Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates

The amino-acid composition, 2, 2-Diphenyl-1-picryhydrazyl (DPPH) radical-scavenging activity, and peptide patterns of tilapia protein hydrolysates produced by the enzymatic hydrolysis of Alcalase (AH), Flavourzyme (FH) and Protamex (PH) for 5h using pH-stat method were studied. The ratio of essent...

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Main Authors: Shamloo, M., Bakar , Jamilah, Mat Hashim, D., Khatib, Alfi
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2012
Subjects:
Online Access:http://irep.iium.edu.my/33298/
http://irep.iium.edu.my/33298/
http://irep.iium.edu.my/33298/1/Shamloo_et_al._2012.pdf
id iium-33298
recordtype eprints
spelling iium-332982013-12-13T04:19:45Z http://irep.iium.edu.my/33298/ Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates Shamloo, M. Bakar , Jamilah Mat Hashim, D. Khatib, Alfi S Agriculture (General) The amino-acid composition, 2, 2-Diphenyl-1-picryhydrazyl (DPPH) radical-scavenging activity, and peptide patterns of tilapia protein hydrolysates produced by the enzymatic hydrolysis of Alcalase (AH), Flavourzyme (FH) and Protamex (PH) for 5h using pH-stat method were studied. The ratio of essential amino acids to non-essential amino acids increased after hydrolysis in all samples; however, no significant differences among them were observed. AH had a highest (P < 0.05) DPPH radical-scavenging activity, but no significant difference in the DPPH between FH and PH was observed. SDS-PAGE patterns for all the hydrolysates showed significant (P < 0.05) reduction in the number and the intensity of the bands with increasing time of hydrolysis. Flavourzyme showed the lowest rate of hydrolytic activity towards the tilapia mince. Faculty of Food Science and Technology, Universiti Putra Malaysia 2012-01-28 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33298/1/Shamloo_et_al._2012.pdf Shamloo, M. and Bakar , Jamilah and Mat Hashim, D. and Khatib, Alfi (2012) Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates. International Food Research Journal, 19 (1). pp. 183-188. ISSN 19854668 (P), 22317546 (O) http://www.ifrj.upm.edu.my/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Shamloo, M.
Bakar , Jamilah
Mat Hashim, D.
Khatib, Alfi
Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates
description The amino-acid composition, 2, 2-Diphenyl-1-picryhydrazyl (DPPH) radical-scavenging activity, and peptide patterns of tilapia protein hydrolysates produced by the enzymatic hydrolysis of Alcalase (AH), Flavourzyme (FH) and Protamex (PH) for 5h using pH-stat method were studied. The ratio of essential amino acids to non-essential amino acids increased after hydrolysis in all samples; however, no significant differences among them were observed. AH had a highest (P < 0.05) DPPH radical-scavenging activity, but no significant difference in the DPPH between FH and PH was observed. SDS-PAGE patterns for all the hydrolysates showed significant (P < 0.05) reduction in the number and the intensity of the bands with increasing time of hydrolysis. Flavourzyme showed the lowest rate of hydrolytic activity towards the tilapia mince.
format Article
author Shamloo, M.
Bakar , Jamilah
Mat Hashim, D.
Khatib, Alfi
author_facet Shamloo, M.
Bakar , Jamilah
Mat Hashim, D.
Khatib, Alfi
author_sort Shamloo, M.
title Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates
title_short Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates
title_full Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates
title_fullStr Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates
title_full_unstemmed Biochemical properties of red tilapia (Oreochromis niloticus) protein hydrolysates
title_sort biochemical properties of red tilapia (oreochromis niloticus) protein hydrolysates
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate 2012
url http://irep.iium.edu.my/33298/
http://irep.iium.edu.my/33298/
http://irep.iium.edu.my/33298/1/Shamloo_et_al._2012.pdf
first_indexed 2023-09-18T20:48:08Z
last_indexed 2023-09-18T20:48:08Z
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