drug development.
format	Article
author	Yuliana, Nancy Dewi Khatib, Alfi Verpoorte, Robert Choi, Young Hae
author_facet	Yuliana, Nancy Dewi Khatib, Alfi Verpoorte, Robert Choi, Young Hae
author_sort	Yuliana, Nancy Dewi
title	Comprehensive extraction method integrated with NMR metabolomics : a new bioactivity screening method for plants, adenosine A1 receptor binding compounds in orthosiphon stamineus Benth
title_short	Comprehensive extraction method integrated with NMR metabolomics : a new bioactivity screening method for plants, adenosine A1 receptor binding compounds in orthosiphon stamineus Benth
title_full	Comprehensive extraction method integrated with NMR metabolomics : a new bioactivity screening method for plants, adenosine A1 receptor binding compounds in orthosiphon stamineus Benth
title_fullStr	Comprehensive extraction method integrated with NMR metabolomics : a new bioactivity screening method for plants, adenosine A1 receptor binding compounds in orthosiphon stamineus Benth
title_full_unstemmed	Comprehensive extraction method integrated with NMR metabolomics : a new bioactivity screening method for plants, adenosine A1 receptor binding compounds in orthosiphon stamineus Benth
title_sort	comprehensive extraction method integrated with nmr metabolomics : a new bioactivity screening method for plants, adenosine a1 receptor binding compounds in orthosiphon stamineus benth
publisher	ACS Publication
publishDate	2011
url	http://irep.iium.edu.my/33301/ http://irep.iium.edu.my/33301/ http://irep.iium.edu.my/33301/ http://irep.iium.edu.my/33301/1/Yuliana_et_al._2011.pdf
first_indexed	2023-09-18T20:48:08Z
last_indexed	2023-09-18T20:48:08Z
_version_	1777409813012021248

Comprehensive extraction method integrated with NMR metabolomics : a new bioactivity screening method for plants, adenosine A1 receptor binding compounds in orthosiphon stamineus Benth

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