Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)

The present study was conducted to assess the antioxidant properties of ethanol, ethyl acetate, methanol and hexane extracts from leaves of Pereskia bleo using 2,2-diphenyl-1-picrylhydrazyl method (DPPH assay), ferric reducing antioxidant power (FRAP assay) and β-Carotene-Linoleic acid methods. Tota...

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Main Authors: Hassanbaglou, Behnaz, Abdul Hamid, Azizah, A. M., Roheeyati, Mohd Saleh, Norihan, Abdulamir, AhmadSahib, Khatib, Alfi, Sabu, MC
Format: Article
Language:English
Published: Academic Journals 2012
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Online Access:http://irep.iium.edu.my/33294/
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http://irep.iium.edu.my/33294/1/Hassanbaglou_et_al_%282012%29.pdf
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spelling iium-332942013-12-13T08:34:15Z http://irep.iium.edu.my/33294/ Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae) Hassanbaglou, Behnaz Abdul Hamid, Azizah A. M., Roheeyati Mohd Saleh, Norihan Abdulamir, AhmadSahib Khatib, Alfi Sabu, MC QD Chemistry The present study was conducted to assess the antioxidant properties of ethanol, ethyl acetate, methanol and hexane extracts from leaves of Pereskia bleo using 2,2-diphenyl-1-picrylhydrazyl method (DPPH assay), ferric reducing antioxidant power (FRAP assay) and β-Carotene-Linoleic acid methods. Total phenolic compounds (TPC) and flavonoids of the leaves of P. bleo were measured using Folin-Ciocalteu and HPLC methods respectively. Results of the present study showed that Ethyl acetate extract of P. bleo leaf exhibited significantly (p < 0.05) higher antioxidant activity as measured using DPPH free radical (IC50), FRAP and β-carotene-linoleic assays than that of hexane extract, methanol extract and ethanol extract. Pereskia bleo leaf was also found to contain high amounts of bioactive compounds including total phenolic compounds (109.43 ±0.84 mg GAE/g), epicatechin (575± 0.04 mg/100g), quercetin (110.94 ±0.12 mg/100g), catechin (918 ± 0.01 mg/100 g) and myricetin (9.49±0.55 mg/100g) while the concentrations of α- tocopherol, β-carotene and lycopene were found to be higher (69±0.25, 51.97±1.18 mg/100 g, 92.46±0.41 μg/g), respectively. Academic Journals 2012-04-23 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33294/1/Hassanbaglou_et_al_%282012%29.pdf Hassanbaglou, Behnaz and Abdul Hamid, Azizah and A. M., Roheeyati and Mohd Saleh, Norihan and Abdulamir, AhmadSahib and Khatib, Alfi and Sabu, MC (2012) Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae). Journal of Medicinal Plants Research, 6 (15). pp. 2932-2937. ISSN 1996-0875 http://www.academicjournals.org/journal/JMPR/edition/23_April,_2012 10.5897/JMPR11.760
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Hassanbaglou, Behnaz
Abdul Hamid, Azizah
A. M., Roheeyati
Mohd Saleh, Norihan
Abdulamir, AhmadSahib
Khatib, Alfi
Sabu, MC
Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)
description The present study was conducted to assess the antioxidant properties of ethanol, ethyl acetate, methanol and hexane extracts from leaves of Pereskia bleo using 2,2-diphenyl-1-picrylhydrazyl method (DPPH assay), ferric reducing antioxidant power (FRAP assay) and β-Carotene-Linoleic acid methods. Total phenolic compounds (TPC) and flavonoids of the leaves of P. bleo were measured using Folin-Ciocalteu and HPLC methods respectively. Results of the present study showed that Ethyl acetate extract of P. bleo leaf exhibited significantly (p < 0.05) higher antioxidant activity as measured using DPPH free radical (IC50), FRAP and β-carotene-linoleic assays than that of hexane extract, methanol extract and ethanol extract. Pereskia bleo leaf was also found to contain high amounts of bioactive compounds including total phenolic compounds (109.43 ±0.84 mg GAE/g), epicatechin (575± 0.04 mg/100g), quercetin (110.94 ±0.12 mg/100g), catechin (918 ± 0.01 mg/100 g) and myricetin (9.49±0.55 mg/100g) while the concentrations of α- tocopherol, β-carotene and lycopene were found to be higher (69±0.25, 51.97±1.18 mg/100 g, 92.46±0.41 μg/g), respectively.
format Article
author Hassanbaglou, Behnaz
Abdul Hamid, Azizah
A. M., Roheeyati
Mohd Saleh, Norihan
Abdulamir, AhmadSahib
Khatib, Alfi
Sabu, MC
author_facet Hassanbaglou, Behnaz
Abdul Hamid, Azizah
A. M., Roheeyati
Mohd Saleh, Norihan
Abdulamir, AhmadSahib
Khatib, Alfi
Sabu, MC
author_sort Hassanbaglou, Behnaz
title Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)
title_short Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)
title_full Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)
title_fullStr Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)
title_full_unstemmed Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)
title_sort antioxidant activity of different extracts from leaves of pereskia bleo (cactaceae)
publisher Academic Journals
publishDate 2012
url http://irep.iium.edu.my/33294/
http://irep.iium.edu.my/33294/
http://irep.iium.edu.my/33294/
http://irep.iium.edu.my/33294/1/Hassanbaglou_et_al_%282012%29.pdf
first_indexed 2023-09-18T20:48:07Z
last_indexed 2023-09-18T20:48:07Z
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